Anti-beta III Tubulin antibody [2G10] - Neuronal Marker is a mouse monoclonal antibody that is used to detect beta III Tubulin in Flow cytometry (Intra), ICC/IF, IHC-P, IP, Western blot. Suitable for Catshark, Common marmoset, Dogfish, Human, Mouse, Rat samples.
- Specificity confirmed with Tubb3 knockout cell line validation
- Cited in over 320 publications
- Trusted since 2009
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
IHC-P | ICC/IF | IP | Flow Cyt (Intra) | WB | |
---|---|---|---|---|---|
Human | Tested | Tested | Expected | Tested | Tested |
Mouse | Tested | Tested | Tested | Expected | Tested |
Rat | Tested | Tested | Expected | Expected | Tested |
Cat | Predicted | Predicted | Predicted | Predicted | Predicted |
Catshark | Tested | Expected | Expected | Expected | Expected |
Chicken | Predicted | Predicted | Predicted | Predicted | Predicted |
Common marmoset | Tested | Expected | Expected | Expected | Expected |
Cow | Predicted | Predicted | Predicted | Predicted | Predicted |
Dogfish | Tested | Expected | Expected | Expected | Expected |
Quail | Predicted | Predicted | Predicted | Predicted | Predicted |
Rabbit | Predicted | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 0.20000-1.00000 µg/mL | Notes - |
Species Dogfish | Dilution info 0.20000-1.00000 µg/mL | Notes - |
Species Catshark | Dilution info 0.20000-1.00000 µg/mL | Notes - |
Species Common marmoset | Dilution info 0.20000-1.00000 µg/mL | Notes - |
Species Rat | Dilution info 0.20000-1.00000 µg/mL | Notes - |
Species Human | Dilution info 0.20000-1.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Chicken, Cow, Cat, Quail | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Dogfish, Catshark, Common marmoset | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Chicken, Cow, Cat, Quail | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Dogfish, Catshark, Common marmoset, Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Chicken, Cow, Cat, Quail | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Dogfish, Catshark, Common marmoset, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Chicken, Cow, Cat, Quail | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Dogfish, Catshark, Common marmoset | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Chicken, Cow, Cat, Quail | Dilution info - | Notes - |
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Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord.
Tubulin beta-3 chain, Neuron-specific class III beta-tubulin, Tubb3
Anti-beta III Tubulin antibody [2G10] - Neuronal Marker is a mouse monoclonal antibody that is used to detect beta III Tubulin in Flow cytometry (Intra), ICC/IF, IHC-P, IP, Western blot. Suitable for Catshark, Common marmoset, Dogfish, Human, Mouse, Rat samples.
- Specificity confirmed with Tubb3 knockout cell line validation
- Cited in over 320 publications
- Trusted since 2009
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) is a mouse monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB.
Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) was first used in a scientific publication in 1990 and has been cited over 323 times in peer reviewed journals. It's performance in Western blot and IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) has high sensitivity and specificity.
The specificity of Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) has been confirmed by WB testing in beta III Tubulin knockout HAP1 cells.
Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) has 40 independent reviews from customers.
Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) specifically detects beta III Tubulin (UniProt ID: Q4QRB4; Molecular weight: 51kDa) and is sold in 100 ug selling sizes.
Conjugation-ready, carrier free format available for antibody clone 2G10 - Anti-beta III Tubulin antibody [2G10] - BSA and Azide free ab264097.
Antibody clone 2G10 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, HRP (Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] - Neuronal Marker ab195879, ab19588, HRP Anti-beta III Tubulin antibody [2G10] - Neuronal Marker ab196638).
TUBB3, also known as beta-III tubulin, is a critical protein in neuro research, particularly for its role as a marker of neuronal differentiation and axonal growth. It is predominantly expressed in neurons and is essential for proper microtubule function and stability. Researchers use TUBB3 to study neuronal development, neurogenesis and the structural integrity of neurons, making it vital for understanding various neurodevelopmental and neurodegenerative conditions.
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Beta III tubulin often referred to as βIII tubulin or beta-tubulin 3 is a microtubule element involved in the cellular cytoskeleton structure. This protein with a molecular mass of approximately 55 kDa plays an important role in the development and maintenance of neuron structures. It is most prominently expressed in neurons within the central and peripheral nervous systems. In these cells beta III tubulin contributes to microtubule polymerization facilitating the formation of the intricate network necessary for cell shape intracellular transport and division.
Beta III tubulin acts in stabilizing microtubules which are essential for proper neuronal function. It exists as part of the tubulin dimer complex partnering with alpha-tubulin to form the building blocks of microtubules. This particular isotype is often used as a marker for neuronal differentiation due to its specific expression pattern in neural tissues. Its regulation and expression levels are important for neurogenesis and maintaining neuronal plasticity.
Beta III tubulin plays a role in neuron-specific intracellular transport and signaling pathways like the Rho GTPase and MAPK signaling pathways. These pathways are involved in cell cycle regulation and apoptotic processes. Relationships with other proteins such as kinesins and dyneins are important in these pathways influencing intracellular transport and signaling through binding and moving along the microtubule tracks created by beta-tubulin isotypes including beta III tubulin.
Abnormal beta III tubulin expression has been associated with cancer particularly in tumors originating from neuronal lineage such as glioblastomas. Overexpression or mutations can contribute to chemoresistance complicating treatment for certain types of cancer. Beta III tubulin is also linked to neurodevelopmental disorders as it affects proper neural network formation and stability. Its relationship with tumor protein p53 is noted in cancer pathways as p53 can influence beta III tubulin expression impacting cellular proliferation and apoptosis in oncogenic processes.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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ab78078 staining beta III Tubulin in primary rat neurons/glia, DIV14 (prepared from E18 rat hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. SDHEP) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab78078 at 1µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
(A-L) MHC (green) and β3-tubulin (red) co-immunostaining along the antero-posterior axis of the mouse soft palate at E13.5 (A-C), E14.5 (D-F), E16.5 (G-I), and P0 (J-L) at the level of the TVP and PLG (A, D, G, J), LVP (B, E, H, K), and PLP and SPC (C, F, I, L). Dashed lines indicate the outline of the palatal shelf and tongue epithelium. Arrows indicate the nerve fibers innervating the palatal shelves in a pattern complementary to the differentiated muscle, mainly located orally in the medial region. The schematic drawings indicate the orientation and the position of each section. Scale bars: 200μm.
beta III Tubulin is detected with ab78078.
(From Figure 4 of Grimaldi et al)
Immunohistochemistical detection of Neuron specific beta III Tubulin antibody [2G10] using ab78078 in formaldehyde-fixed paraffin-embedded quail embryo eye sections. Antigen retrieval step: heat mediated in citric acid pH6 buffer. Blocking step: 1% BSA for 10 mins @ rt°C Primary antibody incubated at 1/1250 for 2 hours in TBS/BSA/azide. Secondary antibody: anti-Mouse IgG conjugated to biotin used at 1/200. In this image, the lining cells of the hyaloid artery are positive. Excellent neural component specificity: even small fibres outside of the cartilage model of the orbit are clearly demonstrated.
Immunohistochemistical detection of Neuron specific beta III Tubulin using antibody (ab78078) on formaldehyde-fixed paraffin-embedded human medulla oblongata sections. Antigen retrieval step: Heat mediated in citric acid HIER buffer. Permeabilization: No. Blocking step: 1% BSA for 10 mins @ rt°C. Primary antibody dilution 1/250 incubated for 2 hours in TBS/BSA/azide. Secondary antibody: anti Mouse IgGs conjugated to biotin (1/200). This section was cut from an anonymous autopsy P.wax block that is over 20 yrs old, so I would expect variable positivity when compared with more recently sampled tissues (giving higher dilution factors, such as I obtained with fresh mouse/rat CNS blocks.) Transverse-cut axons stain very intensely longitudinal nerve processes and cell bodies are not so heavily stained but are still easily seen.
Neuron specific beta III Tubulin - Neuronal Marker was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Mouse monoclonal [2G10] to Neuron specific beta III Tubulin - Neuronal Marker and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab78078.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 50kDa: Neuron specific beta III Tubulin - Neuronal Marker.
All lanes: Immunoprecipitation - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078)
Predicted band size: 50 kDa
ab78078 was shown to specifically react with beta III Tubulin in wild-type HAP1 cells as signal was lost in beta III Tubulin knockout cells. Wild-type and beta III Tubulin knockout samples were subjected to SDS-PAGE. ab78078 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 10 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) at 1/20000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: Beta III Tubulin knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
Lane 4: HEK-293 whole cell lysate at 20 µg
Predicted band size: 50 kDa
ab78078 staining beta III Tubulin in NGF-differentiated PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab78078 at 1µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab78078 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406
All lanes: Western blot - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078) at 1 µg/mL
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: Brain (Mouse) Tissue Lysate at 10 µg
Lane 3: Brain (Rat) Tissue Lysate at 10 µg
All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 90s
ab78078 staining beta III Tubulin (red) in mouse differentiated neural stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100. Samples were incubated with primary antibody (5μg/ml in PBS + 3% BSA) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Blue - DAPI nuclear counterstain.
IHC image of ab78078 staining beta III Tubulin in Human cerebellum formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0 epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78078, 0.5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemical detection of Neuron specific beta III Tubulin using ab78078 on formaldehyde fixed dogfish/catshark PNS tissue sections. Antigen retrieval step: heat mediated in citric acid. Blocking: 1% BSA for 10 mins @ rt°C. Primary antibody ab78078 incubated at 1/1250 for 2 hours. Secondary Antibody: anti mouse IgG conjugated to biotin @ 1/200 This is a composite image of neuronal cell bodies and fibres; the upper image uses coloured arrowheads to indicate positive PNS components: cell bodies (black), L/S (green) and T/S (red) nerve fibres of what appear to be three Ganglia. Note that the L/S fibres do not appear as well-stained as the T/S fibres. The lower image shows what appears to be a linear sequence of single nerve cells and their processes within the core of a gill.
Immunohistochemistical detection of Neuron specific beta III Tubulin antibody using ab78078 on formaldehyde-fixed paraffin-embedded marmoset cerebellum sections. Antigen retrieval step: Heat mediated in Citric acid pH6 buffer. Permeabilization: No. Blocking step: 1% BSA for 10 mins @ rt°C. Primary Antibody used at 1/1000 incubated for 2 hours in TBS/BSA/azide. Secondary Antibody: anti mouse IgG conjugation to biotin (1/200).
ab78078 staining beta III Tubulin in Chicken e6: developing eye tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1250) for 2 hours. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Overlay histogram showing SH-SY5Y stained with ab78078 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78078, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.
IHC image of ab78078 staining beta III Tubulin in rat cerebellum formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with EDTA/Tris (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab78078, 0.5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
IHC image of ab78078 staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78078, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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