Mouse Monoclonal Beta-3-tubulin antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (AB264113), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-5g8-bsa-and-azide-free-ab264113--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123189.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (AB264113)")
![Western blot - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (AB264113), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-5g8-bsa-and-azide-free-ab264113--western-blot-img100428.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (AB264113)")
![Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (AB264113), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-5g8-bsa-and-azide-free-ab264113--immunocytochemistry-immunofluorescence-img121077.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (AB264113)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (AB264113), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-5g8-bsa-and-azide-free-ab264113--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101822.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (AB264113)")
Publications
Filter by
- Cancers 12:2020Reduced Tumorigenicity of Mouse ES Cells and the Augmented Anti-Tumor Therapeutic Effects under Deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuki Sonoda,Yuka Sasaki,Akemi Gunji,Hidenori Shirai,Tomonori Araki,Shoji Imamichi,Takae Onodera,Anna-Margareta Rydén,Masatoshi Watanabe,Jun Itami,Takuya Honda,Kazuto Ashizawa,Kazuhiko Nakao,Mitsuko MasutaniPubMed 32344695
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Tested | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
10 products for Alternative Product
1 product for Alternative Version
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).
Alternative names
TUBB4, TUBB3, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III
Recommended products
Mouse Monoclonal Beta-3-tubulin antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 5G8
- Purification technique
- Affinity purification
- Epitope
- The epitope recognized by 5G8 is EAQGPK
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab264113 is the carrier-free version of Anti-beta III Tubulin antibody [5G8] - Neuronal Marker ab231084.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta III tubulin often referred to as βIII tubulin or beta-tubulin 3 is a microtubule element involved in the cellular cytoskeleton structure. This protein with a molecular mass of approximately 55 kDa plays an important role in the development and maintenance of neuron structures. It is most prominently expressed in neurons within the central and peripheral nervous systems. In these cells beta III tubulin contributes to microtubule polymerization facilitating the formation of the intricate network necessary for cell shape intracellular transport and division.
- Biological function summary
Beta III tubulin acts in stabilizing microtubules which are essential for proper neuronal function. It exists as part of the tubulin dimer complex partnering with alpha-tubulin to form the building blocks of microtubules. This particular isotype is often used as a marker for neuronal differentiation due to its specific expression pattern in neural tissues. Its regulation and expression levels are important for neurogenesis and maintaining neuronal plasticity.
- Pathways
Beta III tubulin plays a role in neuron-specific intracellular transport and signaling pathways like the Rho GTPase and MAPK signaling pathways. These pathways are involved in cell cycle regulation and apoptotic processes. Relationships with other proteins such as kinesins and dyneins are important in these pathways influencing intracellular transport and signaling through binding and moving along the microtubule tracks created by beta-tubulin isotypes including beta III tubulin.
- Associated diseases and disorders
Abnormal beta III tubulin expression has been associated with cancer particularly in tumors originating from neuronal lineage such as glioblastomas. Overexpression or mutations can contribute to chemoresistance complicating treatment for certain types of cancer. Beta III tubulin is also linked to neurodevelopmental disorders as it affects proper neural network formation and stability. Its relationship with tumor protein p53 is noted in cancer pathways as p53 can influence beta III tubulin expression impacting cellular proliferation and apoptosis in oncogenic processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (ab264113), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-5g8-bsa-and-azide-free-ab264113--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123189.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (ab264113)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (ab264113)
Beta III Tubulin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-beta III Tubulin antibody
IHC image of beta III Tubulin staining in a section of formalin-fixed paraffin-embedded normal human cerebellum performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with Anti-beta III Tubulin antibody [5G8] - Neuronal Marker ab231084, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This data was developed using the same antibody clone in a different formulation containing PBS and Azide (Anti-beta III Tubulin antibody [5G8] - Neuronal Marker ab231084).
![Western blot - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (ab264113), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-5g8-bsa-and-azide-free-ab264113--western-blot-img100428.jpg "Western blot - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (ab264113)")
Western blot - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (ab264113)
Beta III Tubulin Western blot staining using mouse Anti-beta III Tubulin antibody
Anti-beta III Tubulin antibody [5G8] - Neuronal Marker ab231084 was shown to specifically react with beta III Tubulin (TUBB3) in wild type HAP1 cells. No band was observed when beta III Tubulin (TUBB3) knockout samples were used. Wild-type and beta III Tubulin (TUBB3) knockout samples were subjected to SDS-PAGE. Anti-beta III Tubulin antibody [5G8] - Neuronal Marker ab231084 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti GAPDH) were incubated overnight at 4°C at 1ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different formulation containing PBS and Azide (Anti-beta III Tubulin antibody [5G8] - Neuronal Marker ab231084).
All lanes: Western blot - Anti-beta III Tubulin antibody [5G8] - Neuronal Marker (Anti-beta III Tubulin antibody [5G8] - Neuronal Marker ab231084)
Lane 1: HAP1 whole cell lysate at 20 µg
Lane 2: HAP1 TUBB3 knockout whole cell lysate at 20 µg
Lane 3: Neuro2a whole cell lysate at 20 µg
Lane 4: PC12 whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 55 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (ab264113), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-5g8-bsa-and-azide-free-ab264113--immunocytochemistry-immunofluorescence-img121077.jpg "Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (ab264113)")
Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (ab264113)
Beta III Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-beta III Tubulin antibody
Anti-beta III Tubulin antibody [5G8] - Neuronal Marker ab231084 staining beta III tubulin (shown in green) in wild-type HAP1 cells (top panel) and TUBB3 knockout HAP1 cells (bottom panel).The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-beta III Tubulin antibody [5G8] - Neuronal Marker ab231084 at 1 μg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using the same antibody clone in a different formulation containing PBS and Azide (Anti-beta III Tubulin antibody [5G8] - Neuronal Marker ab231084).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (ab264113), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-5g8-bsa-and-azide-free-ab264113--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101822.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (ab264113)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [5G8] - BSA and Azide free (ab264113)
Beta III Tubulin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-beta III Tubulin antibody
IHC image of beta III Tubulin staining in a section of formalin-fixed paraffin-embedded normal rat cerebellum performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with Anti-beta III Tubulin antibody [5G8] - Neuronal Marker ab231084, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This data was developed using the same antibody clone in a different formulation containing PBS and Azide (Anti-beta III Tubulin antibody [5G8] - Neuronal Marker ab231084).
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