Skip to main content

Mouse Recombinant Monoclonal Beta-3-tubulin antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-beta III Tubulin antibody [AA10] (AB231083), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] (AB231083), expandable thumbnail
  • Western blot - Anti-beta III Tubulin antibody [AA10] (AB231083), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] (AB231083), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] (AB231083), expandable thumbnail

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).

    Alternative names

    Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB4, TUBB3

    Recommended products

    1. Loading...
    2. Loading...
    3. Loading...
    4. Loading...

    Mouse Recombinant Monoclonal Beta-3-tubulin antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples.

    Alternative names

    Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB4, TUBB3

    Key facts

    Isotype

    IgG2a

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Clone number

    AA10

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle

    Notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

    Supplementary info

    Activity summary

    Beta III tubulin often referred to as βIII tubulin or beta-tubulin 3 is a microtubule element involved in the cellular cytoskeleton structure. This protein with a molecular mass of approximately 55 kDa plays an important role in the development and maintenance of neuron structures. It is most prominently expressed in neurons within the central and peripheral nervous systems. In these cells beta III tubulin contributes to microtubule polymerization facilitating the formation of the intricate network necessary for cell shape intracellular transport and division.

    Biological function summary

    Beta III tubulin acts in stabilizing microtubules which are essential for proper neuronal function. It exists as part of the tubulin dimer complex partnering with alpha-tubulin to form the building blocks of microtubules. This particular isotype is often used as a marker for neuronal differentiation due to its specific expression pattern in neural tissues. Its regulation and expression levels are important for neurogenesis and maintaining neuronal plasticity.

    Pathways

    Beta III tubulin plays a role in neuron-specific intracellular transport and signaling pathways like the Rho GTPase and MAPK signaling pathways. These pathways are involved in cell cycle regulation and apoptotic processes. Relationships with other proteins such as kinesins and dyneins are important in these pathways influencing intracellular transport and signaling through binding and moving along the microtubule tracks created by beta-tubulin isotypes including beta III tubulin.

    Associated diseases and disorders

    Abnormal beta III tubulin expression has been associated with cancer particularly in tumors originating from neuronal lineage such as glioblastomas. Overexpression or mutations can contribute to chemoresistance complicating treatment for certain types of cancer. Beta III tubulin is also linked to neurodevelopmental disorders as it affects proper neural network formation and stability. Its relationship with tumor protein p53 is noted in cancer pathways as p53 can influence beta III tubulin expression impacting cellular proliferation and apoptosis in oncogenic processes.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    7 product images

    • Western blot - Anti-beta III Tubulin antibody [AA10] (ab231083), expandable thumbnail

      Western blot - Anti-beta III Tubulin antibody [AA10] (ab231083)

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Negative control: Human liver (PMID:20191564).

      All lanes: Western blot - Anti-beta III Tubulin antibody [AA10] (ab231083) at 1/1000 dilution

      Lane 1: Human brain tissue lysate at 10 µg

      Lane 2: Human heart tissue lysate at 10 µg

      Secondary

      All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

      Predicted band size: 50 kDa

      Exposure time: 3s

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Negative control: Human liver (PMID:20191564).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231083).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] (ab231083), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] (ab231083)

      Immunohistochemical analysis of paraffin-embedded human tonstil tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on human tonsil. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Western blot - Anti-beta III Tubulin antibody [AA10] (ab231083), expandable thumbnail

      Western blot - Anti-beta III Tubulin antibody [AA10] (ab231083)

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lane 1: 3 seconds; Lane 2-4: 1 second.

      All lanes: Western blot - Anti-beta III Tubulin antibody [AA10] (ab231083) at 1/1000 dilution

      Lane 1: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 10 µg

      Lane 2: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 10 µg

      Lane 3: Mouse brain tissue lysate at 10 µg

      Lane 4: Rat brain tissue lysate at 10 µg

      Secondary

      Lane 1: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

      Lanes 2 - 4: Western blot - Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) at 1/1000 dilution

      Predicted band size: 50 kDa

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lane 1: 3 seconds; Lane 2-4: 1 second.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231083).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] (ab231083), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] (ab231083)

      Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on rat cerebellum. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] (ab231083), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] (ab231083)

      Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on mouse cerebellum. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] (ab231083), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] (ab231083)

      Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on human cerebellum. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] (ab231083), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] (ab231083)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on human colon. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com