Anti-beta III Tubulin antibody [AA10] - Neuronal Marker

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - Neuronal Marker (AB231083)

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human cerebellum. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - Neuronal Marker (AB231083)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse cerebellum. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - Neuronal Marker (AB231083)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat cerebellum. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - Neuronal Marker (AB231083)

Immunohistochemical analysis of paraffin-embedded human tonstil tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human tonsil. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [AA10] - Neuronal Marker (AB231083)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling class III beta tubulin with ab231083 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human colon. The section was incubated with ab231083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• WB

Lab

Western blot - Anti-beta III Tubulin antibody [AA10] - Neuronal Marker (AB231083)

Blocking/Dilution buffer : 5% NFDM/TBST.

Negative control : Human liver (PMID : 20191564).

All lanes:

Western blot - Anti-beta III Tubulin antibody [AA10] - Neuronal Marker (ab231083) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 10 µg

Lane 2:

Human heart tissue lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 50 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Anti-beta III Tubulin antibody [AA10] - Neuronal Marker (AB231083)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 3 seconds; Lane 2-4 : 1 second.

All lanes:

Western blot - Anti-beta III Tubulin antibody [AA10] - Neuronal Marker (ab231083) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 10 µg

Lane 3:

Mouse brain tissue lysate at 10 µg

Lane 4:

Rat brain tissue lysate at 10 µg

Secondary

Lane 1:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lanes 2 - 4:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 50 kDa

false