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Rabbit Polyclonal Beta-3-tubulin antibody. C-terminal. Suitable for WB, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TUBB3.

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Images

Immunohistochemistry (Frozen sections) - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (AB230847), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - C-terminal (AB230847), expandable thumbnail
  • Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (AB230847), expandable thumbnail
  • Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (AB230847), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Synthetic Peptide within Human TUBB3. The exact immunogen used to generate this antibody is proprietary information. Database link Q13509

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-Fr
Human
Tested
Tested
Expected
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Tested
Chimpanzee
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted

Tested
Tested

Species
Mouse
Dilution info
1/5000 - 1/20000
Notes

-

Species
Rat
Dilution info
1/5000 - 1/20000
Notes

-

Species
Human
Dilution info
1/5000 - 1/20000
Notes

-

Predicted
Predicted

Species
Cow, Chimpanzee
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow, Chimpanzee
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/100 - 1/1000
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow, Chimpanzee
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).

Alternative names

Recommended products

Rabbit Polyclonal Beta-3-tubulin antibody. C-terminal. Suitable for WB, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TUBB3.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Synthetic Peptide within Human TUBB3. The exact immunogen used to generate this antibody is proprietary information. Database link Q13509
Purification technique
Affinity purification Immunogen
Concentration
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Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beta III tubulin often referred to as βIII tubulin or beta-tubulin 3 is a microtubule element involved in the cellular cytoskeleton structure. This protein with a molecular mass of approximately 55 kDa plays an important role in the development and maintenance of neuron structures. It is most prominently expressed in neurons within the central and peripheral nervous systems. In these cells beta III tubulin contributes to microtubule polymerization facilitating the formation of the intricate network necessary for cell shape intracellular transport and division.

Biological function summary

Beta III tubulin acts in stabilizing microtubules which are essential for proper neuronal function. It exists as part of the tubulin dimer complex partnering with alpha-tubulin to form the building blocks of microtubules. This particular isotype is often used as a marker for neuronal differentiation due to its specific expression pattern in neural tissues. Its regulation and expression levels are important for neurogenesis and maintaining neuronal plasticity.

Pathways

Beta III tubulin plays a role in neuron-specific intracellular transport and signaling pathways like the Rho GTPase and MAPK signaling pathways. These pathways are involved in cell cycle regulation and apoptotic processes. Relationships with other proteins such as kinesins and dyneins are important in these pathways influencing intracellular transport and signaling through binding and moving along the microtubule tracks created by beta-tubulin isotypes including beta III tubulin.

Associated diseases and disorders

Abnormal beta III tubulin expression has been associated with cancer particularly in tumors originating from neuronal lineage such as glioblastomas. Overexpression or mutations can contribute to chemoresistance complicating treatment for certain types of cancer. Beta III tubulin is also linked to neurodevelopmental disorders as it affects proper neural network formation and stability. Its relationship with tumor protein p53 is noted in cancer pathways as p53 can influence beta III tubulin expression impacting cellular proliferation and apoptosis in oncogenic processes.

Product promise

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4 product images

  • Immunohistochemistry (Frozen sections) - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (ab230847), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (ab230847)

    Beta III Tubulin Immunohistochemistry (Frozen sections) staining using rabbit Anti-beta III Tubulin antibody

    Frozen sectioned E13.5 rat brain tissue stained for beta III Tubulin (green) with ab230847 at 1/250 dilution in immunohistochemical analysis.

    Blue: Fluoroshield with DAPI

  • Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - C-terminal (ab230847), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - C-terminal (ab230847)

    SK-N-SH (human neuroblastoma cell line) cells stained for beta III Tubulin (green) using ab230847 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

  • Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (ab230847), expandable thumbnail

    Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (ab230847)

    Beta III Tubulin Western blot staining using rabbit Anti-beta III Tubulin antibody

    10% SDS-PAGE

    All lanes: Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (ab230847) at 1/10000 dilution

    Lane 1: Mouse brain tissue extract at 10 µg

    Lane 2: Rat brain tissue extract at 10 µg

    Developed using the ECL technique.

    Predicted band size: 50 kDa

  • Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (ab230847), expandable thumbnail

    Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (ab230847)

    Beta III Tubulin Western blot staining using rabbit Anti-beta III Tubulin antibody

    10% SDS-PAGE

    All lanes: Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (ab230847) at 1/10000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 20 µg

    Lane 2: SK-N-SH (human neuroblastoma cell line) whole cell extract at 20 µg

    Developed using the ECL technique.

    Predicted band size: 50 kDa

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Product protocols

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