AB230847

Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker

Name: Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (ab230847) | Abcam
Brand: Abcam Limited
SKU: AB230847
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Beta-3-tubulin antibody. C-terminal. Suitable for WB, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TUBB3.

View Alternative Names

TUBB4, TUBB3, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (AB230847)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (AB230847)

SK-N-SH (human neuroblastoma cell line) cells stained for beta III Tubulin (green) using ab230847 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Blue : Hoechst 33342 staining.

Immunohistochemistry (Frozen sections) - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (AB230847)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (AB230847)

Frozen sectioned E13.5 rat brain tissue stained for beta III Tubulin (green) with ab230847 at 1/250 dilution in immunohistochemical analysis.

Blue : Fluoroshield with DAPI

Supplier Data

Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (AB230847)

10% SDS-PAGE

All lanes:

Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (ab230847) at 1/10000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 20 µg

Lane 2:

SK-N-SH (human neuroblastoma cell line) whole cell extract at 20 µg

Predicted band size: 50 kDa

true

Supplier Data

Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (AB230847)

10% SDS-PAGE

All lanes:

Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (ab230847) at 1/10000 dilution

Lane 1:

Mouse brain tissue extract at 10 µg

Lane 2:

Rat brain tissue extract at 10 µg

Predicted band size: 50 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-Fr, WB

applications

Immunogen

Synthetic Peptide within Human TUBB3. The exact immunogen used to generate this antibody is proprietary information.

Q13509

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta III tubulin often referred to as βIII tubulin or beta-tubulin 3 is a microtubule element involved in the cellular cytoskeleton structure. This protein with a molecular mass of approximately 55 kDa plays an important role in the development and maintenance of neuron structures. It is most prominently expressed in neurons within the central and peripheral nervous systems. In these cells beta III tubulin contributes to microtubule polymerization facilitating the formation of the intricate network necessary for cell shape intracellular transport and division.

Biological function summary

Beta III tubulin acts in stabilizing microtubules which are essential for proper neuronal function. It exists as part of the tubulin dimer complex partnering with alpha-tubulin to form the building blocks of microtubules. This particular isotype is often used as a marker for neuronal differentiation due to its specific expression pattern in neural tissues. Its regulation and expression levels are important for neurogenesis and maintaining neuronal plasticity.

Pathways

Beta III tubulin plays a role in neuron-specific intracellular transport and signaling pathways like the Rho GTPase and MAPK signaling pathways. These pathways are involved in cell cycle regulation and apoptotic processes. Relationships with other proteins such as kinesins and dyneins are important in these pathways influencing intracellular transport and signaling through binding and moving along the microtubule tracks created by beta-tubulin isotypes including beta III tubulin.

Abnormal beta III tubulin expression has been associated with cancer particularly in tumors originating from neuronal lineage such as glioblastomas. Overexpression or mutations can contribute to chemoresistance complicating treatment for certain types of cancer. Beta III tubulin is also linked to neurodevelopmental disorders as it affects proper neural network formation and stability. Its relationship with tumor protein p53 is noted in cancer pathways as p53 can influence beta III tubulin expression impacting cellular proliferation and apoptosis in oncogenic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers (PubMed : 34996871, PubMed : 38305685, PubMed : 38609661). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed : 34996871, PubMed : 38305685, PubMed : 38609661). Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed : 34996871, PubMed : 38609661). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed : 20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed : 28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed : 28483977).

See full target information TUBB3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e24644 PubMed38390059

2024

Astragalus polysaccharide improves diabetic ulcers by promoting M2-polarization of macrophages to reduce excessive inflammation via the β-catenin/ NF-κB axis at the late phase of wound-healing.

Applications

Unspecified application

Species

Unspecified reactive species

Zhang Zhen,Shan Wei,Wang Yunfei,Xing Jie,Xu Jienan,Shen Yiting,Xiao Wen,Guo Shuyu,Liang Yue,Wang Xuanyu,Zhong Yumei,Que Huafa

Frontiers in cell and developmental biology 9:742714 PubMed34746137

2021

Syndromic Deafness Gene Controls Degeneration of Spiral Ganglion Neurons Through Modulating Proton Flux.

Applications

Unspecified application

Species

Unspecified reactive species

Shiwei Qiu,Weihao Zhao,Xue Gao,Dapeng Li,Weiqian Wang,Bo Gao,Weiju Han,Shiming Yang,Pu Dai,Peng Cao,Yongyi Yuan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (AB230847)

Immunohistochemistry (Frozen sections) - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (AB230847)

Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (AB230847)

Western blot - Anti-beta III Tubulin antibody - C-terminal - Neuronal Marker (AB230847)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise