Rabbit Recombinant Monoclonal Beta-3-tubulin antibody. Carrier free. Suitable for mIHC, IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 7 publications.
Images
![Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (AB221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--western-blot-img142493.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (AB221935)")
![Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (AB221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--immunocytochemistry-immunofluorescence-img120077.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (AB221935)")
![Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (AB221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--western-blot-img5275.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (AB221935)")
![Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (AB221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--western-blot-img141322.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (AB221935)")
![Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (AB221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--immunocytochemistry-immunofluorescence-img13136.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (AB221935)")
Publications
Filter by
- Biomaterials research 27:1012023Axon guidance gene-targeted siRNA delivery system improves neural stem cell transplantation therapy after spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeong Jun Kim,Wan-Kyu Ko,Gong Ho Han,Daye Lee,Min Jai Cho,Seung Hun Sheen,Seil SohnPubMed 37840145
- BMC cancer 15:5362015Overexpression of Class III β-tubulin, Sox2, and nuclear Survivin is predictive of taxane resistance in patients with stage III ovarian epithelial cancer.
- The Journal of clinical investigation 124:2560-702014Peripheral nervous system plasmalogens regulate Schwann cell differentiation and myelination.Applications:Unspecified applicationReactive species:MouseTiago Ferreira da Silva et. al.PubMed 24762439
- Anticancer research 33:5597-6022013Chemotherapy followed by surgery on the basis of biomarker examination for patients with advanced non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroyasu Yokomise,Dage Liu,Shinya Ishikawa,Tetsuhiko Go,Masashi Gotoh,Masaya Okuda,Shintaroh Tarumi,Yoshitaka Kasai,Natsumi MatsuuraPubMed 24324104
- The Journal of biological chemistry 288:35253-652013The growth factor receptor ERBB2 regulates mitochondrial activity on a signaling time scale.
- Anticancer research 33:1107-152013Biomarkers as prognostic factors for cN2 or 3 non-small cell lung cancer treated by induction chemoradiotherapy and surgery.Applications:Unspecified applicationReactive species:HumanHiroyasu Yokomise,Dage Liu,Sungsoo Chang,Tetsuhiko Go,Sinya Ishikawa,Nariyuki Misaki,Nariyasu NakashimaPubMed 23482788
- Cell 140:327-372010Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channel.Applications:ICC/IFReactive species:HumanPolina V Lishko,Inna L Botchkina,Andriy Fedorenko,Yuriy KirichokPubMed 20144758
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| mIHC | IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected | Expected |
| Rat | Expected | Expected | Tested | Tested | Expected |
| Zebrafish | Predicted | Predicted | Predicted | Predicted | Predicted |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
9 products for Alternative Version
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).
Alternative names
Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB4, TUBB3
Recommended products
Rabbit Recombinant Monoclonal Beta-3-tubulin antibody. Carrier free. Suitable for mIHC, IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 7 publications.
Alternative names
Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB4, TUBB3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP1569Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab221935 is the carrier-free version of Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta III tubulin often referred to as βIII tubulin or beta-tubulin 3 is a microtubule element involved in the cellular cytoskeleton structure. This protein with a molecular mass of approximately 55 kDa plays an important role in the development and maintenance of neuron structures. It is most prominently expressed in neurons within the central and peripheral nervous systems. In these cells beta III tubulin contributes to microtubule polymerization facilitating the formation of the intricate network necessary for cell shape intracellular transport and division.
- Biological function summary
Beta III tubulin acts in stabilizing microtubules which are essential for proper neuronal function. It exists as part of the tubulin dimer complex partnering with alpha-tubulin to form the building blocks of microtubules. This particular isotype is often used as a marker for neuronal differentiation due to its specific expression pattern in neural tissues. Its regulation and expression levels are important for neurogenesis and maintaining neuronal plasticity.
- Pathways
Beta III tubulin plays a role in neuron-specific intracellular transport and signaling pathways like the Rho GTPase and MAPK signaling pathways. These pathways are involved in cell cycle regulation and apoptotic processes. Relationships with other proteins such as kinesins and dyneins are important in these pathways influencing intracellular transport and signaling through binding and moving along the microtubule tracks created by beta-tubulin isotypes including beta III tubulin.
- Associated diseases and disorders
Abnormal beta III tubulin expression has been associated with cancer particularly in tumors originating from neuronal lineage such as glioblastomas. Overexpression or mutations can contribute to chemoresistance complicating treatment for certain types of cancer. Beta III tubulin is also linked to neurodevelopmental disorders as it affects proper neural network formation and stability. Its relationship with tumor protein p53 is noted in cancer pathways as p53 can influence beta III tubulin expression impacting cellular proliferation and apoptosis in oncogenic processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--western-blot-img142493.jpg "Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)")
Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)
This data was developed using the same antibody clone in a different buffer formulation (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623).
Lanes 1- 4: Merged signal (red and green). Green - Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 observed at 52 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 was shown to react with beta III Tubulin in wild-type HCT116 cells in western blot. Loss of signal was observed when knockout cell line Human TUBB3 (beta III Tubulin) knockout HCT116 cell line ab266900 (knockout cell lysate Human TUBB3 (beta III Tubulin) knockout HCT116 cell lysate ab257070) was used. Wild-type HCT116 and TUBB3 knockout HCT116 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623) at 1/1000 dilution
Lane 1: Wild-type HCT116 cell lysate at 20 µg
Lane 2: TUBB3 knockout HCT116 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 52 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--immunocytochemistry-immunofluorescence-img120077.jpg "Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)")
Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)
This ICC/IF data was generated using the same anti-beta III tubulin antibody clone, EP1569Y, in a different buffer formulation (cat# Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623).
Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 staining beta III Tubulin in wild-type HAP1 cells (top panel) and TUBB3 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 at 1/500 dilution and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--western-blot-img5275.jpg "Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)")
Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)
This data was developed using the same antibody clone in a different buffer formulation (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623).
Lanes 1- 2: Merged signal (red and green). Green - Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 observed at 50 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 was shown to react with beta III tubulin in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human TUBB3 (beta III Tubulin) knockout HeLa cell line ab255358 (knockout cell lysate Human TUBB3 (beta III Tubulin) knockout HeLa cell lysate ab263857) was used. Wild-type HeLa and TUBB3 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: TUBB3 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
![Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--western-blot-img141322.jpg "Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)")
Western blot - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)
This WB data was generated using the same anti-beta III tubulin antibody clone, EP1569Y, in a different buffer formulation (cat# Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 observed at 52 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 was shown to specifically react with beta III Tubulin in wild-type cells as signal was lost in beta III Tubulin knockout HAP1 cells. Wild-type and beta III Tubulin knockout samples were subjected to SDS-PAGE. Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: Beta III Tubulin knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
Lane 4: HEK293 whole cell lysate at 20 µg
Predicted band size: 50 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--immunocytochemistry-immunofluorescence-img13136.jpg "Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)")
Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)
Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 staining beta III Tubulin in NGF-differentiated PC12 cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 at 5μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135627.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)
Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623, at a 1/50 dilution, staining human class III beta Tubulin in breast carcinoma tissue using Immunohistochemistry, Paraffin embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--flow-cytometry-intracellular-img63760.jpg "Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)")
Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling beta III Tubulin with Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623).
![Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--flow-cytometry-intracellular-img51042.jpg "Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)")
Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)
Overlay histogram showing U-87MG cells stained with Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, 0.1μg/1x106) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in U-87MG cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img439532.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623).
Immunohistochemical analysis of formalin fixed paraffin embedded human breast carcinoma labelling beta III Tubulin with Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 at a concentration of 0.5 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH 8.5 for 32mins.
Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 anti-beta III Tubulin [EP1569Y] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135627.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)")
![Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--flow-cytometry-intracellular-img63760.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)")
![Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--flow-cytometry-intracellular-img51042.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935), expandable thumbnail](https://content.abcam.com/products/images/beta-iii-tubulin-antibody-ep1569y-bsa-and-azide-free-ab221935--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img439532.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EP1569Y] - BSA and Azide free (ab221935)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com