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Rabbit Recombinant Monoclonal Beta-3-tubulin antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (AB247375), expandable thumbnail
  • Western blot - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (AB247375), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (AB247375), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (AB247375), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (AB247375), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Tested
Expected
Rat
Expected
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

2 products for Alternative Version

Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).

Alternative names

TUBB4, TUBB3, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III

Recommended products

Rabbit Recombinant Monoclonal Beta-3-tubulin antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR1568Y
Purification technique
Affinity purification Protein A
Specificity

According to BLAST analysis, it is possible that the antibody will cross-react with TBB6 (Q9BUF5), TBB2B (Q9BVA1), TBB2A (Q13885), TBB5 (P07437), TBB4A (P04350), TBB4B (P68371). This cross-reactivity has not been confirmed experimentally.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247375 is the carrier-free version of Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beta III tubulin often referred to as βIII tubulin or beta-tubulin 3 is a microtubule element involved in the cellular cytoskeleton structure. This protein with a molecular mass of approximately 55 kDa plays an important role in the development and maintenance of neuron structures. It is most prominently expressed in neurons within the central and peripheral nervous systems. In these cells beta III tubulin contributes to microtubule polymerization facilitating the formation of the intricate network necessary for cell shape intracellular transport and division.

Biological function summary

Beta III tubulin acts in stabilizing microtubules which are essential for proper neuronal function. It exists as part of the tubulin dimer complex partnering with alpha-tubulin to form the building blocks of microtubules. This particular isotype is often used as a marker for neuronal differentiation due to its specific expression pattern in neural tissues. Its regulation and expression levels are important for neurogenesis and maintaining neuronal plasticity.

Pathways

Beta III tubulin plays a role in neuron-specific intracellular transport and signaling pathways like the Rho GTPase and MAPK signaling pathways. These pathways are involved in cell cycle regulation and apoptotic processes. Relationships with other proteins such as kinesins and dyneins are important in these pathways influencing intracellular transport and signaling through binding and moving along the microtubule tracks created by beta-tubulin isotypes including beta III tubulin.

Associated diseases and disorders

Abnormal beta III tubulin expression has been associated with cancer particularly in tumors originating from neuronal lineage such as glioblastomas. Overexpression or mutations can contribute to chemoresistance complicating treatment for certain types of cancer. Beta III tubulin is also linked to neurodevelopmental disorders as it affects proper neural network formation and stability. Its relationship with tumor protein p53 is noted in cancer pathways as p53 can influence beta III tubulin expression impacting cellular proliferation and apoptosis in oncogenic processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Western blot - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375), expandable thumbnail

    Western blot - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

    Beta III Tubulin Western blot staining of HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate using rabbit Anti-beta III Tubulin antibody

    This data was developed using Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker (Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193) at 1/50000 dilution

    All lanes: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa

    Observed band size: 50 kDa

  • Western blot - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375), expandable thumbnail

    Western blot - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

    Beta III Tubulin Western blot staining using rabbit Anti-beta III Tubulin antibody

    This data was developed using Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker (Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193) at 1/50000 dilution

    Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Human cerebellum lysate at 20 µg

    Lane 3: Mouse brain lysate at 20 µg

    Lane 4: Rat brain lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 50 kDa

    Observed band size: 50 kDa

  • Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

    Beta III Tubulin Flow Cytometry (Intracellular) staining using rabbit Anti-beta III Tubulin antibody

    This data was developed using Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling beta III Tubulin with Purified Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

    Beta III Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-beta III Tubulin antibody

    This data was developed using Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of Neuro-2a(Mouse neuroblastoma neuroblast) cells labeling beta III Tubulin with Purified Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193 at 1:50 dilution (2.2 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

    Beta III Tubulin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-beta III Tubulin antibody

    This data was developed using Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling beta III Tubulin with Purified Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193 at 1:8000 dilution (0.014 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

    Beta III Tubulin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-beta III Tubulin antibody

    This data was developed using Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling beta III Tubulin with Purified Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193 at 1:8000 dilution (0.014 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

    Beta III Tubulin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-beta III Tubulin antibody

    This data was developed using Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling beta III Tubulin with Purified Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193 at 1:8000 dilution (0.014 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

    Beta III Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-beta III Tubulin antibody

    This data was developed using Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193, the same antibody clone in a different buffer formulation.

    Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193 staining beta III Tubulin in wild-type HAP1 cells (top panel) and TUBB3 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193 at 1μg/ml concentration and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody, at 1μg/ml overnight at +4°C. This is followed by a further incubation at room temperature for 1h with a goat anti-rabbit IgG Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2μg/ml (shown in green) and a goat anti-mouse IgG Alexa Fluor® 647 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed ab150119) at 2μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

    This data was developed using Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193, the same antibody clone in a different buffer formulation.Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193 staining beta III Tubulin in Neuro-2a cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193 at 1μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
    Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

    This data was developed using Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193, the same antibody clone in a different buffer formulation.

    Overlay histogram showing HeLa cells stained with Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Anti-rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This antibody gave a positive signal in HeLa fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.

  • Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-beta III Tubulin antibody [EPR1568Y] - BSA and Azide free (ab247375)

    Beta III Tubulin Flow Cytometry (Intracellular) staining using rabbit Anti-beta III Tubulin antibody

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193).
    Flow cytometry overlay histogram showing wild-type HeLa (green line) and TUBB3 knockout HeLa stained with Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (Anti-beta III Tubulin antibody [EPR1568Y] - Neuronal Marker ab68193) (1x 106 in 100μl at 0.04 μg/ml (1/49250)) for 30min at 22°C.

    The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

    Isotype control antibody Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control was used at the same concentration and conditions as the primary antibody (wild-type HeLa - black line, TUBB3 knockout HeLa - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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