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Rabbit Polyclonal Beta-3-tubulin antibody. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P, WB and reacts with Human, Mouse, Rat, Catshark, Dogfish, Common marmoset samples. Cited in 628 publications.
Alternative names=Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB4, TUBB3
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| ICC/IF | Flow Cyt (Intra) | IHC-P | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Mouse | Tested | Tested | Tested | Tested |
| Rat | Tested | Expected | Tested | Tested |
| Catshark | Expected | Expected | Expected | Expected |
| Common marmoset | Expected | Expected | Expected | Expected |
| Dogfish | Expected | Expected | Expected | Expected |
| Pig | Predicted | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted | Predicted |
Images
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Catshark | Dilution info 1 µg/mL | Notes - |
Species Dogfish | Dilution info 1 µg/mL | Notes - |
Species Common marmoset | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Rhesus monkey | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.01 µg for 106 Cells | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody. |
Species Mouse | Dilution info 0.01 µg for 106 Cells | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Catshark | Dilution info 0.01 µg for 106 Cells | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody. |
Species Dogfish | Dilution info 0.01 µg for 106 Cells | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody. |
Species Common marmoset | Dilution info 0.01 µg for 106 Cells | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Species Common marmoset | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Catshark | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Dogfish | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Catshark | Dilution info 1 µg/mL | Notes - |
Species Dogfish | Dilution info 1 µg/mL | Notes - |
Species Common marmoset | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Rhesus monkey | Dilution info - | Notes - |
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).
Recommended products
Rabbit Polyclonal Beta-3-tubulin antibody. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P, WB and reacts with Human, Mouse, Rat, Catshark, Dogfish, Common marmoset samples. Cited in 628 publications.
Alternative names=Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB4, TUBB3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
The immunogen used for this product shares 75% homology with TUB (Tubby protein homolog, Uniprot: P50607). In western blot, we observe a specific band at ~55kDa which is not seen in KO cell lines. An additional band below this band of interest is seen at ~50kDa in both the WT and KO cells which could correspond to the protein TUB. Please note that cross-reactivity with this protein has not been confirmed experimentally. TUB is localized notably in high concentrations in the nucleoli of brain neurons with lower protein levels in the cytoplasm. Please, therefore, be aware that ICC experiments may need to be optimised. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. As an alternative antibody, we would recommend our recombinant rabbit monoclonal antibody ab52623 which has been shown to specific in both WB and ICC using KO cells.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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