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Rabbit Polyclonal Beta-3-tubulin antibody. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P, WB and reacts with Human, Mouse, Rat, Catshark, Dogfish, Common marmoset samples. Cited in 628 publications.

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)IHC-PWB
Human
Tested
Tested
Expected
Tested
Mouse
Tested
Tested
Tested
Tested
Rat
Tested
Expected
Tested
Tested
Catshark
Expected
Expected
Expected
Expected
Common marmoset
Expected
Expected
Expected
Expected
Dogfish
Expected
Expected
Expected
Expected
Pig
Predicted
Predicted
Predicted
Predicted
Rhesus monkey
Predicted
Predicted
Predicted
Predicted

Images

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Species
Rat
Dilution info
1 µg/mL
Notes

-

Expected
Expected

Species
Catshark
Dilution info
1 µg/mL
Notes

-

Species
Dogfish
Dilution info
1 µg/mL
Notes

-

Species
Common marmoset
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Pig, Rhesus monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
0.01 µg for 106 Cells
Notes

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody.

Species
Mouse
Dilution info
0.01 µg for 106 Cells
Notes

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody.

Expected
Expected

Species
Rat
Dilution info
-
Notes

-

Species
Catshark
Dilution info
0.01 µg for 106 Cells
Notes

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody.

Species
Dogfish
Dilution info
0.01 µg for 106 Cells
Notes

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody.

Species
Common marmoset
Dilution info
0.01 µg for 106 Cells
Notes

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody.

Predicted
Predicted

Species
Pig, Rhesus monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
-
Notes

-

Species
Common marmoset
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species
Catshark
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species
Dogfish
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Pig, Rhesus monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Species
Rat
Dilution info
1 µg/mL
Notes

-

Expected
Expected

Species
Catshark
Dilution info
1 µg/mL
Notes

-

Species
Dogfish
Dilution info
1 µg/mL
Notes

-

Species
Common marmoset
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Pig, Rhesus monkey
Dilution info
-
Notes

-

Target data

Recommended products

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Rabbit Polyclonal Beta-3-tubulin antibody. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P, WB and reacts with Human, Mouse, Rat, Catshark, Dogfish, Common marmoset samples. Cited in 628 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Purification technique

Affinity purification Immunogen

Specificity

The immunogen used for this product shares 75% homology with TUB (Tubby protein homolog, Uniprot: P50607). In western blot, we observe a specific band at ~55kDa which is not seen in KO cell lines. An additional band below this band of interest is seen at ~50kDa in both the WT and KO cells which could correspond to the protein TUB. Please note that cross-reactivity with this protein has not been confirmed experimentally. TUB is localized notably in high concentrations in the nucleoli of brain neurons with lower protein levels in the cytoplasm. Please, therefore, be aware that ICC experiments may need to be optimised. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. As an alternative antibody, we would recommend our recombinant rabbit monoclonal antibody ab52623 which has been shown to specific in both WB and ICC using KO cells.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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