AB41489

Anti-beta III Tubulin antibody - Neuronal Marker

Name: Anti-beta III Tubulin antibody - Neuronal Marker (ab41489) | Abcam
Brand: Abcam Limited
SKU: AB41489
Price: 480 USD
Availability: InStock
Rating: 2 (7 reviews)

(7 Reviews)

(98 Publications)

Anti-beta III Tubulin antibody (ab41489) is a chicken polyclonal antibody detecting beta III Tubulin in ICC/IF. Suitable for Mouse.

- Over 60 publications
- Trusted since 2006

View Alternative Names

TUBB4, TUBB3, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB41489)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB41489)

Immunocytochemistry/Immunofluorescence analysis of SpH64 cells in culture labelling beta III Tubulin (green) with ab41489. Cells were fixed with 4% paraformaldehyde. Red - Rabbit anti-GFP, Blue : DAPI.

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB41489)

Immunofluorescence analysis of HPC and PBMC blood-derived iPSC clones, staining beta III Tubulin (green) with ab41489.

Cells were fixed with 4% PFA and blocked for 30 minutes in PBS with 5% fetal bovine serum. Cells were incubated with primary antibody overnight at 4°C. A FITC-conjugated donkey anti-chicken IgG was used as the secondary antibody.

Image from Ohmine S et al., Stem Cell Research & Therapy 2011, 2:46, 16 November 2011. Fig 5.; doi: 10.1186/scrt87.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB41489)

Immunocytochemistry/Immunofluorescence analysis of SpH21 cells in culture labelling beta III Tubulin (green) with ab41489. Cells were fixed with 4% paraformaldehyde. Red - Rabbit anti-GAD67, Blue - DAPI.

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human TUBB3 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q13509

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000 - 1/2000", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-beta III Tubulin antibody (ab41489) is a chicken polyclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

Trusted by the scientific community
Anti-beta III Tubulin (ab41489) was first used in a scientific publication in 2006 and has been cited over 60 times in peer-reviewed journals.

Reviewed by scientists
Anti-beta III Tubulin (ab41489) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: 1X PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta III tubulin often referred to as ΒIII tubulin or beta-tubulin 3 is a microtubule element involved in the cellular cytoskeleton structure. This protein with a molecular mass of approximately 55 kDa plays an important role in the development and maintenance of neuron structures. It is most prominently expressed in neurons within the central and peripheral nervous systems. In these cells beta III tubulin contributes to microtubule polymerization facilitating the formation of the intricate network necessary for cell shape intracellular transport and division.

Biological function summary

Beta III tubulin acts in stabilizing microtubules which are essential for proper neuronal function. It exists as part of the tubulin dimer complex partnering with alpha-tubulin to form the building blocks of microtubules. This particular isotype is often used as a marker for neuronal differentiation due to its specific expression pattern in neural tissues. Its regulation and expression levels are important for neurogenesis and maintaining neuronal plasticity.

Pathways

Beta III tubulin plays a role in neuron-specific intracellular transport and signaling pathways like the Rho GTPase and MAPK signaling pathways. These pathways are involved in cell cycle regulation and apoptotic processes. Relationships with other proteins such as kinesins and dyneins are important in these pathways influencing intracellular transport and signaling through binding and moving along the microtubule tracks created by beta-tubulin isotypes including beta III tubulin.

Abnormal beta III tubulin expression has been associated with cancer particularly in tumors originating from neuronal lineage such as glioblastomas. Overexpression or mutations can contribute to chemoresistance complicating treatment for certain types of cancer. Beta III tubulin is also linked to neurodevelopmental disorders as it affects proper neural network formation and stability. Its relationship with tumor protein p53 is noted in cancer pathways as p53 can influence beta III tubulin expression impacting cellular proliferation and apoptosis in oncogenic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers (PubMed : 34996871, PubMed : 38305685, PubMed : 38609661). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed : 34996871, PubMed : 38305685, PubMed : 38609661). Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed : 34996871, PubMed : 38609661). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed : 20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed : 28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed : 28483977).

See full target information TUBB3

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Publications (98)

Recent publications for all applications. Explore the full list and refine your search

Nature 646:462-473 PubMed40836096

2025

Cancer-induced nerve injury promotes resistance to anti-PD-1 therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Erez N Baruch,Frederico O Gleber-Netto,Priyadharsini Nagarajan,Xiayu Rao,Shamima Akhter,Tuany Eichwald,Tongxin Xie,Mohammad Balood,Adebayo Adewale,Shorook Naara,Hinduja N Sathishkumar,Shajedul Islam,William McCarthy,Brandi J Mattson,Renata Ferrarotto,Michael K Wong,Michael A Davies,Sonali Jindal,Sreyashi Basu,Karine Roversi,Amin Reza Nikpoor,Maryam Ahmadi,Ali Ahmadi,Catherine Harwood,Irene Leigh,Dennis Gong,Paulino Tallón de Lara,Derrick L Tao,Tara M Davidson,Nadim J Ajami,Andrew Futreal,Kunal Rai,Veena Kochat,Micah Castillo,Preethi Gunaratne,Ryan P Goepfert,Sharia D Hernandez,Nikhil I Khushalani,Jing Wang,Stephanie S Watowich,George A Calin,Michael R Migden,Mona Yuan,Naijiang Liu,Yi Ye,William L Hwang,Paola D Vermeer,Nisha J D'Silva,Yuri L Bunimovich,Dan Yaniv,Jared K Burks,Javier Gomez,Patrick M Dougherty,Kenneth Y Tsai,James P Allison,Padmanee Sharma,Jennifer A Wargo,Jeffrey N Myers,Sebastien Talbot,Neil D Gross,Moran Amit

Stem cell research & therapy 16:406 PubMed40713855

2025

Pulsed electromagnetic stimulation promotes neuronal maturation by up-regulating cholesterol biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Chen,Jingyi Li,Vsevolod Telezhkin,Yu Gu,Min Tao,Liping Guo,Simin Song,Rihe Dong,Xianyang Luo,Yan Wang,Qian Liu,Weiming Tian,Weihua Meng,Wei Hong,Bing Song

Molecules and cells 47:100159 PubMed39577744

2024

Dipeptidyl peptidase 4 as an injury-responsive protein in the mouse sciatic nerve.

Applications

Unspecified application

Species

Unspecified reactive species

Yeonsoo Oh,Yongcheol Cho

Nature communications 15:9547 PubMed39528508

2024

Revealing single-neuron and network-activity interaction by combining high-density microelectrode array and optogenetics.

Applications

Unspecified application

Species

Unspecified reactive species

Toki Kobayashi,Kenta Shimba,Taiyo Narumi,Takahiro Asahina,Kiyoshi Kotani,Yasuhiko Jimbo

Science advances 10:eadq3539 PubMed39485845

2024

Mouse α-synuclein fibrils are structurally and functionally distinct from human fibrils associated with Lewy body diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Arpine Sokratian,Ye Zhou,Meltem Tatli,Kevin J Burbidge,Enquan Xu,Elizabeth Viverette,Sonia Donzelli,Addison M Duda,Yuan Yuan,Huizhong Li,Samuel Strader,Nirali Patel,Lauren Shiell,Tuyana Malankhanova,Olivia Chen,Joseph R Mazzulli,Lalith Perera,Henning Stahlberg,Mario Borgnia,Alberto Bartesaghi,Hilal A Lashuel,Andrew B West

Molecular psychiatry 30:1479-1496 PubMed39349966

2024

Dysregulation of miRNA expression and excitation in MEF2C autism patient hiPSC-neurons and cerebral organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Dorit Trudler,Swagata Ghatak,Michael Bula,James Parker,Maria Talantova,Melissa Luevanos,Sergio Labra,Titas Grabauskas,Sarah Moore Noveral,Mayu Teranaka,Emily Schahrer,Nima Dolatabadi,Clare Bakker,Kevin Lopez,Abdullah Sultan,Parth Patel,Agnes Chan,Yongwook Choi,Riki Kawaguchi,Pawel Stankiewicz,Ivan Garcia-Bassets,Piotr Kozbial,Michael G Rosenfeld,Nobuki Nakanishi,Daniel H Geschwind,Shing Fai Chan,Wei Lin,Nicholas J Schork,Rajesh Ambasudhan,Stuart A Lipton

Nature communications 15:6367 PubMed39112449

2024

Male autism spectrum disorder is linked to brain aromatase disruption by prenatal BPA in multimodal investigations and 10HDA ameliorates the related mouse phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Christos Symeonides,Kristina Vacy,Sarah Thomson,Sam Tanner,Hui Kheng Chua,Shilpi Dixit,Toby Mansell,Martin O'Hely,Boris Novakovic,Julie B Herbstman,Shuang Wang,Jia Guo,Jessalynn Chia,Nhi Thao Tran,Sang Eun Hwang,Kara Britt,Feng Chen,Tae Hwan Kim,Christopher A Reid,Anthony El-Bitar,Gabriel B Bernasochi,Lea M Durham Delbridge,Vincent R Harley,Yann W Yap,Deborah Dewey,Chloe J Love,David Burgner,Mimi L K Tang,Peter D Sly,Richard Saffery,Jochen F Mueller,Nicole Rinehart,Bruce Tonge,Peter Vuillermin,Anne-Louise Ponsonby,Wah Chin Boon

NPJ Parkinson's disease 10:138 PubMed39069518

2024

Human tripartite cortical network model for temporal assessment of alpha-synuclein aggregation and propagation in Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fikret Emre Kapucu,Iisa Tujula,Oskari Kulta,Lassi Sukki,Tomi Ryynänen,Hjalte Gram,Valtteri Vuolanto,Andrey Vinogradov,Joose Kreutzer,Poul Henning Jensen,Pasi Kallio,Susanna Narkilahti

Genes to cells : devoted to molecular & cellular mechanisms 29:746-756 PubMed38964745

2024

An N-terminal and ankyrin repeat domain interactome of Shank3 identifies the protein complex with the splicing regulator Nono in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sayaka Okuzono,Fumihiko Fujii,Daiki Setoyama,Ryoji Taira,Yohei Shinmyo,Hiroki Kato,Keiji Masuda,Kousuke Yonemoto,Satoshi Akamine,Yuki Matsushita,Yoshitomo Motomura,Takeshi Sakurai,Hiroshi Kawasaki,Kihoon Han,Takahiro A Kato,Hiroyuki Torisu,Dongchon Kang,Yusaku Nakabeppu,Shouichi Ohga,Yasunari Sakai

Nature communications 15:4819 PubMed38844464

2024

m6a methylation orchestrates IMP1 regulation of microtubules during human neuronal differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre Klein,Marija Petrić Howe,Jasmine Harley,Harry Crook,Sofia Esteban Serna,Theodoros I Roumeliotis,Jyoti S Choudhary,Anob M Chakrabarti,Raphaëlle Luisier,Rickie Patani,Andres Ramos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta III Tubulin antibody - Neuronal Marker

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB41489)

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB41489)

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB41489)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (98)

Product promise