Anti-beta III Tubulin antibody ab41489 is a chicken polyclonal antibody that is used in beta III Tubulin immunofluorescence. Suitable for mouse samples.
- Tried and trusted by researchers since 2006
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- ASN neuro 15:175909142311975272023SOD1 Astrocyte-Derived Extracellular Vesicles Induce Motor Neuron Death by a miRNA-155-5p-Mediated Mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoledad Marton,Ernesto Miquel,Joaquín Acosta-Rodríguez,Santiago Fontenla,Gabriela Libisch,Patricia CassinaPubMed 37644868
- Reproductive toxicology (Elmsford, N.Y.) 117:1083582023Assessment of neurotransmitter release in human iPSC-derived neuronal/glial cells: a missing in vitro assay for regulatory developmental neurotoxicity testing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiara Cervetto,Francesca Pistollato,Sarah Amato,Emilio Mendoza-de Gyves,Anna Bal-Price,Guido Maura,Manuela MarcoliPubMed 36863571
- Neuronal signaling 7:NS202200982023imaging of axonal transport in peripheral nerves of rodent forelimbs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiuhan Lang,Giampietro Schiavo,James N SleighPubMed 36743438
- Proceedings of the National Academy of Sciences of the United States of America 119:e22034991192022Nano-positioning and tubulin conformation contribute to axonal transport regulation of mitochondria along microtubules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValérie Van Steenbergen,Flavie Lavoie-Cardinal,Youcef Kazwiny,Marianna Decet,Tobie Martens,Patrik Verstreken,Werend Boesmans,Paul De Koninck,Pieter Vanden BerghePubMed 36322761
- The Journal of neuroscience : the official journal of the Society for Neuroscience 42:7513-75292022HCN2 Ion Channels Drive Pain in Rodent Models of Migraine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristoforos Tsantoulas,Aidan Ng,Larissa Pinto,Anna P Andreou,Peter A McNaughtonPubMed 36658457
- Nature 609:128-1352022Endocytosis in the axon initial segment maintains neuronal polarity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelsie Eichel,Takeshi Uenaka,Vivek Belapurkar,Rui Lu,Shouqiang Cheng,Joseph S Pak,Caitlin A Taylor,Thomas C Südhof,Robert Malenka,Marius Wernig,Engin Özkan,David Perrais,Kang ShenPubMed 35978188
- Development (Cambridge, England) 149:2022Practical guide for preparation, computational reconstruction and analysis of 3D human neuronal networks in control and ischaemic conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoora Räsänen,Venla Harju,Tiina Joki,Susanna NarkilahtiPubMed 35929583
- Current research in neurobiology 3:1000422022Plasticity of cell proliferation in the retina of fish under light and darkness conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInés Berrosteguieta,Juan Carlos Rosillo,María Laura Herrera,Silvia Olivera-Bravo,Gabriela Casanova,Vicente Herranz-Pérez,José Manuel García-Verdugo,Anabel Sonia FernándezPubMed 36518338
- Frontiers in integrative neuroscience 16:8550712022Validation of Functional Connectivity of Engineered Neuromuscular Junction With Recombinant Monosynaptic Pseudotyped ΔG-Rabies Virus Tracing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUlrich Stefan Bauer,Vegard Fiskum,Rajeevkumar Raveendran Nair,Rosanne van de Wijdeven,Clifford Kentros,Ioanna Sandvig,Axel SandvigPubMed 35669734
- Reproductive toxicology (Elmsford, N.Y.) 111:34-482022Effects of spike protein and toxin-like peptides found in COVID-19 patients on human 3D neuronal/glial model undergoing differentiation: Possible implications for SARS-CoV-2 impact on brain development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancesca Pistollato,Mauro Petrillo,Laure-Alix Clerbaux,Gabriele Leoni,Jessica Ponti,Alessia Bogni,Carlo Brogna,Simone Cristoni,Remo Sanges,Emilio Mendoza-de Gyves,Marco Fabbri,Maddalena Querci,Helena Soares,Amalia Munoz,Maurice Whelan,Guy Van de EedePubMed 35525527
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 99.98% 1X PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TUBB3 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q13509
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Mouse | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
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Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).
Alternative names
TUBB4, TUBB3, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III
Recommended products
Anti-beta III Tubulin antibody ab41489 is a chicken polyclonal antibody that is used in beta III Tubulin immunofluorescence. Suitable for mouse samples.
- Tried and trusted by researchers since 2006
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 99.98% 1X PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TUBB3 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q13509
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Equal volumes of three different affinity-purified anti-peptide antibodies were combined to make this product.
Do not freeze antibody unless you want to store for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta III tubulin often referred to as βIII tubulin or beta-tubulin 3 is a microtubule element involved in the cellular cytoskeleton structure. This protein with a molecular mass of approximately 55 kDa plays an important role in the development and maintenance of neuron structures. It is most prominently expressed in neurons within the central and peripheral nervous systems. In these cells beta III tubulin contributes to microtubule polymerization facilitating the formation of the intricate network necessary for cell shape intracellular transport and division.
- Biological function summary
Beta III tubulin acts in stabilizing microtubules which are essential for proper neuronal function. It exists as part of the tubulin dimer complex partnering with alpha-tubulin to form the building blocks of microtubules. This particular isotype is often used as a marker for neuronal differentiation due to its specific expression pattern in neural tissues. Its regulation and expression levels are important for neurogenesis and maintaining neuronal plasticity.
- Pathways
Beta III tubulin plays a role in neuron-specific intracellular transport and signaling pathways like the Rho GTPase and MAPK signaling pathways. These pathways are involved in cell cycle regulation and apoptotic processes. Relationships with other proteins such as kinesins and dyneins are important in these pathways influencing intracellular transport and signaling through binding and moving along the microtubule tracks created by beta-tubulin isotypes including beta III tubulin.
- Associated diseases and disorders
Abnormal beta III tubulin expression has been associated with cancer particularly in tumors originating from neuronal lineage such as glioblastomas. Overexpression or mutations can contribute to chemoresistance complicating treatment for certain types of cancer. Beta III tubulin is also linked to neurodevelopmental disorders as it affects proper neural network formation and stability. Its relationship with tumor protein p53 is noted in cancer pathways as p53 can influence beta III tubulin expression impacting cellular proliferation and apoptosis in oncogenic processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Image from Ohmine S et al., Stem Cell Research & Therapy 2011, 2:46, 16 November 2011. Fig 5.; doi: 10.1186/scrt87.Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody (ab41489)
Immunofluorescence analysis of HPC and PBMC blood-derived iPSC clones, staining beta III Tubulin (green) with ab41489.
Cells were fixed with 4% PFA and blocked for 30 minutes in PBS with 5% fetal bovine serum. Cells were incubated with primary antibody overnight at 4°C. A FITC-conjugated donkey anti-chicken IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody (ab41489)
Immunocytochemistry/Immunofluorescence analysis of SpH21 cells in culture labelling beta III Tubulin (green) with ab41489. Cells were fixed with 4% paraformaldehyde. Red - Rabbit anti-GAD67, Blue - DAPI.
Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody (ab41489)
Immunocytochemistry/Immunofluorescence analysis of SpH64 cells in culture labelling beta III Tubulin (green) with ab41489. Cells were fixed with 4% paraformaldehyde. Red - Rabbit anti-GFP, Blue: DAPI.
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