AB76286

Anti-beta III Tubulin (phospho S172) antibody

Name: Anti-beta III Tubulin (phospho S172) antibody (ab76286) | Abcam
Brand: Abcam Limited
SKU: AB76286
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal Beta-3-tubulin phospho S172 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human TUBB3 phospho S172 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

TUBB4, TUBB3, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin (phospho S172) antibody (AB76286)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin (phospho S172) antibody (AB76286)

ab76286, at a 1/100 dilution, staining beta III Tubulin in C2C12 cells by Immunoflurescence.
Image 1 : untreated.
Image 2 : in the presence of the phospho peptide.

Unknown

Western blot - Anti-beta III Tubulin (phospho S172) antibody (AB76286)

All lanes:

Western blot - Anti-beta III Tubulin (phospho S172) antibody (ab76286) at 1/1000 dilution

Lane 1:

Purified human brain tubulin, untreated

Lane 2:

Purified human brain tubulin, treated with ERK2 kinase to phosphorylateSer 172

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TUBB3 phospho S172 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q13509

Specificity

The ab76286 sequence is identical to similar regions in bI, bII, and bIII tubulin isotypes.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab76286 was cross adsorbed to unphosphorylated beta III Tubulin (Ser 172) peptide before affinity purification using phospho beta III Tubulin (Ser 172) peptide (without carrier).

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta III tubulin often referred to as βIII tubulin or beta-tubulin 3 is a microtubule element involved in the cellular cytoskeleton structure. This protein with a molecular mass of approximately 55 kDa plays an important role in the development and maintenance of neuron structures. It is most prominently expressed in neurons within the central and peripheral nervous systems. In these cells beta III tubulin contributes to microtubule polymerization facilitating the formation of the intricate network necessary for cell shape intracellular transport and division.

Biological function summary

Beta III tubulin acts in stabilizing microtubules which are essential for proper neuronal function. It exists as part of the tubulin dimer complex partnering with alpha-tubulin to form the building blocks of microtubules. This particular isotype is often used as a marker for neuronal differentiation due to its specific expression pattern in neural tissues. Its regulation and expression levels are important for neurogenesis and maintaining neuronal plasticity.

Pathways

Beta III tubulin plays a role in neuron-specific intracellular transport and signaling pathways like the Rho GTPase and MAPK signaling pathways. These pathways are involved in cell cycle regulation and apoptotic processes. Relationships with other proteins such as kinesins and dyneins are important in these pathways influencing intracellular transport and signaling through binding and moving along the microtubule tracks created by beta-tubulin isotypes including beta III tubulin.

Abnormal beta III tubulin expression has been associated with cancer particularly in tumors originating from neuronal lineage such as glioblastomas. Overexpression or mutations can contribute to chemoresistance complicating treatment for certain types of cancer. Beta III tubulin is also linked to neurodevelopmental disorders as it affects proper neural network formation and stability. Its relationship with tumor protein p53 is noted in cancer pathways as p53 can influence beta III tubulin expression impacting cellular proliferation and apoptosis in oncogenic processes.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers (PubMed : 34996871, PubMed : 38305685, PubMed : 38609661). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed : 34996871, PubMed : 38305685, PubMed : 38609661). Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed : 34996871, PubMed : 38609661). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed : 20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed : 28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed : 28483977).

See full target information TUBB3 phospho S172

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed38949652

2024

Editing of endogenous tubulins reveals varying effects of tubulin posttranslational modifications on axonal growth and regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ming Lu,Shan Yan,Shih-Chieh Ti,Chaogu Zheng

Cellular and molecular life sciences : CMLS 80:272 PubMed37646890

2023

OSGIN1 is a novel TUBB3 regulator that promotes tumor progression and gefitinib resistance in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomeng Xie,Kyle Vaughn Laster,Jian Li,Wenna Nie,Yong Weon Yi,Kangdong Liu,Yeon-Sun Seong,Zigang Dong,Dong Joon Kim

Bioengineered 13:4112-4121 PubMed35098869

2022

The phosphatase of regenerating liver-3 protein (PRL-3) promotes glioma cell invasiveness by interacting with β3 -tubulin.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenying Ge,Tingxuan Gu,Lingge Zhang,Qingfang Fan,Li Ma,Na Fang

Scientific reports 8:11 PubMed29311646

2018

Generation of Functional Dopaminergic Neurons from Reprogramming Fibroblasts by Nonviral-based Mesoporous Silica Nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Jen-Hsuan Chang,Ping-Hsing Tsai,Kai-Yi Wang,Yu-Ting Wei,Shih-Hwa Chiou,Chung-Yuan Mou

Neuron 90:551-63 PubMed27112495

2016

Phosphorylation of β-Tubulin by the Down Syndrome Kinase, Minibrain/DYRK1a, Regulates Microtubule Dynamics and Dendrite Morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Kassandra M Ori-McKenney,Richard J McKenney,Hector H Huang,Tun Li,Shan Meltzer,Lily Yeh Jan,Ronald D Vale,Arun P Wiita,Yuh Nung Jan

PloS one 9:e94751 PubMed24728101

2014

WISP-1 contributes to fractionated irradiation-induced radioresistance in esophageal carcinoma cell lines and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Feng Li,Li Zhang,Hai-Ying Li,Si-Si Zheng,Liang Zhao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta III Tubulin (phospho S172) antibody

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin (phospho S172) antibody (AB76286)

Western blot - Anti-beta III Tubulin (phospho S172) antibody (AB76286)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise