Rabbit Recombinant Monoclonal TBB4A antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 10 publications.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Mouse | Tested | Expected | Expected | Tested |
Rat | Expected | Expected | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
TUBB4, TUBB5, TUBB4A, TUBB4, TUBB5, Tubulin beta-4A chain, Tubulin 5 beta, Tubulin beta-4 chain
Rabbit Recombinant Monoclonal TBB4A antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 10 publications.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR16775
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
Beta IV Tubulin also known as TUBB4 is an important component of the microtubule structure within cells. It has a molecular mass of approximately 50 kDa. This protein is a critical part of the cytoskeleton and is expressed in a variety of tissues including neuronal tissues. Beta IV Tubulin plays a role in forming dynamic structures that aid in maintaining cell shape facilitating intracellular transport and enabling cell division. Researchers often use beta tubulin staining techniques to observe microtubules in cells which is important for understanding cellular processes.
Beta IV Tubulin contributes significantly to the stability and function of microtubule networks. It is a member of the tubulin family and often forms tubulin heterodimers which are building blocks of microtubules. These microtubules are essential for many cellular activities such as mitosis and transport of organelles. Beta IV Tubulin is integral to the microtubule-based cellular processes and its functions are critical for neuronal development and function because it is a part of the complex cytoskeletal framework.
Beta IV Tubulin is deeply involved in cellular processes such as the MAP kinase pathway and cell cycle regulation. These pathways are pivotal in transmitting signals that regulate cellular processes including growth and differentiation. The protein interacts with other tubulin isotypes and motor proteins like dynein and kinesin contributing to intracellular transport and chromosomal segregation during cell division. These interactions highlight its importance in maintaining proper cellular responses and functions.
Mutations or dysregulation of beta IV Tubulin are associated with neurological conditions like dystonia and Charcot-Marie-Tooth disease. These disorders underline the importance of beta IV Tubulin in neuronal health and development. The protein’s interaction with other tubulins and motor proteins also relates it to neurodegenerative diseases where cytoskeletal integrity and function are often compromised. Understanding these interactions helps unravel the pathogenic mechanisms of these diseases and can guide therapeutic strategies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking/dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution
Lane 1: Human fetal brain lysates at 20 µg
Lane 2: Human cerebellum lysates at 20 µg
Lane 3: Human fetal kidney lysates at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Blocking/dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution
Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 10 µg
Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 10 µg
Lane 3: Neuro-2a (Mouse neuroblastoma cells) whole cell lysates at 10 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Blocking/dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution
Lane 1: Mouse brain lysates at 10 µg
Lane 2: Mouse heart lysates at 10 µg
Lane 3: Mouse kidney lysates at 10 µg
Lane 4: Mouse spleen lysates at 10 µg
Lane 5: Rat brain lysates at 10 µg
Lane 6: Rat heart lysates at 10 µg
Lane 7: Rat kidney lysates at 10 µg
Lane 8: Rat spleen lysates at 10 µg
Lane 9: C6 (Rat glial tumor cells) whole cell lysates at 10 µg
Lane 10: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg
Lane 11: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg
Lane 12: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta IV Tubulin with ab179504 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/200 dilution (green). Cytoplasm staining is observed.
Tubulin is detected with Mouse anti-Tubulin antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). The nuclear counter stain is DAPI (blue).
The negative controls:-
-ve control 1 - ab179504 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.
-ve control 2 - Mouse anti-Tubulin antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex issue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of cerebral cortex.
Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin embedded Mouse hippocampus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of mouse hippocampus.
Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded Rat thalamus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of rat thalamus.
Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling beta IV Tubulinwith ab179504 at 1/20 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line).
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