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AB179504

Anti-beta IV Tubulin antibody [EPR16775]

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(14 Publications)

Anti-beta IV Tubulin antibody [EPR16775] (ab179504) is a rabbit monoclonal antibody detecting beta IV Tubulin in Western Blot, Flow Cytometry (Intra), IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

TUBB4, TUBB5, TUBB4A, Tubulin beta-4A chain, Tubulin 5 beta, Tubulin beta-4 chain

8 Images
Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling beta IV Tubulinwith ab179504 at 1/20 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line).

Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% tritonX-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta IV Tubulin with ab179504 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody (ab150077) at 1/200 dilution (green). Cytoplasm staining is observed.

Tubulin is detected with Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). The nuclear counter stain is DAPI (blue).

The negative controls : -

-ve control 1 - ab179504 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.
-ve control 2 - Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex issue labeling beta IV Tubulin with ab179504 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of cerebral cortex.

Negative control used PBS instead of ab179504 followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)

Immunohistochemical analysis of paraffin embedded Mouse hippocampus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of mouse hippocampus.

Negative control used PBS instead of ab179504 followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)

Immunohistochemical analysis of paraffin-embedded Rat thalamus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of rat thalamus.

Negative control used PBS instead of ab179504 followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)
  • WB

Supplier Data

Western blot - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

Lane 1:

Mouse brain lysates at 10 µg

Lane 2:

Mouse heart lysates at 10 µg

Lane 3:

Mouse kidney lysates at 10 µg

Lane 4:

Mouse spleen lysates at 10 µg

Lane 5:

Rat brain lysates at 10 µg

Lane 6:

Rat heart lysates at 10 µg

Lane 7:

Rat kidney lysates at 10 µg

Lane 8:

Rat spleen lysates at 10 µg

Lane 9:

C6 (Rat glial tumor cells) whole cell lysates at 10 µg

Lane 10:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg

Lane 11:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg

Lane 12:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Western blot - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)
  • WB

Supplier Data

Western blot - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

Lane 1:

Human fetal brain lysates at 20 µg

Lane 2:

Human cerebellum lysates at 20 µg

Lane 3:

Human fetal kidney lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Western blot - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)
  • WB

Supplier Data

Western blot - Anti-beta IV Tubulin antibody [EPR16775] (AB179504)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 10 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 10 µg

Lane 3:

Neuro-2a (Mouse neuroblastoma cells) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

  • Carrier free

    Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-beta IV Tubulin antibody [EPR16775]

  • 578 PE

    PE Anti-beta IV Tubulin antibody [EPR16775]

  • 660 APC

    APC Anti-beta IV Tubulin antibody [EPR16775]

  • HRP

    HRP Anti-beta IV Tubulin antibody [EPR16775]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-beta IV Tubulin antibody [EPR16775]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-beta IV Tubulin antibody [EPR16775]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-beta IV Tubulin antibody [EPR16775]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-beta IV Tubulin antibody [EPR16775]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-beta IV Tubulin antibody [EPR16775]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16775

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-beta IV Tubulin antibody [EPR16775] (ab179504) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of beta IV Tubulin?
Anti-beta IV Tubulin [EPR16775] (ab179504) specifically detects a band for beta IV Tubulin (UniProt: P04350) at a molecular weight of 50kDa.

Trusted by the scientific community
Anti-beta IV Tubulin [EPR16775] (ab179504) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR16775] also available for your convenience: ab179504, Alexa Fluor® 488 - ab204003, Alexa Fluor® 647 - ab204034, Carrier free - ab250103, PE - ab307180, APC - ab307181, HRP - ab307182, Alkaline Phosphatase - ab308805, Alexa Fluor® 594 - ab310535, Alexa Fluor® 555 - ab312066, Alexa Fluor® 568 - ab312543, Alexa Fluor® 750 - ab321223

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta IV Tubulin also known as TUBB4 is an important component of the microtubule structure within cells. It has a molecular mass of approximately 50 kDa. This protein is a critical part of the cytoskeleton and is expressed in a variety of tissues including neuronal tissues. Beta IV Tubulin plays a role in forming dynamic structures that aid in maintaining cell shape facilitating intracellular transport and enabling cell division. Researchers often use beta tubulin staining techniques to observe microtubules in cells which is important for understanding cellular processes.
Biological function summary

Beta IV Tubulin contributes significantly to the stability and function of microtubule networks. It is a member of the tubulin family and often forms tubulin heterodimers which are building blocks of microtubules. These microtubules are essential for many cellular activities such as mitosis and transport of organelles. Beta IV Tubulin is integral to the microtubule-based cellular processes and its functions are critical for neuronal development and function because it is a part of the complex cytoskeletal framework.

Pathways

Beta IV Tubulin is deeply involved in cellular processes such as the MAP kinase pathway and cell cycle regulation. These pathways are pivotal in transmitting signals that regulate cellular processes including growth and differentiation. The protein interacts with other tubulin isotypes and motor proteins like dynein and kinesin contributing to intracellular transport and chromosomal segregation during cell division. These interactions highlight its importance in maintaining proper cellular responses and functions.

Mutations or dysregulation of beta IV Tubulin are associated with neurological conditions like dystonia and Charcot-Marie-Tooth disease. These disorders underline the importance of beta IV Tubulin in neuronal health and development. The protein's interaction with other tubulins and motor proteins also relates it to neurodegenerative diseases where cytoskeletal integrity and function are often compromised. Understanding these interactions helps unravel the pathogenic mechanisms of these diseases and can guide therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
See full target information TUBB4A
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

mBio 16:e0067725 PubMed40521889

2025

Assessment of the public health risk of novel reassortant H3N3 avian influenza viruses that emerged in chickens.

Applications

Unspecified application

Species

Unspecified reactive species

Han Li,Qi Tong,Mengyan Tao,Jixiang Li,Haili Yu,Qiqi Han,Jiancheng Wu,Riguo Lan,Jingjing Han,Haoyu Chang,Yan Li,Juan Pu,Yipeng Sun,Litao Liu,Yajin Qu,Quanlin Li,Lu Lu,Jinhua Liu,Honglei Sun

Nature communications 15:10772 PubMed39738014

2024

Human respiratory organoids sustained reproducible propagation of human rhinovirus C and elucidation of virus-host interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Cun Li,Yifei Yu,Zhixin Wan,Man Chun Chiu,Jingjing Huang,Shuxin Zhang,Xiaoxin Zhu,Qiaoshuai Lan,Yanlin Deng,Ying Zhou,Wei Xue,Ming Yue,Jian-Piao Cai,Cyril Chik-Yan Yip,Kenneth Kak-Yuen Wong,Xiaojuan Liu,Yang Yu,Lin Huang,Hin Chu,Jasper Fuk-Woo Chan,Hans Clevers,Kwok Yung Yuen,Jie Zhou

Cells 12: PubMed36899835

2023

The Protein Network in Subcutaneous Fat Biopsies from Patients with AL Amyloidosis: More Than Diagnosis?

Applications

Unspecified application

Species

Unspecified reactive species

Dario Di Silvestre,Francesca Brambilla,Francesca Lavatelli,Maila Chirivì,Diana Canetti,Claudia Bearzi,Roberto Rizzi,Johan Bijzet,Bouke P C Hazenberg,Vittorio Bellotti,Julian D Gillmore,Pierluigi Mauri

mBio 13:e0148522 PubMed35862773

2022

A Retinol Derivative Inhibits SARS-CoV-2 Infection by Interrupting Spike-Mediated Cellular Entry.

Applications

Unspecified application

Species

Unspecified reactive species

Liangqin Tong,Lin Wang,Shumin Liao,Xiaoping Xiao,Jing Qu,Chunli Wu,Yibin Zhu,Wanbo Tai,Yanhong Huang,Penghua Wang,Liang Li,Renli Zhang,Ye Xiang,Gong Cheng

Nature metabolism 4:547-558 PubMed35534727

2022

A glucose-like metabolite deficient in diabetes inhibits cellular entry of SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Liangqin Tong,Xiaoping Xiao,Min Li,Shisong Fang,Enhao Ma,Xi Yu,Yibin Zhu,Chunli Wu,Deyu Tian,Fan Yang,Jing Sun,Jing Qu,Nianzhen Zheng,Shumin Liao,Wanbo Tai,Shengyong Feng,Liming Zhang,Yuhan Li,Lin Wang,Xuelian Han,Shihui Sun,Long Yang,Hui Zhong,Jincun Zhao,Wenjun Liu,Xiaohui Liu,Penghua Wang,Liang Li,Guangyu Zhao,Renli Zhang,Gong Cheng

Emerging microbes & infections 11:412-423 PubMed34931581

2021

Sequential infections with rhinovirus and influenza modulate the replicative capacity of SARS-CoV-2 in the upper respiratory tract.

Applications

Unspecified application

Species

Unspecified reactive species

Manel Essaidi-Laziosi,Catia Alvarez,Olha Puhach,Pascale Sattonnet-Roche,Giulia Torriani,Caroline Tapparel,Laurent Kaiser,Isabella Eckerle

Cell discovery 7:119 PubMed34903715

2021

SARS-CoV-2 uses metabotropic glutamate receptor subtype 2 as an internalization factor to infect cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jinliang Wang,Guan Yang,Xinxin Wang,Zhiyuan Wen,Lei Shuai,Jie Luo,Chong Wang,Ziruo Sun,Renqiang Liu,Jinying Ge,Xijun He,Ronghong Hua,Xijun Wang,Xiao Yang,Weiye Chen,Gongxun Zhong,Zhigao Bu

Frontiers in cell and developmental biology 9:625251 PubMed33937228

2021

Abnormal Expression of YAP Is Associated With Proliferation, Differentiation, Neutrophil Infiltration, and Adverse Outcome in Patients With Nasal Inverted Papilloma.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Yuan,Rui Zheng,Xiang-Min Zhou,Peng Jin,Zhi-Qun Huang,Xiao-Xue Zi,Qing-Wu Wu,Wei-Hao Wang,Hui-Yi Deng,Wei-Feng Kong,Hui-Jun Qiu,Sui-Zi Zhou,Qian-Min Chen,Yan-Yi Tu,Tao Li,Jing Liu,Kai Sen Tan,Hsiao Hui Ong,Li Shi,Zhuang-Gui Chen,Xue-Kun Huang,Qin-Tai Yang,De-Yun Wang

Nature communications 11:3910 PubMed32764693

2020

Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Na Zhu,Wenling Wang,Zhidong Liu,Chaoyang Liang,Wen Wang,Fei Ye,Baoying Huang,Li Zhao,Huijuan Wang,Weimin Zhou,Yao Deng,Longfei Mao,Chongyu Su,Guangliang Qiang,Taijiao Jiang,Jincun Zhao,Guizhen Wu,Jingdong Song,Wenjie Tan

Cell host & microbe 28:124-133.e4 PubMed32485164

2020

A Mouse Model of SARS-CoV-2 Infection and Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Hui Sun,Qi Chen,Hong-Jing Gu,Guan Yang,Yan-Xiao Wang,Xing-Yao Huang,Su-Su Liu,Na-Na Zhang,Xiao-Feng Li,Rui Xiong,Yan Guo,Yong-Qiang Deng,Wei-Jin Huang,Quan Liu,Quan-Ming Liu,Yue-Lei Shen,Yong Zhou,Xiao Yang,Tong-Yan Zhao,Chang-Fa Fan,Yu-Sen Zhou,Cheng-Feng Qin,You-Chun Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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