AB238433

Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal TBB4A antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

TUBB4, TUBB5, TUBB4A, Tubulin beta-4A chain, Tubulin 5 beta, Tubulin beta-4 chain

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

Immunofluorescent analysis of 100% methanol fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling beta IV Tubulin with ab179509 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Cytoplasm staining on HeLa cell line is observed.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [EPR16776] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows : -
-ve control 1 - ab179509 at 1/500 followed by ab150120 at 1/1000.
-ve control 2. - ab7291 at 1/1000 followed by ab150077 at 1/1000.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179509).

Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell linefrom cervix adenocarcinoma) cell linelabeling beta IV Tubulinwith ab179509 at 1/150 dilution (red) compared with a Rabbit IgG,monoclonal [EPR16776]-Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor^® 488) at 1/500 dilutionwas used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179509).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling beta IV Tubulin with ab179509 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Mouse testis is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary antibody,secondary antibody is ab97051 at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179509).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

Immunofluorescent analysis of 100% methanol fixed C6 (Rat glial tumor cell line ) labeling beta IV Tubulin with ab179509 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution(green).

Cytoplasm staining on C6 cell line is observed.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [EPR16776] -Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 : ab179509 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2. : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179509).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling beta IV Tubulin with ab179509 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Mouse cerebrum is observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody,secondary antibody is ab97051 at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179509).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed C6 (Rat glial tumor cell line) labeling beta IV Tubulin with ab179509 at 1/150 (red) compared with a Rabbit IgG,monoclonal [EPR16776]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor^® 488) at 1/500 dilutionwas used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179509).

Unconjugated

Anti-beta IV Tubulin antibody [EPR16776]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16776

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "IHC application is recommended for mouse only." }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

ab238433 is the carrier-free version of ab179509.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta IV Tubulin also known as TUBB4 is an important component of the microtubule structure within cells. It has a molecular mass of approximately 50 kDa. This protein is a critical part of the cytoskeleton and is expressed in a variety of tissues including neuronal tissues. Beta IV Tubulin plays a role in forming dynamic structures that aid in maintaining cell shape facilitating intracellular transport and enabling cell division. Researchers often use beta tubulin staining techniques to observe microtubules in cells which is important for understanding cellular processes.

Biological function summary

Beta IV Tubulin contributes significantly to the stability and function of microtubule networks. It is a member of the tubulin family and often forms tubulin heterodimers which are building blocks of microtubules. These microtubules are essential for many cellular activities such as mitosis and transport of organelles. Beta IV Tubulin is integral to the microtubule-based cellular processes and its functions are critical for neuronal development and function because it is a part of the complex cytoskeletal framework.

Pathways

Beta IV Tubulin is deeply involved in cellular processes such as the MAP kinase pathway and cell cycle regulation. These pathways are pivotal in transmitting signals that regulate cellular processes including growth and differentiation. The protein interacts with other tubulin isotypes and motor proteins like dynein and kinesin contributing to intracellular transport and chromosomal segregation during cell division. These interactions highlight its importance in maintaining proper cellular responses and functions.

Mutations or dysregulation of beta IV Tubulin are associated with neurological conditions like dystonia and Charcot-Marie-Tooth disease. These disorders underline the importance of beta IV Tubulin in neuronal health and development. The protein’s interaction with other tubulins and motor proteins also relates it to neurodegenerative diseases where cytoskeletal integrity and function are often compromised. Understanding these interactions helps unravel the pathogenic mechanisms of these diseases and can guide therapeutic strategies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB4A

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:1819 PubMed38418467

2024

Dendritic mGluR2 and perisomatic Kv3 signaling regulate dendritic computation of mouse starburst amacrine cells.

Applications

Unspecified application

Species

Unspecified reactive species

Héctor Acarón Ledesma,Jennifer Ding,Swen Oosterboer,Xiaolin Huang,Qiang Chen,Sui Wang,Michael Z Lin,Wei Wei

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16776] - BSA and Azide free (AB238433)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise