AB277630

Anti-beta Synuclein antibody [6A10]

Name: Anti-beta Synuclein antibody [6A10] (ab277630) | Abcam
Brand: Abcam Limited
SKU: AB277630
Price: 515 USD
Availability: InStock

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Mouse Monoclonal beta Synuclein antibody. Suitable for IHC, WB and reacts with Mouse, Rat, Cow samples. Immunogen corresponding to Synthetic Peptide within Human SNCB aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Beta-synuclein, SNCB

2 Images

Supplier Data

Immunohistochemistry - Anti-beta Synuclein antibody [6A10] (AB277630)

Perfusion-fixed, free floating sections of mouse hippocampus section stained for beta Synuclein using ab277630 (green) at 1/500 dilution, and counterstained with rabbit pAb to C-terminal peptide of rat NF-L (red) at 1/5000 dilution in immunohistochemical analysis. The blue is Hoechst staining of nuclear DNA. ab277630 detects protein concentrated in synaptic regions, while the NF-L-ct antibody labels dendrites and axons of neuronal cells.

Supplier Data

Western blot - Anti-beta Synuclein antibody [6A10] (AB277630)

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Western blot - Anti-beta Synuclein antibody [6A10] (ab277630)

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Western blot - Anti-beta Synuclein antibody [6A10] (ab277630) at 1/1000 dilution

Lane 1:

Molecular weight ladder

Lane 2:

rat cortex

Lane 3:

rat cerebellum

Lane 4:

mouse cortex

Lane 5:

mouse cerebellum

Lane 6:

cow cortex

Lane 7:

cow cerebellum

Predicted band size: 14 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6A10

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Cow

Applications

IHC, WB

applications

Immunogen

Synthetic Peptide within Human SNCB aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q16143

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHC-species-checked": "testedAndGuaranteed", "IHC-species-dilution-info": "1/500 - 1/1000", "IHC-species-notes": "transcardial perfusion-fixed with 4% paraformaldehyde for 24 hours, free floating sections", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHC-species-checked": "guaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Cow": { "IHC-species-checked": "guaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from Tissue Culture supernatant.

Storage buffer

Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-synuclein also known as β-synuclein or SNCB is a protein that plays a role in the nervous system. It is a small protein with a molecular weight around 14 kDa. Predominantly beta-synuclein is found in presynaptic terminals of neurons in the central nervous system including the brain and spinal cord. This protein shares significant amino acid sequence identity with alpha-synuclein and gamma-synuclein comprising the synuclein family. It acts as a modulator of neuronal and synaptic function influencing the dynamics of neurotransmitter release.

Biological function summary

Beta-synuclein contributes to cellular homeostasis and synaptic integrity. It is not typically found as part of a larger protein complex like its relative alpha-synuclein which forms toxic aggregates. Instead beta-synuclein exhibits neuroprotective properties helping maintain neuronal health. It possibly regulates synaptic vesicle cycling and mitigates levels of alpha-synuclein aggregation. Research suggests that loss of beta-synuclein function might disrupt synaptic stability leading to neurological issues.

Pathways

Beta-synuclein engages in synaptic function and plasticity. It participates in pathways related to neurotransmitter release and synaptic vesicle trafficking. Beta-synuclein is connected to calcium/calmodulin-dependent protein kinase II (CaMKII) pathways. This interaction supports beta-synuclein's regulatory impact on synaptic vesicle dynamics negatively modulating the aggregation-prone behaviors seen in alpha-synuclein during synucleinopathies.

Beta-synuclein plays a role in neurodegenerative diseases like Parkinson’s disease and dementia with Lewy bodies. Research highlights its relationship with these conditions due to its interaction with alpha-synuclein known to form pathological aggregates and contribute to disease progression. Beta-synuclein's modulation of alpha-synuclein activity can impact the development and progression of these disorders. There is ongoing interest in its potential protective role suggesting it might be leveraged in therapeutic strategies against synucleinopathy-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.

See full target information SNCB

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Synuclein antibody [6A10]

Immunohistochemistry - Anti-beta Synuclein antibody [6A10] (AB277630)

Western blot - Anti-beta Synuclein antibody [6A10] (AB277630)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise