AB6165

Anti-beta Synuclein antibody

Name: Anti-beta Synuclein antibody (ab6165) | Abcam
Brand: Abcam Limited
SKU: AB6165
Price: 545 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal beta Synuclein antibody. Suitable for IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human SNCB aa 100 to C-terminus.

View Alternative Names

Beta-synuclein, SNCB

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (AB6165)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (AB6165)

ab6165 staining beta synuclein in human occipital cortex.
Left panel : with primary antibody diluted 1 : 4000. Right panel : isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature : sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SNCB aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16143

Specificity

Less than 0.1% cross reactivity against human alpha-synuclein Cross reactivity: This antibody is known to react with human and rat. Not yet tested in other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

pH: 7 - 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-synuclein also known as β-synuclein or SNCB is a protein that plays a role in the nervous system. It is a small protein with a molecular weight around 14 kDa. Predominantly beta-synuclein is found in presynaptic terminals of neurons in the central nervous system including the brain and spinal cord. This protein shares significant amino acid sequence identity with alpha-synuclein and gamma-synuclein comprising the synuclein family. It acts as a modulator of neuronal and synaptic function influencing the dynamics of neurotransmitter release.

Biological function summary

Beta-synuclein contributes to cellular homeostasis and synaptic integrity. It is not typically found as part of a larger protein complex like its relative alpha-synuclein which forms toxic aggregates. Instead beta-synuclein exhibits neuroprotective properties helping maintain neuronal health. It possibly regulates synaptic vesicle cycling and mitigates levels of alpha-synuclein aggregation. Research suggests that loss of beta-synuclein function might disrupt synaptic stability leading to neurological issues.

Pathways

Beta-synuclein engages in synaptic function and plasticity. It participates in pathways related to neurotransmitter release and synaptic vesicle trafficking. Beta-synuclein is connected to calcium/calmodulin-dependent protein kinase II (CaMKII) pathways. This interaction supports beta-synuclein's regulatory impact on synaptic vesicle dynamics negatively modulating the aggregation-prone behaviors seen in alpha-synuclein during synucleinopathies.

Beta-synuclein plays a role in neurodegenerative diseases like Parkinson’s disease and dementia with Lewy bodies. Research highlights its relationship with these conditions due to its interaction with alpha-synuclein known to form pathological aggregates and contribute to disease progression. Beta-synuclein's modulation of alpha-synuclein activity can impact the development and progression of these disorders. There is ongoing interest in its potential protective role suggesting it might be leveraged in therapeutic strategies against synucleinopathy-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.

See full target information SNCB

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 10:1 PubMed38167744

2024

Aggregate-prone brain regions in Parkinson's disease are rich in unique N-terminus α-synuclein conformers with high proteolysis susceptibility.

Applications

Unspecified application

Species

Unspecified reactive species

James A Wiseman,Helen C Murray,Richard L M F Faull,Michael Dragunow,Clinton P Turner,Birger Victor Dieriks,Maurice A Curtis

Cells 12: PubMed36831238

2023

Interneuronal In Vivo Transfer of Synaptic Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Klinkenberg,Michael Helwig,Rita Pinto-Costa,Angela Rollar,Raffaella Rusconi,Donato A Di Monte,Ayse Ulusoy

Disease models & mechanisms 10:619-631 PubMed28108469

2017

Blood RNA biomarkers in prodromal PARK4 and rapid eye movement sleep behavior disorder show role of complexin 1 loss for risk of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Suna Lahut,Suzana Gispert,Özgür Ömür,Candan Depboylu,Kay Seidel,Jorge Antolio Domínguez-Bautista,Nadine Brehm,Hülya Tireli,Karl Hackmann,Caroline Pirkevi,Barbara Leube,Vincent Ries,Kerstin Reim,Nils Brose,Wilfred F den Dunnen,Madrid Johnson,Zsuzsanna Wolf,Marc Schindewolf,Wiebke Schrempf,Kathrin Reetz,Peter Young,David Vadasz,Achilleas S Frangakis,Evelin Schröck,Helmuth Steinmetz,Marina Jendrach,Udo Rüb,Ayşe Nazlı Başak,Wolfgang Oertel,Georg Auburger

Journal of neuroscience research 94:717-35 PubMed27265751

2016

LRRK2 knockdown in zebrafish causes developmental defects, neuronal loss, and synuclein aggregation.

Applications

Unspecified application

Species

Unspecified reactive species

Shubhangi Prabhudesai,Fatima Zahra Bensabeur,Rashed Abdullah,Indranil Basak,Solange Baez,Guido Alves,Nathalia G Holtzman,Jan Petter Larsen,Simon Geir Møller

Brain : a journal of neurology 139:856-70 PubMed26719384

2016

Brain propagation of transduced α-synuclein involves non-fibrillar protein species and is enhanced in α-synuclein null mice.

Applications

Species

Mouse

Michael Helwig,Michael Klinkenberg,Raffaella Rusconi,Ruth E Musgrove,Nour K Majbour,Omar M A El-Agnaf,Ayse Ulusoy,Donato A Di Monte

Neuropathology and applied neurobiology 38:737-43 PubMed22686686

2012

Pick's pathology in Parkinson's disease with dementia.

Applications

Unspecified application

Species

Unspecified reactive species

D Vilas,M J Marti,T Botta-Orfila,M Colom-Cadena,E Gelpi

Brain : a journal of neurology 133:3724-33 PubMed20959308

2010

The decrease of β-synuclein in cortical brain areas defines a molecular subgroup of dementia with Lewy bodies.

Applications

Species

Human

Katrin Beyer,Montserrat Domingo-Sàbat,Cristina Santos,Eduardo Tolosa,Isidro Ferrer,Aurelio Ariza

The Journal of biological chemistry 276:45320-9 PubMed11560921

2001

alpha-Synuclein affects the MAPK pathway and accelerates cell death.

Applications

Species

Human

A Iwata,M Maruyama,I Kanazawa,N Nukina

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-beta Synuclein antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (AB6165)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Primary Antibodies

Complementary Products

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise