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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Monoclonal beta Synuclein antibody. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human, Recombinant fragment - Human samples. Cited in 12 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Tested | Expected | Tested | Tested | Expected |
Mouse | Tested | Expected | Expected | Tested | Expected |
Rat | Tested | Tested | Expected | Tested | Tested |
Recombinant fragment - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 | Notes For unpurified use at 1/100-1/250 dilution Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/50 | Notes For unpurified use at 1/100-1/250 dilution Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/50 | Notes For unpurified use at 1/100-1/250 dilution Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/100 | Notes For unpurified use at 1/100-1/250 dilution. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20 | Notes For unpurified use at 1/40 |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
Species Mouse | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
Species Rat | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
Species Human | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.
Beta-synuclein, SNCB
Rabbit Monoclonal beta Synuclein antibody. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human, Recombinant fragment - Human samples. Cited in 12 publications.
Beta-synuclein, SNCB
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EP1537Y
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Beta-synuclein contributes to cellular homeostasis and synaptic integrity. It is not typically found as part of a larger protein complex like its relative alpha-synuclein which forms toxic aggregates. Instead beta-synuclein exhibits neuroprotective properties helping maintain neuronal health. It possibly regulates synaptic vesicle cycling and mitigates levels of alpha-synuclein aggregation. Research suggests that loss of beta-synuclein function might disrupt synaptic stability leading to neurological issues.
Beta-synuclein also known as β-synuclein or SNCB is a protein that plays a role in the nervous system. It is a small protein with a molecular weight around 14 kDa. Predominantly beta-synuclein is found in presynaptic terminals of neurons in the central nervous system including the brain and spinal cord. This protein shares significant amino acid sequence identity with alpha-synuclein and gamma-synuclein comprising the synuclein family. It acts as a modulator of neuronal and synaptic function influencing the dynamics of neurotransmitter release.
Beta-synuclein engages in synaptic function and plasticity. It participates in pathways related to neurotransmitter release and synaptic vesicle trafficking. Beta-synuclein is connected to calcium/calmodulin-dependent protein kinase II (CaMKII) pathways. This interaction supports beta-synuclein's regulatory impact on synaptic vesicle dynamics negatively modulating the aggregation-prone behaviors seen in alpha-synuclein during synucleinopathies.
Beta-synuclein plays a role in neurodegenerative diseases like Parkinson’s disease and dementia with Lewy bodies. Research highlights its relationship with these conditions due to its interaction with alpha-synuclein known to form pathological aggregates and contribute to disease progression. Beta-synuclein's modulation of alpha-synuclein activity can impact the development and progression of these disorders. There is ongoing interest in its potential protective role suggesting it might be leveraged in therapeutic strategies against synucleinopathy-related diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Purified ab76111 at 1/20 dilution (0.6 µg) immunoprecipitating beta Synuclein in Human brain lysate.
Lane 1 (input): Human brain lysate 10 µg
Lane 2 (+): ab76111 + Human brain lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76111 in Human brain lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 18 kDa
All lanes: Immunoprecipitation - Anti-beta Synuclein antibody [EP1537Y] (AB76111)
Predicted band size: 14 kDa
All lanes: Western blot - Anti-beta Synuclein antibody [EP1537Y] (AB76111) at 1/10000 dilution
Lane 1: Human brain lysate at 20 µg
Lane 2: Mouse brain lysate at 20 µg
Lane 3: Rat brain lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa
Intracellular Flow Cytometry analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling beta Synuclein with purified ab76111 at 1/100 dilution (10μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling beta Synuclein with purified ab76111 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling beta Synuclein with purified ab76111 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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