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AB76111

Anti-beta Synuclein antibody [EP1537Y]

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(15 Publications)

Rabbit Monoclonal beta Synuclein antibody. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human, Recombinant fragment - Human samples. Cited in 15 publications.

View Alternative Names

Beta-synuclein, SNCB

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (AB76111)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (AB76111)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling beta Synuclein with purified ab76111 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunoprecipitation - Anti-beta Synuclein antibody [EP1537Y] (AB76111)
  • IP

Unknown

Immunoprecipitation - Anti-beta Synuclein antibody [EP1537Y] (AB76111)

Purified ab76111 at 1/20 dilution (0.6 µg) immunoprecipitating beta Synuclein in Human brain lysate.
Lane 1 (input) : Human brain lysate 10 µg
Lane 2 (+) : ab76111 + Human brain lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab76111 in Human brain lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 18 kDa

All lanes:

Immunoprecipitation - Anti-beta Synuclein antibody [EP1537Y] (ab76111)

Predicted band size: 14 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-beta Synuclein antibody [EP1537Y] (AB76111)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta Synuclein antibody [EP1537Y] (AB76111)

Immunocytochemistry/ Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling beta Synuclein with purified ab76111 at 1/50 dilution (2.4 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (AB76111)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (AB76111)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling beta Synuclein with purified ab76111 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cytometry (Intracellular) - Anti-beta Synuclein antibody [EP1537Y] (AB76111)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-beta Synuclein antibody [EP1537Y] (AB76111)

Intracellular Flow Cytometry analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling beta Synuclein with purified ab76111 at 1/100 dilution (10μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (AB76111)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (AB76111)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling beta Synuclein with purified ab76111 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Western blot - Anti-beta Synuclein antibody [EP1537Y] (AB76111)
  • WB

Unknown

Western blot - Anti-beta Synuclein antibody [EP1537Y] (AB76111)

All lanes:

Western blot - Anti-beta Synuclein antibody [EP1537Y] (ab76111) at 1/10000 dilution

Lane 1:

Human brain lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 14 kDa

Observed band size: 18 kDa

false

Western blot - Anti-beta Synuclein antibody [EP1537Y] (AB76111)
  • WB

Unknown

Western blot - Anti-beta Synuclein antibody [EP1537Y] (AB76111)

A : Anti-beta Synuclein antibody [EP1537Y] (ab76111)

B : Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

All lanes:

Western blot - Anti-beta Synuclein antibody [EP1537Y] (ab76111) at 1/10000 dilution

Lane 1:

His-Tagged human beta Synuclein (aa 1 to 134) recombinant protein at 0.015 µg

Lane 2:

His-Tagged alpha synuclein (aa 1 to 140) recombinant protein at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 14 kDa

Observed band size: 18 kDa

false

  • HRP

    HRP Anti-beta Synuclein antibody [EP1537Y]

  • Carrier free

    Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1537Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, Flow Cyt (Intra), WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB6165

Anti-beta Synuclein antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (AB6165)

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Complementary Products

Assay Kits

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Primary Antibodies

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Primary Antibodies

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Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] - BSA and Azide free (AB315443)

  • 0
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Primary Antibodies

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-synuclein also known as β-synuclein or SNCB is a protein that plays a role in the nervous system. It is a small protein with a molecular weight around 14 kDa. Predominantly beta-synuclein is found in presynaptic terminals of neurons in the central nervous system including the brain and spinal cord. This protein shares significant amino acid sequence identity with alpha-synuclein and gamma-synuclein comprising the synuclein family. It acts as a modulator of neuronal and synaptic function influencing the dynamics of neurotransmitter release.
Biological function summary

Beta-synuclein contributes to cellular homeostasis and synaptic integrity. It is not typically found as part of a larger protein complex like its relative alpha-synuclein which forms toxic aggregates. Instead beta-synuclein exhibits neuroprotective properties helping maintain neuronal health. It possibly regulates synaptic vesicle cycling and mitigates levels of alpha-synuclein aggregation. Research suggests that loss of beta-synuclein function might disrupt synaptic stability leading to neurological issues.

Pathways

Beta-synuclein engages in synaptic function and plasticity. It participates in pathways related to neurotransmitter release and synaptic vesicle trafficking. Beta-synuclein is connected to calcium/calmodulin-dependent protein kinase II (CaMKII) pathways. This interaction supports beta-synuclein's regulatory impact on synaptic vesicle dynamics negatively modulating the aggregation-prone behaviors seen in alpha-synuclein during synucleinopathies.

Beta-synuclein plays a role in neurodegenerative diseases like Parkinson’s disease and dementia with Lewy bodies. Research highlights its relationship with these conditions due to its interaction with alpha-synuclein known to form pathological aggregates and contribute to disease progression. Beta-synuclein's modulation of alpha-synuclein activity can impact the development and progression of these disorders. There is ongoing interest in its potential protective role suggesting it might be leveraged in therapeutic strategies against synucleinopathy-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.
See full target information SNCB
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 6:103945 PubMed40700012

2025

Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Leonardo A Parra-Rivas,Rohan Sharma,Trinity E Rust,Hannah O Bazick,Jared Carlson-Stevermer,Mark J Zylka,Yuki Ogawa,Subhojit Roy

International journal of medical sciences 21:1472-1490 PubMed38903914

2024

Retinal ganglion cell type-specific expression of synuclein family members revealed by scRNA-sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Qingwen Yang,Lin Liu,Fang He,Wenna Zhao,Zhongqun Chen,Xiaotian Wu,Bilin Rao,Xin Lin,Fangyuan Mao,Jia Qu,Jun Zhang

Neuron 111:4006-4023.e10 PubMed38128479

2023

Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function.

Applications

Unspecified application

Species

Unspecified reactive species

Leonardo A Parra-Rivas,Kayalvizhi Madhivanan,Brent D Aulston,Lina Wang,Dube Dheeraj Prakashchand,Nicholas P Boyer,Veronica M Saia-Cereda,Kristen Branes-Guerrero,Donald P Pizzo,Pritha Bagchi,V S Sundar,Yong Tang,Utpal Das,David A Scott,Padmini Rangamani,Yuki Ogawa, Subhojit Roy

Biomolecules 13: PubMed36671565

2023

Age-Related Changes of the Synucleins Profile in the Mouse Retina.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Batista Dias,Luísa de Lemos,Luís Sousa,Diogo B Bitoque,Gabriela Araújo Silva,Miguel C Seabra,Sandra Tenreiro

Acta neuropathologica communications 10:180 PubMed36517890

2022

SNCA genetic lowering reveals differential cognitive function of alpha-synuclein dependent on sex.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer L Brown,Damyan W Hart,Gabriel E Boyle,Taylor G Brown,Michael LaCroix,Andrés M Baraibar,Ross Pelzel,Minwoo Kim,Mathew A Sherman,Samuel Boes,Michelle Sung,Tracy Cole,Michael K Lee,Alfonso Araque,Sylvain E Lesné

FASEB journal : official publication of the Federa 33:10240-10256 PubMed31211923

2019

Extracellular α-synuclein enters dopaminergic cells by modulating flotillin-1-assisted dopamine transporter endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junpei Kobayashi,Takafumi Hasegawa,Naoto Sugeno,Shun Yoshida,Tetsuya Akiyama,Koki Fujimori,Hiroyasu Hatakeyama,Yasuo Miki,Arata Tomiyama,Yasushi Kawata,Mitsunori Fukuda,Ichiro Kawahata,Tohru Yamakuni,Michinori Ezura,Akio Kikuchi,Toru Baba,Atsushi Takeda,Makoto Kanzaki,Koichi Wakabayashi,Hideyuki Okano,Masashi Aoki

Scientific reports 8:17563 PubMed30510219

2018

Investigating the neuroprotective effect of AAV-mediated β-synuclein overexpression in a transgenic model of synucleinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Dorian Sargent,Dominique Bétemps,Matthieu Drouyer,Jérémy Verchere,Damien Gaillard,Jean-Noël Arsac,Latifa Lakhdar,Anna Salvetti,Thierry Baron

Methods in molecular biology (Clifton, N.J.) 1854:1-12 PubMed29936691

2018

Visualization and Measurement of Multiple Components of the Autophagy Flux.

Applications

Unspecified application

Species

Unspecified reactive species

Tracey Evans,Robert Button,Oleg Anichtchik,Shouqing Luo

Frontiers in molecular neuroscience 11:94 PubMed29636661

2018

Sumoylation Protects Against β-Synuclein Toxicity in Yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Blagovesta Popova,Alexandra Kleinknecht,Patricia Arendarski,Jasmin Mischke,Dan Wang,Gerhard H Braus

Frontiers in molecular neuroscience 11:36 PubMed29497361

2018

Silencing Alpha Synuclein in Mature Nigral Neurons Results in Rapid Neuroinflammation and Subsequent Toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Benskey,Rhyomi C Sellnow,Ivette M Sandoval,Caryl E Sortwell,Jack W Lipton,Fredric P Manfredsson
View all publications
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