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Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting beta Synuclein in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.



- BSA, sodium azide, and glycerol-free for easy conjugation

- Biophysical QC for unrivalled batch-batch consistency


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Images

Immunoprecipitation - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (AB221908), expandable thumbnail
  • Western blot - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (AB221908), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (AB221908), expandable thumbnail
  • Western blot - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (AB221908), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (AB221908), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Expected
Tested
Tested
Expected
Mouse
Tested
Expected
Expected
Tested
Expected
Rat
Tested
Tested
Expected
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.

Alternative names

Beta-synuclein, SNCB

Recommended products

Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting beta Synuclein in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.



- BSA, sodium azide, and glycerol-free for easy conjugation

- Biophysical QC for unrivalled batch-batch consistency


Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP1537Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

What is this antibody validated in?


Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of beta Synuclein?


Anti-beta Synuclein [EP1537Y] - BSA and Azide free (ab221908) specifically detects a band for beta Synuclein (UniProt: Q16143) at a molecular weight of 14kDa.

Other related products


We have a range of other formats of antibody clone [EP1537Y] also available for your convenience:
Anti-beta Synuclein antibody [EP1537Y] ab76111, HRP - HRP Anti-beta Synuclein antibody [EP1537Y] ab215783, Carrier free - ab221908



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beta-synuclein also known as Β-synuclein or SNCB is a protein that plays a role in the nervous system. It is a small protein with a molecular weight around 14 kDa. Predominantly beta-synuclein is found in presynaptic terminals of neurons in the central nervous system including the brain and spinal cord. This protein shares significant amino acid sequence identity with alpha-synuclein and gamma-synuclein comprising the synuclein family. It acts as a modulator of neuronal and synaptic function influencing the dynamics of neurotransmitter release.

Biological function summary

Beta-synuclein contributes to cellular homeostasis and synaptic integrity. It is not typically found as part of a larger protein complex like its relative alpha-synuclein which forms toxic aggregates. Instead beta-synuclein exhibits neuroprotective properties helping maintain neuronal health. It possibly regulates synaptic vesicle cycling and mitigates levels of alpha-synuclein aggregation. Research suggests that loss of beta-synuclein function might disrupt synaptic stability leading to neurological issues.

Pathways

Beta-synuclein engages in synaptic function and plasticity. It participates in pathways related to neurotransmitter release and synaptic vesicle trafficking. Beta-synuclein is connected to calcium/calmodulin-dependent protein kinase II (CaMKII) pathways. This interaction supports beta-synuclein's regulatory impact on synaptic vesicle dynamics negatively modulating the aggregation-prone behaviors seen in alpha-synuclein during synucleinopathies.

Associated diseases and disorders

Beta-synuclein plays a role in neurodegenerative diseases like Parkinson’s disease and dementia with Lewy bodies. Research highlights its relationship with these conditions due to its interaction with alpha-synuclein known to form pathological aggregates and contribute to disease progression. Beta-synuclein's modulation of alpha-synuclein activity can impact the development and progression of these disorders. There is ongoing interest in its potential protective role suggesting it might be leveraged in therapeutic strategies against synucleinopathy-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

  • Immunoprecipitation - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Immunoprecipitation - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    Purified Anti-beta Synuclein antibody [EP1537Y] ab76111 at 1/20 dilution (0.6 µg) immunoprecipitating beta Synuclein in Human brain lysate.
    Lane 1 (input): Human brain lysate 10 µg
    Lane 2 (+): Anti-beta Synuclein antibody [EP1537Y] ab76111 + Human brain lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-beta Synuclein antibody [EP1537Y] ab76111 in Human brain lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 18 kDa
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta Synuclein antibody [EP1537Y] ab76111).

    All lanes: Immunoprecipitation - Anti-beta Synuclein antibody [EP1537Y] (Anti-beta Synuclein antibody [EP1537Y] ab76111)

    Predicted band size: 14 kDa

  • Western blot - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Western blot - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta Synuclein antibody [EP1537Y] ab76111).

    All lanes: Western blot - Anti-beta Synuclein antibody [EP1537Y] (Anti-beta Synuclein antibody [EP1537Y] ab76111) at 1/10000 dilution

    Lane 1: Human brain lysate at 20 µg

    Lane 2: Mouse brain lysate at 20 µg

    Lane 3: Rat brain lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 14 kDa

    Observed band size: 18 kDa

  • Flow Cytometry (Intracellular) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    Intracellular Flow Cytometry analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling beta Synuclein with purified Anti-beta Synuclein antibody [EP1537Y] ab76111 at 1/100 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta Synuclein antibody [EP1537Y] ab76111).

  • Western blot - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Western blot - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    A: Anti-beta Synuclein antibody [EP1537Y] (Anti-beta Synuclein antibody [EP1537Y] ab76111)

    B: Anti-Alpha-synuclein antibody [MJFR1] (Anti-Alpha-synuclein antibody [MJFR1] ab138501)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta Synuclein antibody [EP1537Y] ab76111).

    All lanes: Western blot - Anti-beta Synuclein antibody [EP1537Y] (Anti-beta Synuclein antibody [EP1537Y] ab76111) at 1/10000 dilution

    Lane 1: His-Tagged human beta Synuclein (aa 1 to 134) recombinant protein at 0.015 µg

    Lane 2: His-Tagged alpha synuclein (aa 1 to 140) recombinant protein at 0.015 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa

    Observed band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling beta Synuclein with purified Anti-beta Synuclein antibody [EP1537Y] ab76111 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta Synuclein antibody [EP1537Y] ab76111).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling beta Synuclein with purified Anti-beta Synuclein antibody [EP1537Y] ab76111 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta Synuclein antibody [EP1537Y] ab76111).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling beta Synuclein with purified Anti-beta Synuclein antibody [EP1537Y] ab76111 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta Synuclein antibody [EP1537Y] ab76111)

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    Immunocytochemistry/ Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling beta Synuclein with purified Anti-beta Synuclein antibody [EP1537Y] ab76111 at 1/50 dilution (2.4 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta Synuclein antibody [EP1537Y] ab76111).

  • Flow Cytometry (Intracellular) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    PC-12 cells were fixed with 100?l 4% PFA for 10 min at RT, then permeabilized with 100?l 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate ab221908 for 30 min at room temperature (RT) and then incubate Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) for 30 min at room temperature.

  • Western blot - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Western blot - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lane 1: His-Tagged human beta Synuclein (aa 1 to 134) recombinant protein at 15 µg

    Lane 2: His-Tagged human alpha synuclein (aa 1 to 140) recombinant protein at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling beta Synuclein with ab221908 at 1/5000 dilution. followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling beta Synuclein with ab221908 at 1/5000 dilution. followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling beta Synuclein with ab221908 at 1/5000 dilution. followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling beta Synuclein with ab221908 at 1/100 dilution, followed by AlexaFluor® 488-conjugated Goat anti-Rabbit IgG H&L preadsorbed secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution at RT for 45 min (Green). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab221908 overnight at 4° C. Nucleus were visualized using DAPI.

  • Immunoprecipitation - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908), expandable thumbnail

    Immunoprecipitation - Anti-beta Synuclein antibody [EP1537Y] - BSA and Azide free (ab221908)

    beta Synuclein was immunoprecipitated from 0.35 mg of mouse brain tissue lysate with ab221908 at 1/30 dilution. Western blot was performed from the immunoprecipitated using ab221908 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 ?g.
    Lane 2: Mouse brain tissue lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab221908 in HepG2 whole cell lysate.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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