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AB324049

Anti-Beta TRCP/HOS antibody [EPR25381-38]

  • KO Validated
  • RabMAb
  • Recombinant
  • 20ul selling size
  • What is this?

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Rabbit Recombinant Monoclonal Beta TRCP/HOS antibody. Suitable for WB and reacts with Mouse, Human samples.

View Alternative Names

BTRCP, FBW1A, FBXW1A, BTRC, F-box/WD repeat-containing protein 1A, E3RSIkappaB, Epididymis tissue protein Li 2a, F-box and WD repeats protein beta-TrCP, pIkappaBalpha-E3 receptor subunit, Beta TRCP/HOS

3 Images
Western blot - Anti-Beta TRCP/HOS antibody [EPR25381-38] (AB324049)
  • WB

Supplier Data

Western blot - Anti-Beta TRCP/HOS antibody [EPR25381-38] (AB324049)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : B16-F10.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Beta TRCP/HOS antibody [EPR25381-38] (ab324049) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 80 µg

Lane 2:

B16-F10 (mouse skin melanoma cell) whole cell lysate at 80 µg

Lane 3:

RD (human muscle multinucleated spindle-shaped cell) whole cell lysate at 80 µg

Lane 4:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Beta TRCP/HOS antibody [EPR25381-38] (AB324049)
  • WB

Supplier Data

Western blot - Anti-Beta TRCP/HOS antibody [EPR25381-38] (AB324049)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab324049 was shown to bind specifically to Beta TRCP/HOS. Target of interest was observed at 60 kDa in wild-type HEK-293 cell lysates (lane 1) with no signal observed at this size in BTRC knockout cell line (lane 2) (knockout cell line ab266201 / knockout cell lysate ab257864).

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible. When lysates were stored at -80℃ prior to Western Blotting degradation products were observed.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Beta TRCP/HOS antibody [EPR25381-38] (ab324049) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

BTRC knockout HEK-293 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Beta TRCP/HOS antibody [EPR25381-38] (AB324049)
  • WB

Supplier Data

Western blot - Anti-Beta TRCP/HOS antibody [EPR25381-38] (AB324049)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible. When lysates were stored at -80℃ prior to Western Blotting degradation products were observed.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Beta TRCP/HOS antibody [EPR25381-38] (ab324049) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

mIMCD3 (mouse inner medullary collecting duct epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa,36 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25381-38

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 10066435, PubMed : 10497169, PubMed : 10644755, PubMed : 10835356, PubMed : 11158290, PubMed : 11238952, PubMed : 11359933, PubMed : 11994270, PubMed : 12791267, PubMed : 12902344, PubMed : 14603323, PubMed : 14681206, PubMed : 14988407, PubMed : 15448698, PubMed : 15917222, PubMed : 16371461, PubMed : 22017875, PubMed : 22017876, PubMed : 22017877, PubMed : 22087322, PubMed : 25503564, PubMed : 25704143, PubMed : 36608670, PubMed : 9859996, PubMed : 9990852). Recognizes and binds to phosphorylated target proteins (PubMed : 10066435, PubMed : 10497169, PubMed : 10644755, PubMed : 10835356, PubMed : 11158290, PubMed : 11238952, PubMed : 11359933, PubMed : 11994270, PubMed : 12791267, PubMed : 12902344, PubMed : 14603323, PubMed : 14681206, PubMed : 14988407, PubMed : 15448698, PubMed : 15917222, PubMed : 16371461, PubMed : 22017875, PubMed : 22017876, PubMed : 22017877, PubMed : 22087322, PubMed : 25503564, PubMed : 25704143, PubMed : 36608670, PubMed : 9859996, PubMed : 9990852). SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling (PubMed : 12077367, PubMed : 12820959). SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1, ATF4, CDC25A, DLG1, FBXO5, PER1, SMAD3, SMAD4, SNAI1 and probably NFKB2 (PubMed : 10835356, PubMed : 11238952, PubMed : 14603323, PubMed : 14681206). SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription (PubMed : 10066435, PubMed : 10497169, PubMed : 10644755, PubMed : 9859996). Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22' (PubMed : 10066435). The SCF(FBXW11) complex also regulates NF-kappa-B by mediating ubiquitination of phosphorylated NFKB1 : specifically ubiquitinates the p105 form of NFKB1, leading to its degradation (PubMed : 10835356, PubMed : 11158290, PubMed : 14673179). SCF(BTRC) mediates the ubiquitination of CEP68; this is required for centriole separation during mitosis (PubMed : 25503564, PubMed : 25704143). SCF(BTRC) mediates the ubiquitination and subsequent degradation of nuclear NFE2L1 (By similarity). Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and PER2 (PubMed : 15917222). May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3 (PubMed : 16371461). Mediates ubiquitination of REST, thereby leading to its proteasomal degradation (PubMed : 18354482, PubMed : 21258371). SCF(BTRC) mediates the ubiquitination and subsequent proteasomal degradation of KLF4; thereby negatively regulating cell pluripotency maintenance and embryogenesis (By similarity). SCF(BTRC) acts as a regulator of mTORC1 signaling pathway by catalyzing ubiquitination and subsequent proteasomal degradation of phosphorylated DEPTOR, TFE3 and MITF (PubMed : 22017875, PubMed : 22017876, PubMed : 22017877, PubMed : 33110214, PubMed : 36608670). SCF(BTRC) directs 'Lys-48'-linked ubiquitination of UBR2 in the T-cell receptor signaling pathway (PubMed : 38225265).
See full target information BTRC
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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