AB315211

Anti-beta Tubulin antibody [1B12] - Loading Control

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Mouse Monoclonal TBB antibody. Suitable for WB, ICC/IF and reacts with Human, Rat, Mouse samples. Immunogen corresponding to Native Full Length Protein corresponding to Pig TBB_PIG.

View Alternative Names

Tubulin beta chain, Beta-tubulin

2 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [1B12] - Loading Control (AB315211)

Immunofluorescent analysis of HeLa cells stained with ab315211, dilution 1 : 5,000 in green, and costained with chicken pAb to the nucleic acid binding protein FOX2, CPCA-FOX2, dilution 1 : 1,000 in red. Blue is DAPI staining of nuclear DNA. ab315211 antibody produces strong staining of microtubules in the cytoplasm, while the CPCA-FOX2 antibody labels the nuclei of HeLa cells. Mouse select each image for larger view.

Supplier Data

Western blot - Anti-beta Tubulin antibody [1B12] - Loading Control (AB315211)

Western blot analysis of whole tissue or cell lysates using ab315211, dilution 1 : 10,000 in green : [1] protein standard (red), [2] adult rat brain, [3] adult mouse brain, [4] NIH-3T3 cells, [5] HEK293 cells, [6] HeLa cells, [7] SH-SY5Y cells. The strong clean band with apparent SDS-PAGE molecular weight of ~50kDa corresponds to the β-tubulin proteins

All lanes:

Western blot - Anti-beta Tubulin antibody [1B12] - Loading Control (ab315211) at 1/10000 dilution

Lane 2:

Adult rat brain

Lane 3:

Adult mouse brain

Lane 4:

NIH-3T3 cell lysate

Lane 5:

HEK293 cell lysate

Lane 6:

HeLa cell lysate

Lane 7:

SH-SY5Y cell lysate

Observed band size: 50 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1B12

Isotype

IgG2b

Carrier free

Reacts with

Mouse, Human, Rat

Applications

ICC/IF, WB

applications

Immunogen

Native Full Length Protein corresponding to Pig TBB_PIG.

P02554

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7 - 8 Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed : 7225365). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed : 7225365). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed : 7225365).

See full target information TBB_PIG

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Tubulin antibody [1B12] - Loading Control

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [1B12] - Loading Control (AB315211)

Western blot - Anti-beta Tubulin antibody [1B12] - Loading Control (AB315211)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise