AB131205

Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control

Name: Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (ab131205) | Abcam
Brand: Abcam Limited
SKU: AB131205
Price: 400 USD
Availability: InStock
Rating: 5 (6 reviews)

Lab Essentials
What is this?

(6 Reviews)

(30 Publications)

Mouse Monoclonal Beta-5-tubulin antibody. Microtubule marker. Suitable for WB, ICC/IF and reacts with Rat, African green monkey, Human, Mouse samples. Cited in 30 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TUBB.

View Alternative Names

TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain

5 Images

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (AB131205)

Immunocytochemistry stain of Hela using ab131205(1 : 300). This image was produced with antibody produced in ascites.

Unknown

Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (AB131205)

ab131205 stability test under 37°C for 50 hours compared to 4°C for 50 hours. This image was produced with antibody produced in ascites.

All lanes:

Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (ab131205)

Predicted band size: 49 kDa

false

Supplier Data

Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (AB131205)

This image was produced with antibody produced in ascites.

All lanes:

Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (ab131205) at 1/500 dilution

Lane 1:

COS7 cell lysate

Lane 2:

LNCap cell lysate

Lane 3:

MCF7 cell lysate

Lane 4:

C2C12 cell lysate

Predicted band size: 49 kDa

false

Supplier Data

Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (AB131205)

All lanes:

Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (ab131205) at 1/5000 dilution

Lane 1:

C6 Cell lysate

Lane 2:

K562 Cell lysate

Lane 3:

COS7 Cell lysate

Lane 4:

3T3 Cell lysate

Lane 5:

HeLa Cell lysate

Predicted band size: 49 kDa

false

Unknown

Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (AB131205)

This image was produced with antibody produced in ascites.

All lanes:

Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (ab131205) at 1/1000 dilution

Lane 1:

A431 cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

Jurkat cell lysate

Predicted band size: 49 kDa

Observed band size: 49 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1E1-E8-H4

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human, African green monkey

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TUBB. The exact immunogen used to generate this antibody is proprietary information.

P07437

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/300", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification technique

Cross-flow filtration

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB

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Publications (30)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40806783

2025

CFAP300 Loss-of-Function Mutations with Primary Ciliary Dyskinesia: Evidence from Ex Vivo and ALI Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Anna G Demchenko,Tatiana A Kyian,Elena I Kondratyeva,Elizaveta E Bragina,Oksana P Ryzhkova,Roman V Veiko,Aleksandra G Nazarova,Vyacheslav B Chernykh,Svetlana A Smirnikhina,Sergey I Kutsev

Cells 14: PubMed40498007

2025

Modulation of ER Stress and Inflammation by S-Ketamine, R-Ketamine, and Their Metabolites in Human Microglial Cells: Insights into Novel Targets for Depression Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Jóźwiak-Bębenista,Anna Wiktorowska-Owczarek,Małgorzata Siatkowska,Piotr Komorowski,Aneta Włodarczyk,Edward Kowalczyk,Paulina Sokołowska

Andrology 13:1848-1865 PubMed39749764

2025

Role of GPR55 receptor in bovine sperm capacitation.

Applications

Unspecified application

Species

Unspecified reactive species

Raquel Lottero-Leconte,Angela Lara,Jessica Plaza,Camila Arroyo-Salvo,María Eugenia Bogetti,Amada Eugenia Ynsaurralde Rivolta,Franco Dellavalle,Fiamma Sengiali,Pablo Cetica,Sofía Rio,Lucia Zalazar,Andreína Cesari,Marcelo Miragaya,Sergio Morado,Silvina Perez-Martinez

Andrology 13:382-395 PubMed38804843

2024

Effect of bicarbonate and polyvinyl alcohol on in vitro capacitation and fertilization ability of cryopreserved equine spermatozoa.

Applications

Unspecified application

Species

Unspecified reactive species

Camila Arroyo-Salvo,Sofía Río,María Eugenia Bogetti,Jessica Plaza,Marcelo Miragaya,Agustín Yaneff,Carlos Davio,Rafael Fissore,María Gracia Gervasi,Andrés Gambini,Silvina Perez-Martinez

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38692734

2024

Mutation of the ALS-/FTD-Associated RNA-Binding Protein FUS Affects Axonal Development.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca W van Tartwijk,Lucia C S Wunderlich,Ioanna Mela,Stanislaw Makarchuk,Maximilian A H Jakobs,Seema Qamar,Kristian Franze,Gabriele S Kaminski Schierle,Peter H St George-Hyslop,Julie Qiaojin Lin,Christine E Holt,Clemens F Kaminski

The EMBO journal 43:168-195 PubMed38212382

2024

Brown adipose tissue CoQ deficiency activates the integrated stress response and FGF21-dependent mitohormesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Fang Chang,Amanda L Gunawan,Irene Liparulo,Peter-James H Zushin,Kaitlyn Vitangcol,Greg A Timblin,Kaoru Saijo,Biao Wang,Güneş Parlakgül,Ana Paula Arruda,Andreas Stahl

Synapse (New York, N.Y.) 77:e22266 PubMed36811190

2023

Upregulation of SLITRK5 in patients with epilepsy and in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Linming Zhang,Mingda Ai,Di Xia,Hongyu Chen,Ruijing Pang,Rong Mei,Lianmei Zhong,Ling Chen

International journal of molecular sciences 24: PubMed36835655

2023

YTHDF1 Attenuates TBI-Induced Brain-Gut Axis Dysfunction in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Peizan Huang,Min Liu,Jing Zhang,Xiang Zhong,Chunlong Zhong

International journal of molecular sciences 24: PubMed36614135

2022

Intranasal Administration of Forskolin and Noopept Reverses Parkinsonian Pathology in PINK1 Knockout Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ruben K Dagda,Raul Y Dagda,Emmanuel Vazquez-Mayorga,Bridget Martinez,Aine Gallahue

Antioxidants (Basel, Switzerland) 12: PubMed36670876

2022

CoQ Regulates Brown Adipose Tissue Respiration and Uncoupling Protein 1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Fang Chang,Amanda L Gunawan,Irene Liparulo,Peter-James H Zushin,Ambre M Bertholet,Yuriy Kirichok,Andreas Stahl

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (AB131205)

Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (AB131205)

Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (AB131205)

Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (AB131205)

Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Loading Control (AB131205)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (30)

Product promise