Anti-beta Tubulin antibody [AA2] - Loading Control

3 product images

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  Western blot - Anti-beta Tubulin antibody [AA2] - Loading Control (ab231082)

  Beta Tubulin Western blot staining using mouse Anti-beta Tubulin antibody

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab231082 and Anti-GAPDH antibody - Loading Control ab9485 (Rabbit anti-GAPDH loading control) overnight at 4°C at 1ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  All lanes: Western blot - Anti-beta Tubulin antibody [AA2] - Loading Control (ab231082)

  Lane 1: HeLa whole cell lysate at 20 µg

  Lane 2: NIH3T3 whole cell lysate at 20 µg

  Lane 3: PC12 whole cell lysate at 20 µg

  Performed under non-reducing conditions.

  Predicted band size: 49 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [AA2] (ab231082)

  ab231082 staining beta Tubulin in A431 (human epidermoid carcinoma cell line) cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab231082 at 1μg/ml. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
  

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [AA2] (ab231082)

  IHC image of beta tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon carcinoma* performed on a Leica BOND^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab231082, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre