Anti-beta-Tubulin antibody - BSA free [BLR236K] (ab314069)

Rabbit Recombinant Monoclonal Beta-5-tubulin antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TUBB aa 1-50.

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Images

Flow Cytometry - Anti-beta-Tubulin antibody [BLR236K] - BSA free (AB314069), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human TUBB aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P07437

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt	ICC	IHC-P	IP	WB
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Target data

See full target information TUBB

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Alternative names

TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human TUBB aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P07437
Clone number: BLR236K
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: Up to 12 months
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta V tubulin also known as TUBB6 is a microtubule protein involved in the mechanical structure of cells. It plays a role in the formation of microtubules by polymerizing with alpha tubulin allowing tubulin dimers to stack into spirals that form hollow tubes. This protein has a molecular mass of approximately 49 kDa. Beta V tubulin is expressed in many tissues with higher levels observed in the human brain liver and testis.

Biological function summary

Beta V tubulin is important for maintaining cellular architecture and intracellular transport. It is an important component of the cytoskeleton a complex that provides support and shape to the cell and facilitates movement of organelles within the cell. Microtubules formed by beta V tubulin are essential for mitosis as they organize and separate chromosomes during cell division. This role connects beta V tubulin to cell cycle regulation and stability.

Pathways

Beta V tubulin participates in the microtubule organizing center pathways and impacts the cell cycle pathway. It interacts with proteins including kinesin and dynein which are motor proteins that move along microtubules to transport cellular cargo. These processes ensure proper cellular function by delivering necessary molecules and considering structural integrity linking beta V tubulin to essential cellular processes.

Associated diseases and disorders

Dysregulation of beta V tubulin has been linked to certain types of cancer particularly liver and breast cancer. It associates with p53 a protein critical for tumor suppression indicating a potential role in tumorigenesis. Additionally mutations in beta V tubulin can contribute to neurodevelopmental disorders due to its expression in the brain and its involvement in neuronal function. This association highlights the importance of beta V tubulin in both normal cellular functioning and disease development.

Product promise

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9 product images

Flow Cytometry - Anti-beta-Tubulin antibody [BLR236K] - BSA free (ab314069)
Detection of human beta-Tubulin (shaded) in K562 cells by flow cytometry. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody [BLR236K] (A700-236CF) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Flow Cytometry - Anti-beta-Tubulin antibody [BLR236K] - BSA free (ab314069)
Detection of mouse beta-Tubulin (shaded) in A20 cells by flow cytometry. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody [BLR236K] (A700-236CF) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Western blot - Anti-beta-Tubulin antibody [BLR236K] - BSA free (ab314069)
Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody [BLR236K] (A700-236 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].
All lanes: Western blot - Anti-beta-Tubulin antibody [BLR236K] - BSA free (ab314069) at 1/1000 dilution
Lane 1: NIH 3T3 Whole cell lysate at 10 µg
Lane 2: CT26 Whole cell lysate at 10 µg
Lane 3: CH27 Whole cell lysate at 10 µg
Lane 4: TCMK-1 Whole cell lysate at 10 µg
Lane 5: BW5147.3 Whole cell lysate at 10 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Western blot - Anti-beta-Tubulin antibody [BLR236K] - BSA free (ab314069)
Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Hep-G2, Jurkat, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody [BLR236K] (A700-236CF) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].
All lanes: Western blot - Anti-beta-Tubulin antibody [BLR236K] - BSA free (ab314069) at 1/1000 dilution
Lane 1: HeLa Whole cell lysate at 10 µg
Lane 2: HEK293T Whole cell lysate at 10 µg
Lane 3: Hep-G2 Whole cell lysate at 10 µg
Lane 4: Jurkat Whole cell lysate at 10 µg
Lane 5: MOLT-4 Whole cell lysate at 10 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Immunoprecipitation - Anti-beta-Tubulin antibody [BLR236K] - BSA free (ab314069)
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-beta-Tubulin recombinant monoclonal antibody [BLR236K] (A700-236-1) used for IP at 20 µl/mg lysate. beta-Tubulin was also immunoprecipitated by a second antibody against a different epitope of beta-Tubulin (BL-2134A-6D11). For blotting immunoprecipitated beta-Tubulin, A700-236 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta-Tubulin antibody [BLR236K] - BSA free (ab314069)
Detection of mouse beta-Tubulin by immunohistochemistry. Sample: FFPE section of mouse testis. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody [BLR236K] (A700-236CF). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta-Tubulin antibody [BLR236K] - BSA free (ab314069)
Detection of human beta-Tubulin by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody [BLR236K] (A700-236CF). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry - Anti-beta-Tubulin antibody [BLR236K] - BSA free (ab314069)
Detection of human beta-Tubulin by immunocytochemistry. Sample: FFPE section of MOLT-4 cells. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody [BLR236K] (A700-236CF). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry - Anti-beta-Tubulin antibody [BLR236K] - BSA free (ab314069)
Detection of mouse beta-Tubulin by immunocytochemistry. Sample: FFPE section of ND7/23 cells. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody [BLR236K] (A700-236CF). Secondary: HRP-conjugated goat anti-rabbit IgG.