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Rabbit Recombinant Monoclonal Beta-5-tubulin antibody. Microtubule marker. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 42 publications.


Images

Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (AB52901), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (AB52901), expandable thumbnail
  • Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (AB52901), expandable thumbnail
  • Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (AB52901), expandable thumbnail
  • Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (AB52901), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested
Zebrafish
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Zebrafish
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/20000
Notes

-

Species
Rat
Dilution info
1/20000
Notes

-

Species
Human
Dilution info
1/20000
Notes

-

Predicted
Predicted

Species
Zebrafish
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Alternative names

TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain

Recommended products

Rabbit Recombinant Monoclonal Beta-5-tubulin antibody. Microtubule marker. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 42 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1331Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Associated diseases and disorders

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901), expandable thumbnail

    Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52901 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    All lanes: Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/20000 dilution

    Lane 1: Brain (Mouse) Tissue Lysate at 20 µg

    Lane 2: Brain (Rat) Tissue Lysate at 20 µg

    Lane 3: Spinal Cord (Mouse) Tissue Lysate at 20 µg

    Lane 4: Spinal Cord (Rat) Tissue Lysate at 20 µg

    Lane 5: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) ab175781) at 1/10000 dilution

    Predicted band size: 49 kDa

    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)

    ab52901 at 1/250 dilution staining beta Tubulin in human gastric carcinoma by Immunohistochemsitry, Paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901), expandable thumbnail

    Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)

    All lanes: Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/20000 dilution

    All lanes: HeLa cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP-conjugated at 1/2000 dilution

    Predicted band size: 49 kDa

    Observed band size: 50 kDa

  • Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)

    Blocking Step: 5% Milk for 1 hour at 23°C

    All lanes: Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/1000 dilution

    All lanes: 293 human embryonic kidney whole cell lysate at 25 µg

    Secondary

    All lanes: An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Observed band size: 55 kDa

    Exposure time: 45s

  • Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901), expandable thumbnail

    Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)

    In Western blot, Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-Flagellin antibody [EPR28356-51] (Anti-Flagellin antibody [EPR28356-51] ab316010) at 1/1000 dilution

    Lane 1: E. coli BL21(DE3) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 51 kDa, 50 kDa

    Exposure time: 180s

  • Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901), expandable thumbnail

    Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: human cerebellum.
    In Western blot, anti-Anti-beta Tubulin antibody (ab52901) staining at 1/20000 dilution.

    All lanes: Western blot - Anti-Mitochondrial Ferritin antibody [EPR1797] (Anti-Mitochondrial Ferritin antibody [EPR1797] ab124889) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Human cerebellum tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 28 kDa

    Observed band size: 21 kDa

    Exposure time: 180s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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