Anti-beta Tubulin antibody [EPR16774]

19 product images

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  Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Blocking/Dilution buffer: 5% NFDM/TBST.

  The immunogen of ab179513 has 67% identities with beta I Tubulin. The WB image shows it cross-reacts with beta I Tubulin.
  Human beta I Tubulin is an in house recombinant protein (aa1-451) with a proprietary tag.

  All lanes: Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/1000 dilution

  All lanes: Human beta I Tubulin recombinant protein at 0.01 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

  Predicted band size: 49 kDa

  Observed band size: 50 kDa

• 

  Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta Tubulin with ab179513 at 1/1000 dilution, followed by anti-rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and anti-mouse AlexaFluor® 594 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/500 dilution (red).
  The negative controls are as follows;
  -ve control 1: ab179513 at 1/1000 dilution followed by anti-mouse AlexaFluor® 594 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/500 dilution.
  -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by anti-rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/500 dilution.

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  Flow Cytometry (Intracellular) - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta Tubulin with ab179513 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
  

• 

  Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Blocking/Dilution buffer: 5% NFDM/TBST.

  The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

  All lanes: Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

  Lane 1: Zebrafish whole lysates at 20 µg

  Lane 2: Xenopus tropicalis lysates at 20 µg

  Lane 3: Drosophila whole lysates at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

  Predicted band size: 49 kDa

  Observed band size: 50 kDa

• 

  Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Blocking/Dilution buffer: 5% NFDM/TBST.

  The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

  All lanes: Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

  Lane 1: UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates at 20 µg

  Lane 2: MDCK (Canine kidney cell line) whole cell lysates at 20 µg

  Lane 3: MDBK (Bovine kidney cell line) whole cell lysates at 20 µg

  Lane 4: COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

  Predicted band size: 49 kDa

  Observed band size: 50 kDa

• 

  Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Blocking/Dilution buffer: 5% NFDM/TBST.

  The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

  All lanes: Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

  Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

  Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg

  Lane 3: A431 (Human epidermoid carcinoma) whole cell lysates at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

  Predicted band size: 49 kDa

  Observed band size: 50 kDa

• 

  Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Blocking/Dilution buffer: 5% NFDM/TBST.

  The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

  All lanes: Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

  Lane 1: Human fetal brain lysates at 10 µg

  Lane 2: Human fetal kidney lysates at 10 µg

  Secondary

  All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

  Predicted band size: 49 kDa

  Observed band size: 50 kDa

• 

  Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Blocking/Dilution buffer: 5% NFDM/TBST.

  The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

  All lanes: Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

  Lane 1: Mouse brain lysates at 10 µg

  Lane 2: Rat brain lysates at 10 µg

  Lane 3: C6 (Rat glial tumor cells) whole cell lysates at 10 µg

  Lane 4: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg

  Lane 5: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg

  Lane 6: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

  Predicted band size: 49 kDa

  Observed band size: 50 kDa

• 

  Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling Mouse IgG1 with Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control ab280974 at 1/20 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
  

  Confocal image showing no staining in RAW 264.7 cells.

  Negative control 1: Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control ab280974 at a 1/20 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at a 1/1000 dilution.

  Negative control 2: ab179513 at a 1/200 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 at a 1/1000 dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on neurons of human cerebral cortex is observed. Counter stained with Hematoxylin.
  

  Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on tubules and the glomerulus of human kidney is observed. Counter stained with Hematoxylin.
  

  Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on human glioma is observed. Counter stained with Hematoxylin.
  

  Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on neurons of mouse cerebral cortex is observed. Counter stained with Hematoxylin.
  

  Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on neurons of rat cerebral cortex is observed. Counter stained with Hematoxylin.
  

  Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  MCF7/ HCT 116 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] ab70475 at 1:100 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with Anti-Integrin beta 3 antibody [Y2/51] ab9509 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
  
  Confocal image showing membranous and cytoplasmic staining in MCF7 cells.
  Negative control: HCT 116 (PMID: 14998492)

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  Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  NIH/3T3 cells were fixed in 100% methanol and permeabilized with 0.1% Triton X-100. Primary antibody, Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade ab52484 at 1:2000 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade ab52484 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  RAW 264.7 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, Anti-CD68 antibody [FA-11] ab53444 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with Anti-CD68 antibody [FA-11] ab53444 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI. Confocal image showing cytoplasmic staining in RAW 264.7 cell line.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Confocal image showing membranous staining in RAW 264.7 cells.
  
  RAW 264.7, WEHI-231 and THP-1 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, Anti-CD11b antibody [M1/70] ab8878 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488- conjugated Goat anti-Rat secondary antibody (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with Anti-CD11b antibody [M1/70] ab8878 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
  
  Negative control: WEHI-231 (PMID: 2457584)

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  Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] (ab179513)

  Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte) cells labeling SHP2 with Anti-SHP2 antibody [79/PTP1D/SHP2] ab290646 at 1/50 (20.18 µg/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in Jurkat cell line is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10 µg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
  
  Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 µg/ml dilution.