Anti-beta Tubulin antibody [EPR16774] - Loading Control

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labeling alpha Tubulin (acetyl K40) with ab289863 at 1/100 dilution (10.81 µg/ml), followed by ab96971 Goat Anti-Rat IgG Fc (DyLight^® 488) preadsorbed antibody at 1/500 dilution (Green).

Confocal image showing increased cytoplasmic staining in HeLa cells treated with Trichostatin A (500 ng/ml) for 4 hours.

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red).

The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : PBS was used instead of primary antibody followed by secondary antibody is ab96971 Goat Anti-Rat IgG Fc (DyLight^® 488) preadsorbed at 1/500 dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta Tubulin with ab179513 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta Tubulin with ab179513 at 1/1000 dilution, followed by anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1 : ab179513 at 1/1000 dilution followed by anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by anti-rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized transfected 293T (human embryonic kidney epithelial cell) cells labelling Apolipoprotein E4 with ab169861 at 1/50 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution(Green).

Confocal image showing cytoplamic staining in HEK-293T cells transfected with a human APOE4(C112R) expression vector containing a myc tag. (shown in green). The counterstain was observed in red. Nuclear DNA was labelled with DAPI (shown in blue).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counter stain tubulin at a 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/500 dilution (Red).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on human glioma is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on neurons of human cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on tubules and the glomerulus of human kidney is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized SH-SY5Y cells with ab86808 at 1/50 dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used for counterstain at a 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/500 dilution (Red). Nuclear DNA was labelled with DAPI (shown in blue)

Confocal image showing cytoplasmic and weak nuclear staining in SH-SY5Y cells and no staining in LNCaP cells.
Negative control : LNCaP(PMID : 17929277)

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells with ab10543 at 1/8000 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used as a counterstain at a 1/200 dilution followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at a 1/1000 dilution (Magenta).

Confocal image showing nuclear staining in HeLa cells (shown in green) at metaphase, the signal is decreased after λ Protein phosphatase treatment at 30℃ for 2 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte) cells labeling SHP2 with ab290646 at 1/50 (20.18 µg/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in Jurkat cell line is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10 µg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 µg/ml dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

MCF7/ HCT 116 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab70475 at 1 : 100 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab9509 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI. Confocal image showing membranous and cytoplasmic staining in MCF7 cells. Negative control : HCT 116 (PMID : 14998492)

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Hsp27 with ab2790 at 1/100 dilution, followed by AlexaFluor® 488-conjugated Goat anti-Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab9509 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling EGFR with ab231 at 1/50 dilution followed by secondary antibody ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (green).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used as a counterstain at a 1/200 dilution followed by a secondary antibody ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) used at a 1/1000 dilution (red).

Confocal image showing strong membranous and weak cytoplasmic staining in A431 cells.
Low expression control : HEK-293 (PMID : 26368334).

Nuclear DNA was labelled with DAPI (shown in blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling CEACAM1 + CEACAM3 + CEACAM6 with ab104450 at 1/250 dilution followed by secondary antibody ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (green).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used as a counterstain at a 1/200 dilution followed by a secondary antibody ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) used at a 1/1000 dilution (red).

Confocal image showing membranous staining in HepG2 cell line.
Negative control : HeLa (PMID : 11580753).

Nuclear DNA was labelled with DAPI (shown in blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A375 (human malignant melanoma epithelial cell) cells labelling SOX10 with ab218522 at 1/50 dilution followed by secondary antibody ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (green).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used as a counterstain at a 1/200 dilution followed by a secondary antibody ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/1000 dilution (red).

Confocal image showing nuclear staining in A375 cells.
Negative control : MCF7 (PMID : 23799842).
Nuclear DNA was labelled with DAPI (shown in blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling AICDA with ab323835 at 1/100 (10 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in Raji cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : K-562.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 (10 ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab323835 at a 1/100 dilution followed by ab150088 at a 1/1000 dilution.

-ve control 2 : ab179513 at a 1/200 dilution followed by ab150117 at a 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 cells labeling alpha Tubulin (acetyl K40) with ab289863 at 1/100 dilution (10.81 µg/ml), followed by ab96971 Goat Anti-Rat IgG Fc (DyLight^® 488) preadsorbed antibody at 1/500 dilution (Green).

Confocal image showing increased cytoplasmic staining in C6 cells treated with Trichostatin A (500 ng/ml) for 4 hours. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red).

The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : PBS was used instead of primary antibody followed by secondary antibody is ab96971 Goat Anti-Rat IgG Fc (DyLight^® 488) preadsorbed at 1/500 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

RAW 264.7 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab53444 at 1 : 50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab53444 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI. Confocal image showing cytoplasmic staining in RAW 264.7 cell line.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

NIH/3T3 cells were fixed in 100% methanol and permeabilized with 0.1% Triton X-100. Primary antibody, ab52484 at 1 : 2000 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-mouse secondary antibody (ab150117) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab52484 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of rat cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Confocal image showing membranous staining in RAW 264.7 cells. RAW 264.7, WEHI-231 and THP-1 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab8878 at 1 : 50 was incubated overnight at 4° C, followed by AlexaFluor® 488- conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab8878 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI. Negative control : WEHI-231 (PMID : 2457584)

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized Neuro-2a cells with ab86808 at 1/50 dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used for counterstain at a 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/500 dilution (Red). Nuclear DNA was labelled with DAPI (shown in blue)

Confocal image showing mainly cytoplasmic staining in Neuro-2a cells.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized PC-12 cells with ab86808 at 1/50 dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used for counterstain at a 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/500 dilution (Red). Nuclear DNA was labelled with DAPI (shown in blue)

Confocal image showing mainly cytoplasmic staining in PC-12 cells

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling Mouse IgG1 with ab280974 at 1/20 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

Confocal image showing no staining in RAW 264.7 cells.

Negative control 1 : ab280974 at a 1/20 dilution followed by ab150080 at a 1/1000 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labeling alpha Tubulin (acetyl K40) with ab289863 at 1/100 dilution (10.81 µg/ml), followed by ab96971 Goat Anti-Rat IgG Fc (DyLight^® 488) preadsorbed antibody at 1/500 dilution (Green).

Confocal image showing increased cytoplasmic staining in NIH/3T3 cells treated with Trichostatin A (500 ng/ml) for 4 hours. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red).

The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : PBS was used instead of primary antibody followed by secondary antibody is ab96971 Goat Anti-Rat IgG Fc (DyLight^® 488) preadsorbed at 1/500 dilution.

• WB

Supplier Data

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Blocking/Dilution buffer : 5% NFDM/TBST.

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

All lanes:

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (ab179513) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg

Lane 3:

A431 (Human epidermoid carcinoma) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

• WB

Lab

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

This blot was produced using 4-20% SDS-PAGE containing 15 μg of Jurkat whole cell lysate per lane at 150V for 1hr before being transferred onto a 0.45 μm PVDF membrane at 75V for 1hr. The membrane was then blocked for 1hr using 5% NFDM/TBST, then incubated with ab179513 (1/1000) at room temperature for 1hr. After being washed three times in TBST, the membrane was incubated with Peroxidase conjugated goat anti-rabbit IgG (H+L) (ab97051) at 1/20,000 dilution for 1hr at room temperature. The membrane was washed three times again. Then the signal was developed using the ECL technique. ab179513 was stored at a range of temperatures (+4°C, +22°C, +37°C) for 1 week before being tested in WB. The image shows the band intensity remains relatively constant across all storage temperatures, demonstrating that antibody activity is not affected.

All lanes:

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (ab179513) at 1/1000 dilution

All lanes:

Jurkat whole cell lysate at 15 µg with NDFM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Blocking/Dilution buffer : 5% NFDM/TBST.

The immunogen of ab179513 has 67% identities with beta I Tubulin. The WB image shows it cross-reacts with beta I Tubulin.
Human beta I Tubulin is an in house recombinant protein (aa1-451) with a proprietary tag.

All lanes:

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (ab179513) at 1/1000 dilution

All lanes:

Human beta I Tubulin recombinant protein at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Blocking/Dilution buffer : 5% NFDM/TBST.

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

All lanes:

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (ab179513) at 1/2000 dilution

Lane 1:

Human fetal brain lysates at 10 µg

Lane 2:

Human fetal kidney lysates at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Blocking/Dilution buffer : 5% NFDM/TBST.

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

All lanes:

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (ab179513) at 1/2000 dilution

Lane 1:

Mouse brain lysates at 10 µg

Lane 2:

Rat brain lysates at 10 µg

Lane 3:

C6 (Rat glial tumor cells) whole cell lysates at 10 µg

Lane 4:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg

Lane 6:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Blocking/Dilution buffer : 5% NFDM/TBST.

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

All lanes:

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (ab179513) at 1/2000 dilution

Lane 1:

UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates at 20 µg

Lane 2:

MDCK (Canine kidney cell line) whole cell lysates at 20 µg

Lane 3:

MDBK (Bovine kidney cell line) whole cell lysates at 20 µg

Lane 4:

COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

Blocking/Dilution buffer : 5% NFDM/TBST.

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.

All lanes:

Western blot - Anti-beta Tubulin antibody [EPR16774] - Loading Control (ab179513) at 1/2000 dilution

Lane 1:

Zebrafish whole lysates at 20 µg

Lane 2:

Xenopus tropicalis lysates at 20 µg

Lane 3:

Drosophila whole lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false