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Anti-beta Tubulin antibody (ab15568) is a rabbit polyclonal antibody that is used to detect beta Tubulin in Western Blot, IHC-P, ICC/IF. Suitable for Human samples.



- Over 100 publications

- Trusted since 2004


Images

Western blot - Anti-beta Tubulin antibody - Loading Control (AB15568), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (AB15568), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (AB15568), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody - Loading Control (AB15568), expandable thumbnail
  • Western blot - Anti-beta Tubulin antibody - Loading Control (AB15568), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Chimpanzee
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Fungi
Predicted
Predicted
Predicted
Guinea pig
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Rice
Predicted
Predicted
Predicted
Saccharomyces cerevisiae
Predicted
Predicted
Predicted
Sea urchin
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted
Xenopus tropicalis
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/200
Notes

Antigen retrieval is not essential but may optimise staining.

Predicted
Predicted

Species
Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Saccharomyces cerevisiae, Xenopus laevis, Chimpanzee, Sea urchin, Fungi, Rice, Xenopus tropicalis, Dog
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Saccharomyces cerevisiae, Xenopus laevis, Chimpanzee, Sea urchin, Fungi, Rice, Xenopus tropicalis, Dog
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Saccharomyces cerevisiae, Xenopus laevis, Chimpanzee, Sea urchin, Fungi, Rice, Xenopus tropicalis, Dog
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Alternative names

Recommended products

Anti-beta Tubulin antibody (ab15568) is a rabbit polyclonal antibody that is used to detect beta Tubulin in Western Blot, IHC-P, ICC/IF. Suitable for Human samples.



- Over 100 publications

- Trusted since 2004

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique
Affinity purification Immunogen
Specificity

Homology with Tubulin beta chain and Tubulin beta-3 chain

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-beta Tubulin antibody (ab15568) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of beta Tubulin?


Anti-beta Tubulin (ab15568) specifically detects a band for beta Tubulin (UniProt: Q13509) at a molecular weight of 55kDa.

Trusted by the scientific community


Anti-beta Tubulin (ab15568) was first used in a scientific publication in 2004 and has been cited over 100 times in peer-reviewed journals.

Reviewed by scientists


Anti-beta Tubulin (ab15568) has over 5 independent reviews from customers.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beta tubulin also known as beta-tubulin or Β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Associated diseases and disorders

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

Product promise

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6 product images

  • Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568), expandable thumbnail

    Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568)

    Beta Tubulin Western blot staining using rabbit Anti-beta Tubulin antibody

    All lanes: Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568) at 1 µg/mL

    Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg

    Lane 2: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 49 kDa

    Observed band size: 55 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (ab15568), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (ab15568)

    Beta Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-beta Tubulin antibody

    ICC/IF image of ab15568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (ab15568), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (ab15568)

    Beta Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-beta Tubulin antibody

    ab15568 staining beta Tubulin in IMR32 Human neuroblastoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA containing TBST) for 16 hours at 4°C. A Cy3®-conjugated Goat anti-rabbit IgG polyclonal (1:1000) was used as the secondary antibody. Blue - nuclei (DAPI).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody - Loading Control (ab15568), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody - Loading Control (ab15568)

    Beta Tubulin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-beta Tubulin antibody

    ab15568 staining beta Tubulin in Human lung by Immunohistochemistry (FFPE-sections).

  • Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568), expandable thumbnail

    Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568)

    Beta Tubulin Western blot staining using rabbit Anti-beta Tubulin antibody

    All lanes: Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568) at 1 µg/mL

    All lanes: Western blot - Recombinant Human beta Tubulin protein (Recombinant Human beta Tubulin protein ab70187) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Exposure time: 30s

  • Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568), expandable thumbnail

    Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568)

    Beta Tubulin Western blot staining using rabbit Anti-beta Tubulin antibody

    All lanes: Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568) at 1 µg/mL

    All lanes: Western blot - Recombinant Human beta Tubulin protein (Recombinant Human beta Tubulin protein ab70187) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Exposure time: 30s

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Product protocols

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