Anti-beta Tubulin antibody (ab15568) is a rabbit polyclonal antibody detecting beta Tubulin in Western Blot, IHC-P, ICC/IF. Suitable for Human.
- Over 100 publications
- Trusted since 2004
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- Polymers 15:2023Evaluation of a Peptide Hydrogel as a Chondro-Instructive Three-Dimensional Microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRodrigo Nogoceke,Raphaella Josino,Anny Waloski Robert,Marco Augusto StimamiglioPubMed 38139882
- Communications biology 6:11272023Synaptic proteasome is inhibited in Alzheimer's disease models and associates with memory impairment in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFelipe C Ribeiro,Danielle Cozachenco,Luana Heimfarth,Juliana T S Fortuna,Guilherme B de Freitas,Jorge M de Sousa,Soniza V Alves-Leon,Renata E P Leite,Claudia K Suemoto,Lea T Grinberg,Fernanda G De Felice,Mychael V Lourenco,Sergio T FerreiraPubMed 37935829
- Biomaterials research 27:1032023Time-sequential fibroblast-to-myofibroblast transition in elastin-variable 3D hydrogel environments by collagen networks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNhuan T Do,Sun Young Lee,Yoon Seo Lee,ChaeHo Shin,Daeho Kim,Tae Geol Lee,Jin Gyeong Son,Se-Hwa KimPubMed 37848974
- Frontiers in cell and developmental biology 11:11106812023Juno and CD9 protein network organization in oolemma of mouse oocyte.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichaela Frolikova,Vishma Pratap Sur,Ivan Novotny,Michaela Blazikova,Jana Vondrakova,Ondrej Simonik,Lukas Ded,Eliska Valaskova,Lenka Koptasikova,Ales Benda,Pavla Postlerova,Ondrej Horvath,Katerina KomrskovaPubMed 37635875
- Cancers 15:2023Single Fraction and Hypofractionated Radiation Cause Cochlear Damage, Hearing Loss, and Reduced Viability of Merlin-Deficient Schwann Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristine T Dinh,Si Chen,Aida Nourbakhsh,Kyle Padgett,Perry Johnson,Stefania Goncalves,Olena Bracho,Esperanza Bas,Jorge Bohorquez,Paula V Monje,Cristina Fernandez-Valle,Nagy Elsayyad,Xuezhong Liu,Scott M Welford,Fred TelischiPubMed 37345155
- International journal of molecular sciences 24:2023Approaches in Hydroxytyrosol Supplementation on Epithelial-Mesenchymal Transition in TGFβ1-Induced Human Respiratory Epithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRabiatul Adawiyah Razali,Muhammad Dain Yazid,Aminuddin Saim,Ruszymah Bt Hj Idrus,Yogeswaran LokanathanPubMed 36835384
- Frontiers in pharmacology 14:10906542023CTH/MPST double ablation results in enhanced vasorelaxation and reduced blood pressure upregulation of the eNOS/sGC pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAntonia Katsouda,Maria Markou,Paraskevas Zampas,Aimilia Varela,Constantinos H Davos,Valentina Vellecco,Giuseppe Cirino,Mariarosaria Bucci,Andreas PapapetropoulosPubMed 36860295
- Diseases of aquatic organisms 153:17-292023Mass mortality of collector urchins Tripneustes gratilla in Hawai`i.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThierry M Work,Julie Dagenais,Bob Rameyer,Renee Breeden,Tina M WeatherbyPubMed 36727688
- IBRO neuroscience reports 14:235-2432023Neural fate commitment of rat full-term amniotic fluid stem cells three-dimensional embryoid bodies and neurospheres formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNuratiqah Azmi,Siti Sarah Mustaffa Al Bakri,Winnie Khor,Siti Nurusaadah Hamzah,Nurfarhana Ferdaos,King-Hwa Ling,Norshariza NordinPubMed 37388489
- Nature 613:160-1682022CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin Qian,Marcus J Tol,Jin Wu,Lauren F Uchiyama,Xu Xiao,Liujuan Cui,Alexander H Bedard,Thomas A Weston,Pradeep S Rajendran,Laurent Vergnes,Yuta Shimanaka,Yesheng Yin,Yasaman Jami-Alahmadi,Whitaker Cohn,Bryce T Bajar,Chia-Ho Lin,Benita Jin,Laura A DeNardo,Douglas L Black,Julian P Whitelegge,James A Wohlschlegel,Karen Reue,Kalyanam Shivkumar,Feng-Jung Chen,Stephen G Young,Peng Li,Peter TontonozPubMed 36477540
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Fungi | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rice | Predicted | Predicted | Predicted |
| Saccharomyces cerevisiae | Predicted | Predicted | Predicted |
| Sea urchin | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes Antigen retrieval is not essential but may optimise staining. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Saccharomyces cerevisiae, Xenopus laevis, Chimpanzee, Sea urchin, Fungi, Rice, Xenopus tropicalis, Dog | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Saccharomyces cerevisiae, Xenopus laevis, Chimpanzee, Sea urchin, Fungi, Rice, Xenopus tropicalis, Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Saccharomyces cerevisiae, Xenopus laevis, Chimpanzee, Sea urchin, Fungi, Rice, Xenopus tropicalis, Dog | Dilution info - | Notes - |
Associated Products
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Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Alternative names
TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain
Recommended products
Anti-beta Tubulin antibody (ab15568) is a rabbit polyclonal antibody detecting beta Tubulin in Western Blot, IHC-P, ICC/IF. Suitable for Human.
- Over 100 publications
- Trusted since 2004
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Homology with Tubulin beta chain and Tubulin beta-3 chain
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-beta Tubulin antibody (ab15568) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of beta Tubulin?
Anti-beta Tubulin (ab15568) specifically detects a band for beta Tubulin (UniProt: Q13509) at a molecular weight of 55kDa.
Trusted by the scientific community
Anti-beta Tubulin (ab15568) was first used in a scientific publication in 2004 and has been cited over 100 times in peer-reviewed journals.
Reviewed by scientists
Anti-beta Tubulin (ab15568) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta tubulin also known as beta-tubulin or Β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.
- Biological function summary
This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.
- Pathways
Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.
- Associated diseases and disorders
Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568)
Beta Tubulin Western blot staining using rabbit Anti-beta Tubulin antibody
All lanes: Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568) at 1 µg/mL
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 49 kDa
Observed band size: 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (ab15568)
Beta Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-beta Tubulin antibody
ICC/IF image of ab15568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (ab15568)
Beta Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-beta Tubulin antibody
ab15568 staining beta Tubulin in IMR32 Human neuroblastoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA containing TBST) for 16 hours at 4°C. A Cy3®-conjugated Goat anti-rabbit IgG polyclonal (1:1000) was used as the secondary antibody. Blue - nuclei (DAPI).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody - Loading Control (ab15568)
Beta Tubulin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-beta Tubulin antibody
ab15568 staining beta Tubulin in Human lung by Immunohistochemistry (FFPE-sections).
Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568)
Beta Tubulin Western blot staining using rabbit Anti-beta Tubulin antibody
All lanes: Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568) at 1 µg/mL
All lanes: Western blot - Recombinant Human beta Tubulin protein (Recombinant Human beta Tubulin protein ab70187) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Exposure time: 30s
Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568)
Beta Tubulin Western blot staining using rabbit Anti-beta Tubulin antibody
All lanes: Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568) at 1 µg/mL
All lanes: Western blot - Recombinant Human beta Tubulin protein (Recombinant Human beta Tubulin protein ab70187) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Exposure time: 30s
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