Anti-beta Tubulin antibody (ab15568) is a rabbit polyclonal antibody detecting beta Tubulin in Western Blot, IHC-P, ICC/IF. Suitable for Human.
- Over 100 publications
- Trusted since 2004
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted |
Chicken | Predicted | Predicted | Predicted |
Chimpanzee | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted |
Dog | Predicted | Predicted | Predicted |
Fungi | Predicted | Predicted | Predicted |
Guinea pig | Predicted | Predicted | Predicted |
Pig | Predicted | Predicted | Predicted |
Rice | Predicted | Predicted | Predicted |
Saccharomyces cerevisiae | Predicted | Predicted | Predicted |
Sea urchin | Predicted | Predicted | Predicted |
Xenopus laevis | Predicted | Predicted | Predicted |
Xenopus tropicalis | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200 | Notes Antigen retrieval is not essential but may optimise staining. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Saccharomyces cerevisiae, Xenopus laevis, Chimpanzee, Sea urchin, Fungi, Rice, Xenopus tropicalis, Dog | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Saccharomyces cerevisiae, Xenopus laevis, Chimpanzee, Sea urchin, Fungi, Rice, Xenopus tropicalis, Dog | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Saccharomyces cerevisiae, Xenopus laevis, Chimpanzee, Sea urchin, Fungi, Rice, Xenopus tropicalis, Dog | Dilution info - | Notes - |
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Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain
Anti-beta Tubulin antibody (ab15568) is a rabbit polyclonal antibody detecting beta Tubulin in Western Blot, IHC-P, ICC/IF. Suitable for Human.
- Over 100 publications
- Trusted since 2004
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Homology with Tubulin beta chain and Tubulin beta-3 chain
Beta tubulin also known as beta-tubulin or Β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.
This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.
Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.
Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Beta Tubulin Western blot staining using rabbit Anti-beta Tubulin antibody
All lanes: Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568) at 1 µg/mL
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 49 kDa
Observed band size: 55 kDa
Beta Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-beta Tubulin antibody
ICC/IF image of ab15568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Beta Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-beta Tubulin antibody
ab15568 staining beta Tubulin in IMR32 Human neuroblastoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA containing TBST) for 16 hours at 4°C. A Cy3®-conjugated Goat anti-rabbit IgG polyclonal (1:1000) was used as the secondary antibody. Blue - nuclei (DAPI).
Beta Tubulin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-beta Tubulin antibody
ab15568 staining beta Tubulin in Human lung by Immunohistochemistry (FFPE-sections).
Beta Tubulin Western blot staining using rabbit Anti-beta Tubulin antibody
All lanes: Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568) at 1 µg/mL
All lanes: Western blot - Recombinant Human beta Tubulin protein (Recombinant Human beta Tubulin protein ab70187) at 0.1 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Exposure time: 30s
Beta Tubulin Western blot staining using rabbit Anti-beta Tubulin antibody
All lanes: Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568) at 1 µg/mL
All lanes: Western blot - Recombinant Human beta Tubulin protein (Recombinant Human beta Tubulin protein ab70187) at 0.1 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Exposure time: 30s
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