AB15568

Anti-beta Tubulin antibody - Loading Control

Name: Anti-beta Tubulin antibody - Loading Control (ab15568) | Abcam
Brand: Abcam Limited
SKU: AB15568
Price: 410 USD
Availability: InStock
Rating: 5 (6 reviews)

(6 Reviews)

(128 Publications)

Anti-beta Tubulin antibody (ab15568) is a rabbit polyclonal antibody detecting beta Tubulin in Western Blot, IHC-P, ICC/IF. Suitable for Human.

- Over 100 publications
- Trusted since 2004

View Alternative Names

TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain

6 Images

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Western blot - Anti-beta Tubulin antibody - Loading Control (AB15568)

All lanes:

Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 49 kDa

Observed band size: 55 kDa

false

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (AB15568)

ICC/IF image of ab15568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody - Loading Control (AB15568)

ab15568 staining beta Tubulin in Human lung by Immunohistochemistry (FFPE-sections).

ICC/IF

AbReview39298****

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (AB15568)

ab15568 staining beta Tubulin in IMR32 Human neuroblastoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA containing TBST) for 16 hours at 4°C. A Cy3®-conjugated Goat anti-rabbit IgG polyclonal (1 : 1000) was used as the secondary antibody. Blue - nuclei (DAPI).

This image is courtesy of an anonymous Abreview

Unknown

Western blot - Anti-beta Tubulin antibody - Loading Control (AB15568)

All lanes:

Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568) at 1 µg/mL

All lanes:

Western blot - Recombinant Human beta Tubulin protein (<a href='/en-us/products/proteins-peptides/recombinant-human-beta-tubulin-protein-ab70187'>ab70187</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 49 kDa

true

Exposure time: 30s

Unknown

Western blot - Anti-beta Tubulin antibody - Loading Control (AB15568)

All lanes:

Western blot - Anti-beta Tubulin antibody - Loading Control (ab15568) at 1 µg/mL

All lanes:

Western blot - Recombinant Human beta Tubulin protein (<a href='/en-us/products/proteins-peptides/recombinant-human-beta-tubulin-protein-ab70187'>ab70187</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 49 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Homology with Tubulin beta chain and Tubulin beta-3 chain

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": " Antigen retrieval is not essential but may optimise staining.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Fungi": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rice": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Saccharomyces cerevisiae": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sea urchin": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-beta Tubulin antibody (ab15568) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of beta Tubulin?
Anti-beta Tubulin (ab15568) specifically detects a band for beta Tubulin (UniProt: Q13509) at a molecular weight of 55kDa.

Trusted by the scientific community
Anti-beta Tubulin (ab15568) was first used in a scientific publication in 2004 and has been cited over 100 times in peer-reviewed journals.

Reviewed by scientists
Anti-beta Tubulin (ab15568) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta tubulin also known as beta-tubulin or Β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB

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Publications (128)

Recent publications for all applications. Explore the full list and refine your search

ACS omega 10:39693-39705 PubMed40949268

2025

Biodegradable Nanofiber Membranes for Air-Liquid Interface Culture: Advancing Airway Models.

Applications

Unspecified application

Species

Unspecified reactive species

Sema Tuncer,Secil Subasi Can,Hayriye Akel Bilgic,Busra Kilic,Gulcin Gunal,Halil Murat Aydin,Cagatay Karaaslan

Circulation journal : official journal of the Japanese Circulation Society 89:1245-1255 PubMed40368806

2025

Circulating HMGB2 Is Associated With Angiographic Coronary Collateralization in Diabetic Patients With Chronic Total Occlusion and Impairs Vessel Growth via NLRP3.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Ming Wu,Shuai Chen,Yang Dai,Ke Huang,Xiao Qun Wang,Lin Shuang Mao,Chen Die Yang,Fei Fei Li,Le Ying Li,Jing Meng Liu,Yi Xuan Wang,Wen Jia Li,Ruo Sen Yuan,Lin Lu,Rui Yan Zhang,Wei Feng Shen,Ying Shen,Feng Hua Ding

International journal of molecular sciences 26: PubMed40244179

2025

Mating Increases CHST10 Activity in Rat Oviductal Mucosa to Induce the Synthesis of HNK-1 Glycoproteins: Possible Role in Sperm-Oviduct Interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Francisca Fábrega-Guerén,Juan C Andrade,Marlene Zúñiga-Cóndor,Patricio Morales,Benito Gómez-Silva,Lidia M Zúñiga

PLoS pathogens 21:e1012789 PubMed39752360

2025

Small interfering RNAs generated from the terminal panhandle structure of negative-strand RNA virus promote viral infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wan Zhao,Qiong Li,Mengqi Sun,Lan Luo,Xiaoming Zhang,Feng Cui

The Journal of biological chemistry 301:108065 PubMed39662833

2024

Structural and functional properties of the N- and C-terminal segments of the P4-ATPase phospholipid flippase ATP8A2.

Applications

Unspecified application

Species

Unspecified reactive species

Eli Matsell,Moloud Mazaheri,Jens Peter Andersen,Robert S Molday

Nature communications 15:10496 PubMed39627214

2024

ATAD5-BAZ1B interaction modulates PCNA ubiquitination during DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yeongjae Kim,Na Young Ha,Mi-Sun Kang,Eunjin Ryu,Geunil Yi,Juyeong Yoo,Nalae Kang,Byung-Gyu Kim,Kyungjae Myung,Sukhyun Kang

International journal of molecular sciences 25: PubMed39409130

2024

Thyroid Hormone Upregulates Cav1.2 Channels in Cardiac Cells via the Downregulation of the Channels' β4 Subunit.

Applications

Unspecified application

Species

Unspecified reactive species

Elba D Carrillo,Juan A Alvarado,Ascención Hernández,Ivonne Lezama,María C García,Jorge A Sánchez

Heliyon 10:e34834 PubMed39149033

2024

AUY922 improves sensitivity to sunitinib in clear cell renal cell carcinoma based on network pharmacology and in vitro experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Zixuan Chen,Xing Jia,Yuesong Cai,Ya Song,Yanjun Tong,Sheng Cheng,Min Liu

Nature communications 15:5448 PubMed38937444

2024

Distinct functions of microtubules and actin filaments in the transportation of the male germ unit in pollen.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangfei Wang,Tonghui Li,Jiuting Xu,Fanfan Zhang,Lifang Liu,Ting Wang,Chun Wang,Haiyun Ren,Yi Zhang

International journal of molecular sciences 25: PubMed38791456

2024

Presenilin Deficiency Results in Cellular Cholesterol Accumulation by Impairment of Protein Glycosylation and NPC1 Function.

Applications

Unspecified application

Species

Unspecified reactive species

Marietta Fabiano,Naoto Oikawa,Anja Kerksiek,Jun-Ichi Furukawa,Hirokazu Yagi,Koichi Kato,Ulrich Schweizer,Wim Annaert,Jongkyun Kang,Jie Shen,Dieter Lütjohann,Jochen Walter

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta Tubulin antibody - Loading Control

Western blot - Anti-beta Tubulin antibody - Loading Control (AB15568)

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (AB15568)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody - Loading Control (AB15568)

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody - Loading Control (AB15568)

Western blot - Anti-beta Tubulin antibody - Loading Control (AB15568)

Western blot - Anti-beta Tubulin antibody - Loading Control (AB15568)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (128)

Product promise