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AB224612

Anti-BHC80 / PHF21A antibody

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(2 Publications)

Rabbit Polyclonal BHC80 / PHF21A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PHF21A aa 300-500.

View Alternative Names

BHC80, KIAA1696, BM-006, PHF21A, PHD finger protein 21A, BHC80a, BRAF35-HDAC complex protein BHC80

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-BHC80 / PHF21A antibody (AB224612)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BHC80 / PHF21A antibody (AB224612)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for BHC80 / PHF21A (green) using ab224612 (1/100 dilution) in ICC/IF. Secondary : Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHC80 / PHF21A antibody (AB224612)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHC80 / PHF21A antibody (AB224612)

Paraffin embedded human adrenal gland tissue stained for BHC80 / PHF21A with ab224612 (1/100 dilution) in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHC80 / PHF21A antibody (AB224612)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHC80 / PHF21A antibody (AB224612)

Paraffin embedded human liver cancer tissue stained for BHC80 / PHF21A with ab224612 (1/100 dilution) in immunohistochemical analysis.

Western blot - Anti-BHC80 / PHF21A antibody (AB224612)
  • WB

Supplier Data

Western blot - Anti-BHC80 / PHF21A antibody (AB224612)

All lanes:

Western blot - Anti-BHC80 / PHF21A antibody (ab224612) at 1/500 dilution

Lane 1:

Mouse liver tissue

Lane 2:

Mouse kidney tissue

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PHF21A aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

Q96BD5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation.
See full target information PHF21A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Neoplasma 69:1349-1358 PubMed36305691

2022

PHF21A expression as a biomarker of hepatocellular carcinoma progression and prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Qing Huang,Li-Chen Ji,Qiang-An Jing,Yi-Chen He,Ying-Yu Ma,Xiang-Min Tong

Bioengineered 12:10073-10088 PubMed34709988

2021

Screening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liru Zhang,Huanju Li,Yongle Qiu,Yuanhang Liu,Xin Liu,Wenjing Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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