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AB282156

Anti-BHMT antibody [3D6]

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(1 Publication)

Mouse Recombinant Monoclonal BHMT antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Betaine--homocysteine S-methyltransferase 1.

View Alternative Names

Betaine--homocysteine S-methyltransferase 1, BHMT

3 Images
Flow Cytometry (Intracellular) - Anti-BHMT antibody [3D6] (AB282156)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BHMT antibody [3D6] (AB282156)

Flow cytometry analysis of Hep3B cells labelling BHMT with ab282156 at 2-5ug for 1x106 cells (red). A Goat anti-mouse IgG (Alexa Fluor® 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Immunocytochemistry/ Immunofluorescence - Anti-BHMT antibody [3D6] (AB282156)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BHMT antibody [3D6] (AB282156)

Immunocytochemical analysis of Hep3B cells labelling BHMT with ab282156 at 1/100 dilution followed by Alexa Fluor® 488 secondary antibody (green). DAPI was stained the cell nucleus (blue).

Western blot - Anti-BHMT antibody [3D6] (AB282156)
  • WB

Supplier Data

Western blot - Anti-BHMT antibody [3D6] (AB282156)

SDS-PAGE gel was transferred to PVDF membrane.

ECL detection system.

All lanes:

Western blot - Anti-BHMT antibody [3D6] (ab282156) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate at 40 µg

Secondary

All lanes:

Goat anti-Mouse secondary antibody HRP-conjugate

Predicted band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3D6

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Betaine--homocysteine S-methyltransferase 1.

Q93088

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "2-5 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 10% Glycerol (glycerin, glycerine), 0.75% Sodium chloride, 0.13% Disodium hydrogenorthophosphate, 0.02% Potassium chloride, 0.02% Potassium phosphate monobasic
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
See full target information Betaine--homocysteine S-methyltransferase 1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine : PubMed40603105

2025

KLF16-Induced Increase of GARS Promotes Glycolysis in Hepatocellular Carcinoma by Stabilizing FOXK1.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Wang,Ruijuan Fan,Jun Li,Hanbo Qiu,Lizhu Lin
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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