Mouse Recombinant Monoclonal BHMT antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra), Flow Cyt and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Betaine--homocysteine S-methyltransferase 1.
Images
![Western blot - Anti-BHMT antibody [3D6] (AB282156), expandable thumbnail](https://content.abcam.com/products/images/bhmt-antibody-3d6-ab282156--western-blot-img184114.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BHMT antibody [3D6] (AB282156)")
![Flow Cytometry - Anti-BHMT antibody [3D6] (AB282156), expandable thumbnail](https://content.abcam.com/products/images/bhmt-antibody-3d6-ab282156--flow-cytometry-img179987.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-BHMT antibody [3D6] (AB282156)")
![Immunocytochemistry/ Immunofluorescence - Anti-BHMT antibody [3D6] (AB282156), expandable thumbnail](https://content.abcam.com/products/images/bhmt-antibody-3d6-ab282156--immunocytochemistry-immunofluorescence-img168791.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BHMT antibody [3D6] (AB282156)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 10% Glycerol (glycerin, glycerine), 0.75% Sodium chloride, 0.13% Disodium hydrogenorthophosphate, 0.02% Potassium chloride, 0.02% Potassium phosphate monobasic- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human Betaine--homocysteine S-methyltransferase 1. Database link Q93088
Abcam Recommends
Reactivity data
Select an application| ICC/IF | WB | Flow Cyt (Intra) | Flow Cyt | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Tested |
| Mouse | Expected | Tested | Expected | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2-5 µg for 106 Cells | Notes - |
Species Human | Dilution info 2-5 µg for 106 Cells | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-5 µg for 106 Cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Alternative names
Betaine--homocysteine S-methyltransferase 1, BHMT
Recommended products
Mouse Recombinant Monoclonal BHMT antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra), Flow Cyt and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Betaine--homocysteine S-methyltransferase 1.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 10% Glycerol (glycerin, glycerine), 0.75% Sodium chloride, 0.13% Disodium hydrogenorthophosphate, 0.02% Potassium chloride, 0.02% Potassium phosphate monobasic- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human Betaine--homocysteine S-methyltransferase 1. Database link Q93088
- Clone number
- 3D6
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-BHMT antibody [3D6] (ab282156), expandable thumbnail](https://content.abcam.com/products/images/bhmt-antibody-3d6-ab282156--western-blot-img184114.jpg "Western blot - Anti-BHMT antibody [3D6] (ab282156)")
Western blot - Anti-BHMT antibody [3D6] (ab282156)
SDS-PAGE gel was transferred to PVDF membrane.
ECL detection system.
All lanes: Western blot - Anti-BHMT antibody [3D6] (ab282156) at 1/1000 dilution
All lanes: Mouse liver tissue lysate at 40 µg
SecondaryAll lanes: Goat anti-Mouse secondary antibody HRP-conjugate
Predicted band size: 45 kDa
![Flow Cytometry - Anti-BHMT antibody [3D6] (ab282156), expandable thumbnail](https://content.abcam.com/products/images/bhmt-antibody-3d6-ab282156--flow-cytometry-img179987.jpg "Flow Cytometry - Anti-BHMT antibody [3D6] (ab282156)")
Flow Cytometry - Anti-BHMT antibody [3D6] (ab282156)
Flow cytometry analysis of Hep3B cells labelling BHMT with ab282156 at 2-5ug for 1x106 cells (red). A Goat anti-mouse IgG (Alexa Fluor® 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
![Immunocytochemistry/ Immunofluorescence - Anti-BHMT antibody [3D6] (ab282156), expandable thumbnail](https://content.abcam.com/products/images/bhmt-antibody-3d6-ab282156--immunocytochemistry-immunofluorescence-img168791.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BHMT antibody [3D6] (ab282156)")
Immunocytochemistry/ Immunofluorescence - Anti-BHMT antibody [3D6] (ab282156)
Immunocytochemical analysis of Hep3B cells labelling BHMT with ab282156 at 1/100 dilution followed by Alexa Fluor® 488 secondary antibody (green). DAPI was stained the cell nucleus (blue).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com