Rabbit Polyclonal BHMT antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Betaine--homocysteine S-methyltransferase 1 aa 150-400.
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Publications
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- Hepatology (Baltimore, Md.) 75:567-5832021Intrahepatic cholangiocyte regeneration from an Fgf-dependent extrahepatic progenitor niche in a zebrafish model of Alagille Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengjian Zhao,Joseph J Lancman,Yi Yang,Keith P Gates,Dan Cao,Lindsey Barske,Jonathan Matalonga,Xiangyu Pan,Jiaye He,Alyssa Graves,Jan Huisken,Chong Chen,P Duc Si DongPubMed 34569629
- Molecular psychiatry 25:2620-26292019Maternal choline supplementation ameliorates Alzheimer's disease pathology by reducing brain homocysteine levels across multiple generations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRamon Velazquez et. al.PubMed 30622336
- Biochemical and biophysical research communication 458:626-6312015Isoaspartate, carbamoyl phosphate synthase-1, and carbonic anhydrase-III as biomarkers of liver injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWayne G Carter et. al.PubMed 25684186
- The British journal of nutrition 112:1274-852014Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarit Espe et. al.PubMed 25196630
- PloS one 8:e856322013Homocysteine homeostasis and betaine-homocysteine S-methyltransferase expression in the brain of hibernating bats.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Betaine--homocysteine S-methyltransferase 1 aa 150-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q93088
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Expected | Expected |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Antigen retrieval is not essential but may optimise staining. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Alternative names
Betaine--homocysteine S-methyltransferase 1, BHMT
Recommended products
Rabbit Polyclonal BHMT antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Betaine--homocysteine S-methyltransferase 1 aa 150-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Betaine--homocysteine S-methyltransferase 1 aa 150-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q93088
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-BHMT antibody (ab96415)
10% SDS PAGE
All lanes: Western blot - Anti-BHMT antibody (ab96415) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT antibody (ab96415)
Immunohistochemical analysis of paraffin-embedded human SG xenograft, using ab96415 at 1/100 dilution.
Western blot - Anti-BHMT antibody (ab96415)
All lanes: Western blot - Anti-BHMT antibody (ab96415) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 50 µg
Lane 2: Mouse lung tissue lysate at 50 µg
Lane 3: Mouse heart tissue lysate at 50 µg
Predicted band size: 45 kDa
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