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Rabbit Recombinant Monoclonal BHMT antibody. Suitable for WB, IHC-P and reacts with Rat, Human, Recombinant fragment samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (AB213491), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (AB213491), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (AB213491), expandable thumbnail
  • Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (AB213491), expandable thumbnail
  • Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (AB213491), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Rat
Tested
Tested
Recombinant fragment
Expected
Tested

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Recombinant fragment
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Recombinant fragment
Dilution info
1/20000
Notes

IHC note: 1:20,000, recommend human and rat. BHMT2 involved in the regulation of homocysteine metabolism. It's mainly expressed on liver and kidney and its subcellular location was cytoplasm. IHC results showed specific staining on liver and kidney of human and liver, mouse kidney was very weak staining and testis leydig cells were non-specific staining. So IHC recommend human and rat.

Note: It can show nuclear staining if working at higher concentration.

Species
Rat
Dilution info
1/20000
Notes

IHC note: 1:20,000, recommend human and rat. BHMT2 involved in the regulation of homocysteine metabolism. It's mainly expressed on liver and kidney and its subcellular location was cytoplasm. IHC results showed specific staining on liver and kidney of human and liver, mouse kidney was very weak staining and testis leydig cells were non-specific staining. So IHC recommend human and rat.

Note: It can show nuclear staining if working at higher concentration.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/20000
Notes

IHC note: 1:20,000, recommend human and rat. BHMT2 involved in the regulation of homocysteine metabolism. It's mainly expressed on liver and kidney and its subcellular location was cytoplasm. IHC results showed specific staining on liver and kidney of human and liver, mouse kidney was very weak staining and testis leydig cells were non-specific staining. So IHC recommend human and rat.

Note: It can show nuclear staining if working at higher concentration.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Additional Targets

S-methylmethionine--homocysteine S-methyltransferase BHMT2

Alternative names

Betaine--homocysteine S-methyltransferase 1, BHMT

Recommended products

Rabbit Recombinant Monoclonal BHMT antibody. Suitable for WB, IHC-P and reacts with Rat, Human, Recombinant fragment samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20822
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling BHMT + BHMT2 with ab213491 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human kidney tissue is observed (PMID: 9281325; PMID: 26592251). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    IHC note: 1:20,000, recommend human and rat. BHMT2 involved in the regulation of homocysteine metabolism. It's mainly expressed on liver and kidney and its subcellular location was cytoplasm. IHC results showed specific staining on liver and kidney of human and liver, mouse kidney was very weak staining and testis leydig cells were non-specific staining. So IHC recommend human and rat.

    Note: It can show nuclear staining if working at higher concentration.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BHMT + BHMT2 with ab213491 at a 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human liver is observed (PMID: 9281325; PMID: 26592251). Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling BHMT + BHMT2 with ab213491 at 1/20,000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on rat liver is observed (PMID: 9281325; PMID: 26592251).counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491), expandable thumbnail

    Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Lane 1 and 2 samples loaded at 10 ng.

    Exposure time: 58 seconds.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    All lanes: Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491) at 1/1000 dilution

    Lane 1: Western blot - Recombinant Human BHMT2 protein (denatured) (Recombinant Human BHMT2 protein (denatured) ab180277)

    Lane 2: Western blot - Recombinant Human BHMT protein (Recombinant Human BHMT protein ab51280)

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  • Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491), expandable thumbnail

    Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 minutes

    BHMT and BHMT2 are mainly expressed in liver and kidney, negative in spleen and other tissues (PMID18457970; PMID: 11087663). The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    All lanes: Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491) at 1/1000 dilution

    Lane 1: Human fetal liver tissue at 20 µg

    Lane 2: Human fetal kidney tissue at 20 µg

    Lane 3: Human fetal spleen tissue at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  • Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491), expandable thumbnail

    Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 7 seconds.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    All lanes: Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (ab213491) at 1/1000 dilution

    All lanes: Rat liver tissue at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 45 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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