AB117818

Anti-BICD2 antibody

Name: Anti-BICD2 antibody (ab117818) | Abcam
Brand: Abcam Limited
SKU: AB117818
Price: 525 USD
Availability: InStock

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(14 Publications)

Rabbit Polyclonal BICD2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human BICD2 aa 350-450.

View Alternative Names

KIAA0699, BICD2, Protein bicaudal D homolog 2, Bic-D 2

2 Images

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Immunoprecipitation - Anti-BICD2 antibody (AB117818)

Detection of Human BICD2 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells using ab117818 at 6 µg/mg of lysate. ab117818 at 0.4 µg/ml was used for subsequent WB detection of the immunoprecipitate.
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-BICD2 antibody (ab117818)

Predicted band size: 94 kDa

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Unknown

Western blot - Anti-BICD2 antibody (AB117818)

All lanes:

Western blot - Anti-BICD2 antibody (ab117818) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 94 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human BICD2 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

Q8TD16

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab117818 was affinity purified using an epitope specific to BICD2 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates and stabilizes the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed : 25814576). Facilitates the binding of RAB6A to the Golgi by stabilizing its GTP-bound form. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport via its interaction with RAB6A and recruitment of the dynein-dynactin motor complex (PubMed : 25962623). Contributes to nuclear and centrosomal positioning prior to mitotic entry through regulation of both dynein and kinesin-1. During G2 phase of the cell cycle, associates with RANBP2 at the nuclear pores and recruits dynein and dynactin to the nuclear envelope to ensure proper positioning of the nucleus relative to centrosomes prior to the onset of mitosis (By similarity).

See full target information BICD2

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0281124 PubMed39431827

2024

Rab6a enables BICD2/dynein-mediated trafficking of human papillomavirus from the Golgi network during virus entry.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongjoon Choi,Kaitlyn Speckhart,Billy Tsai,Daniel DiMaio

Communications biology 7:1305 PubMed39394274

2024

KIF1C facilitates retrograde transport of lysosomes through Hook3 and dynein.

Applications

Unspecified application

Species

Unspecified reactive species

Takeshi Saji,Mitsuharu Endo,Yasushi Okada,Yasuhiro Minami,Michiru Nishita

The Journal of cell biology 223: PubMed39115447

2024

Nesprin-2 coordinates opposing microtubule motors during nuclear migration in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Chuying Zhou,You Kure Wu,Fumiyoshi Ishidate,Takahiro K Fujiwara,Mineko Kengaku

PLoS pathogens 20:e1012289 PubMed38829892

2024

The BICD2 dynein cargo adaptor binds to the HPV16 L2 capsid protein and promotes HPV infection.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitlyn Speckhart,Jeongjoon Choi,Daniel DiMaio,Billy Tsai

Nature communications 14:2434 PubMed37105961

2023

BICD2 phosphorylation regulates dynein function and centrosome separation in G2 and M.

Applications

Unspecified application

Species

Unspecified reactive species

Núria Gallisà-Suñé,Paula Sànchez-Fernàndez-de-Landa,Fabian Zimmermann,Marina Serna,Laura Regué,Joel Paz,Oscar Llorca,Jens Lüders,Joan Roig

PLoS pathogens 18:e1010824 PubMed36067270

2022

Components of the LINC and NPC complexes coordinately target and translocate a virus into the nucleus to promote infection.

Applications

Unspecified application

Species

Unspecified reactive species

Chelsey C Spriggs,Grace Cha,Jiaqian Li,Billy Tsai

The Journal of cell biology 220: PubMed34709360

2021

Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.

Applications

Unspecified application

Species

Unspecified reactive species

Amrita Kumari,Chandan Kumar,Rajaiah Pergu,Megha Kumar,Sagar P Mahale,Neeraj Wasnik,Sivaram V S Mylavarapu

Acta neuropathologica communications 8:106 PubMed32665036

2020

Impairment in dynein-mediated nuclear translocation by BICD2 C-terminal truncation leads to neuronal migration defect and human brain malformation.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Han Tsai,Haw-Yuan Cheng,Fang-Shin Nian,Chen Liu,Nian-Hsin Chao,Kuo-Liang Chiang,Shu-Fang Chen,Jin-Wu Tsai

Current biology : CB 30:3116-3129.e4 PubMed32619477

2020

Nesprin-2 Recruitment of BicD2 to the Nuclear Envelope Controls Dynein/Kinesin-Mediated Neuronal Migration In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

João Carlos Gonçalves,Sebastian Quintremil,Julie Yi,Richard B Vallee

Developmental cell 53:141-153.e4 PubMed32275887

2020

The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport, and Clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Noopur V Khobrekar,Sebastian Quintremil,Tiago J Dantas,Richard B Vallee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-BICD2 antibody

Immunoprecipitation - Anti-BICD2 antibody (AB117818)

Western blot - Anti-BICD2 antibody (AB117818)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (14)

Product promise