Anti-BICD2 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BICD2 antibody (AB237616)

Paraffin-embedded cervical cancer tissue stained for BICD2 using ab237616 at 1/500 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-BICD2 antibody (AB237616)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for BICD2 (green) using ab237616 at 1/166 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The nuclear counter stain is DAPI.

• WB

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Western blot - Anti-BICD2 antibody (AB237616)

All lanes:

Western blot - Anti-BICD2 antibody (ab237616) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lane 3:

HL-60 (human promyelocytic leukemia cell line) whole cell lysate

Lane 4:

A549 (human lung carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 94 kDa

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