AB272880

Anti-Bid antibody

Name: Anti-Bid antibody (ab272880) | Abcam
Brand: Abcam Limited
SKU: AB272880

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(5 Publications)

Rabbit Polyclonal BID antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BID.

View Alternative Names

BH3-interacting domain death agonist, p22 BID, BID

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Bid antibody (AB272880)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bid antibody (AB272880)

4% PFA-fixed A431 (human epidermoid carcinoma cell line) cells stained for Bid using ab272880 at 1/1000 dilution in ICC/IF.

Red : alpha Tubulin, a cytoskeleton marker.

Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bid antibody (AB272880)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bid antibody (AB272880)

Paraffin-embedded U87 xenograft tissue stained for Bid using ab272880 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Bid antibody (AB272880)

2% paraformaldehyde/culture medium fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Bid using ab272880 at 1/1000 dilution in ICC/IF.

Blue : Hoechst 33343.

Unknown

Western blot - Anti-Bid antibody (AB272880)

12% SDS-PAGE

All lanes:

Western blot - Anti-Bid antibody (ab272880) at 1/500 dilution

Lane 1:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

Lane 2:

C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

Predicted band size: 22 kDa

false

Unknown

Western blot - Anti-Bid antibody (AB272880)

12% SDS-PAGE

All lanes:

Western blot - Anti-Bid antibody (ab272880) at 1/1000 dilution

All lanes:

Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg

Predicted band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human BID. The exact immunogen used to generate this antibody is proprietary information.

P55957

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bid also known as BH3-interacting domain death agonist or BID protein functions as a pro-apoptotic member of the Bcl-2 protein family. This protein plays a significant role in the apoptotic process by helping to mediate the release of cytochrome c from mitochondria an important step in this cell death pathway. Bid exists in cells as an inactive uncleaved form but upon activation a caspase cleaves it into a truncated form tBID. The molecular weight of Bid is approximately 22 kDa. It is commonly expressed in numerous tissues throughout the body contributing to its various apoptotic regulatory roles.

Biological function summary

Bid induces mitochondria-mediated apoptosis that significantly affects cellular homeostasis. Bid is not considered to be part of a permanent protein complex; instead it acts as a mobile activator bridging interactions between different proteins within the apoptotic pathway. Upon activation tBID interacts with other Bcl-2 family members like Bax and Bak which leads to mitochondrial membrane permeabilization and subsequent release of cytochrome c.

Pathways

Bid significantly influences apoptotic signaling under different cellular stress conditions. It plays important functions in both intrinsic and extrinsic pathways of apoptosis. Within the intrinsic pathway Bid links the external death receptor signals to the mitochondria-mediated apoptotic pathway. In addition to interacting with Bax and Bak Bid indirectly connects to the apoptosome complex facilitating caspase activation which ultimately results in cellular apoptosis.

Bid has strong associations with conditions characterized by deregulated apoptosis such as cancer and neurodegenerative diseases. For instance downregulation or dysfunction of Bid is noted in certain cancer types contributing to the evasion of apoptosis a hallmark of cancer. Moreover in neurodegenerative diseases where excess apoptosis leads to loss of neuronal cells Bid activity has shown to connect with the regulation of apoptosis through its interactions with key apoptotic proteins such as Bcl-2 and Bax. Understanding Bid's role provides potential therapeutic targets to either promote or inhibit apoptotic processes in disease treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Induces caspases and apoptosis (PubMed : 14583606). Counters the protective effect of BCL2 (By similarity).. BH3-interacting domain death agonist p15. Induces caspase activation and apoptosis (PubMed : 15661737, PubMed : 32029622). Translocates to mitochondria following cleavage and disrupts the outer mitochondrial membrane leading to the release of cytochrome c (PubMed : 10480954, PubMed : 32029622, PubMed : 9727491).. Isoform 1. Induces ICE-like proteases and apoptosis.. Isoform 2. Induces ICE-like proteases and apoptosis.. Isoform 3. Does not induce apoptosis.. Isoform 4. Induces ICE-like proteases and apoptosis.

See full target information BID

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3003 PubMed40148277

2025

Proapoptotic Bcl-2 inhibitor as potential host directed therapy for pulmonary tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Medha Singh,Mona O Sarhan,Nerketa N L Damiba,Alok K Singh,Andres Villabona-Rueda,Oscar J Nino-Meza,Xueyi Chen,Yuderleys Masias-Leon,Carlos E Ruiz-Gonzalez,Alvaro A Ordonez,Franco R D'Alessio,Eric O Aboagye,Laurence S Carroll,Sanjay K Jain

Communications biology 6:365 PubMed37012358

2023

LncAABR07053481 inhibits bone marrow mesenchymal stem cell apoptosis and promotes repair following steroid-induced avascular necrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Zhi-Hong Xie,Lei Wang,Hong Luo,Jian Zhang,Wen-Tao Dong,Xiao-Han Zheng,Chuan Ye,Xiao-Bin Tian,Gang Liu,Xue-Song Zhu,Yan-Lin Li,Qing-Lin Kang,Fei Zhang,Wu-Xun Peng

Cancers 13: PubMed34572804

2021

The Stress-Inducible BCL2A1 Is Required for Ovarian Cancer Metastatic Progression in the Peritoneal Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liang,Mingo M H Yung,Fangfang He,Peili Jiao,Karen K L Chan,Hextan Y S Ngan,David W Chan

Drug development research 82:1131-1143 PubMed33818788

2021

CircFAT1 facilitates cervical cancer malignant progression by regulating ERK1/2 and p38 MAPK pathway through miR-409-3p/CDK8 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Zhou,Ting Li,Rongping Xie,Jianbin Zhou,Jue Liu,Yu Luo,Xia Zhang

Cell proliferation 54:e13022 PubMed33686740

2021

SCARF1 promotes M2 polarization of Kupffer cells via calcium-dependent PI3K-AKT-STAT3 signalling to improve liver transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Song Xu,Zhi-Hao Feng,Ding Cao,Hao Wu,Meng-Hao Wang,Jin-Zheng Li,Jian-Ping Gong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bid antibody

Immunocytochemistry/ Immunofluorescence - Anti-Bid antibody (AB272880)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bid antibody (AB272880)

Immunocytochemistry/ Immunofluorescence - Anti-Bid antibody (AB272880)

Western blot - Anti-Bid antibody (AB272880)

Western blot - Anti-Bid antibody (AB272880)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise