AB317809

Anti-Bid antibody [EPR27444-102]

20ul selling size
Recombinant
RabMAb
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Rabbit Recombinant Monoclonal BID antibody. Suitable for WB and reacts with Mouse samples.

View Alternative Names

BH3-interacting domain death agonist, p22 BID, BID, Bid

2 Images

Supplier Data

Western blot - Anti-Bid antibody [EPR27444-102] (AB317809)

The expression of Bid is downregulated in response to TNF alpha and BV-6 treatment (PMID : 11462023).

The identity of the higher MW bands at approximately 250 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Bid antibody [EPR27444-102] (ab317809) at 1/1000 dilution

Lane 1:

Untreated L-929 (mouse connective tissue fibroblast) whole cell lysate at 50 µg

Lane 2:

L-929 treated with 20ng/ml TNF alpha and 10µM BV-6 for 10h whole cell lysate at 50 µg

Lane 3:

C2C12 (mouse myoblast) whole cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 22 kDa,36 kDa

false

Exposure time: 6s

Supplier Data

Western blot - Anti-Bid antibody [EPR27444-102] (AB317809)

Low expression : mouse lung (PMID : 22446738).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Bid antibody [EPR27444-102] (ab317809) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 50 µg

Lane 2:

Mouse thymus tissue lysate at 50 µg

Lane 3:

Mouse lung tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 22 kDa,36 kDa

false

Exposure time: 6s

Carrier free

Anti-Bid antibody [EPR27444-102] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27444-102

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bid also known as BH3-interacting domain death agonist or BID protein functions as a pro-apoptotic member of the Bcl-2 protein family. This protein plays a significant role in the apoptotic process by helping to mediate the release of cytochrome c from mitochondria an important step in this cell death pathway. Bid exists in cells as an inactive uncleaved form but upon activation a caspase cleaves it into a truncated form tBID. The molecular weight of Bid is approximately 22 kDa. It is commonly expressed in numerous tissues throughout the body contributing to its various apoptotic regulatory roles.

Biological function summary

Bid induces mitochondria-mediated apoptosis that significantly affects cellular homeostasis. Bid is not considered to be part of a permanent protein complex; instead it acts as a mobile activator bridging interactions between different proteins within the apoptotic pathway. Upon activation tBID interacts with other Bcl-2 family members like Bax and Bak which leads to mitochondrial membrane permeabilization and subsequent release of cytochrome c.

Pathways

Bid significantly influences apoptotic signaling under different cellular stress conditions. It plays important functions in both intrinsic and extrinsic pathways of apoptosis. Within the intrinsic pathway Bid links the external death receptor signals to the mitochondria-mediated apoptotic pathway. In addition to interacting with Bax and Bak Bid indirectly connects to the apoptosome complex facilitating caspase activation which ultimately results in cellular apoptosis.

Bid has strong associations with conditions characterized by deregulated apoptosis such as cancer and neurodegenerative diseases. For instance downregulation or dysfunction of Bid is noted in certain cancer types contributing to the evasion of apoptosis a hallmark of cancer. Moreover in neurodegenerative diseases where excess apoptosis leads to loss of neuronal cells Bid activity has shown to connect with the regulation of apoptosis through its interactions with key apoptotic proteins such as Bcl-2 and Bax. Understanding Bid's role provides potential therapeutic targets to either promote or inhibit apoptotic processes in disease treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Induces caspases and apoptosis. Counters the protective effect of BCL2.. BH3-interacting domain death agonist p15. Induces caspase activation and apoptosis (By similarity). Allows the release of cytochrome c (PubMed : 9873064).

See full target information Bid

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bid antibody [EPR27444-102]

Western blot - Anti-Bid antibody [EPR27444-102] (AB317809)

Western blot - Anti-Bid antibody [EPR27444-102] (AB317809)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise