AB10640

Anti-Bid Cleavage Site antibody

Name: Anti-Bid Cleavage Site antibody (ab10640) | Abcam
Brand: Abcam Limited
SKU: AB10640
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(21 Publications)

Rabbit Polyclonal BID antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Mouse Bid.

View Alternative Names

BH3-interacting domain death agonist, p22 BID, BID, Bid

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Bid Cleavage Site antibody (AB10640)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bid Cleavage Site antibody (AB10640)

A549 cells stained for BID cleave site (green) using ab10640 at 1/250 dilution in ICC/IF. It was followed by Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at 1/400 dilution for 30 minutes at room temperature (Panel a). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant DAPI. F-actin (Panel c : red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e shows no primary antibody control.

Supplier Data

Western blot - Anti-Bid Cleavage Site antibody (AB10640)

SDS-PAGE on a 4-20% Tris-glycine gel

All lanes:

Western blot - Anti-Bid Cleavage Site antibody (ab10640) at 1/1000 dilution

Lane 1:

3T3-L1 cells without BID

Lane 2:

3T3-L1 cells with caspase-8 cleaved recombinant mouse BID

Lane 3:

3T3-L1 cells with caspase-8 cleaved recombinant human BID

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Mouse Bid. The exact immunogen used to generate this antibody is proprietary information.

P70444

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from rabbit serum by epitope-specific affinity chromatography.

Storage buffer

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bid Cleavage Site is a region on the Bid protein which is also known as BH3 interacting domain death agonist. This protein is part of the Bcl-2 family and acts as a pro-apoptotic member. The molecular weight of the full-length Bid protein is approximately 22 kDa. Bid is expressed in various tissues within the body including the heart and liver. During apoptosis caspases cleave Bid resulting in a truncated form called tBid which significantly enhances its pro-apoptotic activity.

Biological function summary

Bid functions as a critical mediator of apoptosis. It acts within the intrinsic pathway a vital part of programmed cell death. Bid does not function as a monomer but can form complexes with other Bcl-2 family proteins such as Bax and Bak promoting mitochondrial outer membrane permeabilization. Upon cleavage tBid translocates to the mitochondria to facilitate the release of cytochrome c initiating the cascade of apoptotic events.

Pathways

Bid plays an integral role in the apoptotic signaling pathways. The intrinsic or mitochondrial pathway is the primary context in which Bid operates. This pathway involves several key proteins including Bax and Bak which cooperate with tBid to permeabilize the mitochondrial membrane. In addition to its role in apoptosis Bid is indirectly linked with the extrinsic apoptotic pathway through its interaction with caspase-8 which also induces its cleavage into tBid.

Bid has shown significant implications in cancer and neurodegenerative diseases. In cancer altered Bid expression can contribute to resistance against apoptotic stimuli allowing cancer cells to survive and proliferate unchecked. In neurodegenerative diseases such as Alzheimer's dysregulation of Bid and its interactions with other apoptotic proteins can lead to excessive neuronal cell death. These processes involve interactions with proteins like Bcl-2 which often act as antagonists to Bid's pro-apoptotic functions impacting disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Induces caspases and apoptosis. Counters the protective effect of BCL2.. BH3-interacting domain death agonist p15. Induces caspase activation and apoptosis (PubMed : 9727491, PubMed : 9727492). Translocates to mitochondria following cleavage and disrupts the outer mitochondrial membrane leading to the release of cytochrome c (PubMed : 9727491, PubMed : 9727492, PubMed : 9873064).

See full target information Bid

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 14:4478-4492 PubMed39525569

2024

Charge-guided masking of a membrane-destabilizing peptide enables efficient endosomal escape for targeted intracellular delivery of proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhao,Haolin Jiang,Hang Chen,Jiazhen Yu,Luyao Wang,Wen Zhou,Juanjuan Du

Antioxidants (Basel, Switzerland) 13: PubMed39061948

2024

Antioxidant and Anti-Apoptotic Neuroprotective Effects of Cinnamon in Imiquimod-Induced Lupus.

Applications

Unspecified application

Species

Unspecified reactive species

Georges Maalouly,Christine-Marie-Anne Martin,Yara Baz,Youakim Saliba,Anna-Maria Baramili,Nassim Fares

Journal of translational medicine 21:817 PubMed37974228

2023

HOXD9/miR-451a/PSMB8 axis is implicated in the regulation of cell proliferation and metastasis via PI3K/AKT signaling pathway in human anaplastic thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zhong,Fan Yu,Ling Yang,Yu Wang,Lin Liu,Chengyou Jia,Haidong Cai,Jianshe Yang,Shiyang Sheng,Zhongwei Lv,Li Weng,Bo Wu,Xiaoping Zhang

International journal of molecular sciences 24: PubMed36835418

2023

Investigation of Molecular Mechanisms Involved in Sensitivity to the Anti-Cancer Activity of Costunolide in Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jeong Choi,Youn Kyung Choi,Seong-Gyu Ko,Chunhoo Cheon,Tai Young Kim

Cell death & disease 13:602 PubMed35831279

2022

Targeting sphingosine kinase 1/2 by a novel dual inhibitor SKI-349 suppresses non-small cell lung cancer cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhang Xue,Kanqiu Jiang,Li Ou,Mingjing Shen,Yi Yang,Jingjing Lu,Weihua Xu

Disease markers 2022:3556372 PubMed35069930

2022

Apelin Alleviates Meniscus Endothelial Cell Apoptosis in Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Dinggui Lu,Jihua Wei,Jian Chen,Jingjie Zhao,Jiajia Wang,Yuanxun Gong,Liuzhi Wei,Qiuju Wei,Huadeng Ban,Yueyong Li,Zechen Wang,Changtai Luo,Haidong Zhou,Jiajia Shen,Qiujiao Liao,Siyuan He,Weiyang Zhang,Qunqiang Luo,Kegong Xie,Jian Song,Lingzhang Meng

Nature communications 11:5421 PubMed33110072

2020

Genetically engineered magnetic nanocages for cancer magneto-catalytic theranostics.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhang,Xiaoyong Wang,Chengchao Chu,Zijian Zhou,Biaoqi Chen,Xin Pang,Gan Lin,Huirong Lin,Yuxin Guo,En Ren,Peng Lv,Yesi Shi,Qingbing Zheng,Xiaohui Yan,Xiaoyuan Chen,Gang Liu

International journal of molecular medicine 46:1039-1050 PubMed32705266

2020

Liriopesides B induces apoptosis and cell cycle arrest in human non‑small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxu Sheng,Wang Lv,Linhai Zhu,Luming Wang,Zhitian Wang,Jia Han,Jian Hu

Journal of Zhejiang University. Science. B 21:234-245 PubMed32133800

2020

Amyloid precursor protein regulates 5-fluorouracil resistance in human hepatocellular carcinoma cells by inhibiting the mitochondrial apoptotic pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Long Wu,Ying Chen,Wen-Cui Kong,Zhong-Quan Zhao

OncoTargets and therapy 12:6119-6131 PubMed31496721

2019

Antitumor effect of kurarinone and underlying mechanism in small cell lung carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Wen Chung,Chi-Chien Lin,Shih-Chao Lin,Hong-Lin Chan,Ching-Chieh Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Bid Cleavage Site antibody

Immunocytochemistry/ Immunofluorescence - Anti-Bid Cleavage Site antibody (AB10640)

Western blot - Anti-Bid Cleavage Site antibody (AB10640)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

Product promise