Anti-BID (phospho S78) antibody

• IP

Supplier Data

Immunoprecipitation - Anti-BID (phospho S78) antibody (AB264131)

Whole cell lysate (1 mg for IP, 20% of IP loaded) from NIH/3T3 cells treated with 100 μM etoposide for 3 hours (+) or mock treated (-) cells. ab264131 was used for WB at 1 μg/ml. An alternative Rabbit anti-BID antibody was used for IP for BID and phospho-BID.

All lanes:

Immunoprecipitation - Anti-BID (phospho S78) antibody (ab264131)

Predicted band size: 22 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-BID (phospho S78) antibody (AB264131)

4-20% SDS-PAGE.

All lanes:

Western blot - Anti-BID (phospho S78) antibody (ab264131) at 1 µg/mL

Lane 1:

NIH/3T3 whole cell lysate untreated at 50 µg

Lane 2:

NIH/3T3 whole cell lysate treated with Etoposide (100 μM , 3 hrs) at 50 µg

Lane 3:

NIH/3T3 whole cell lysate untreated at 80 µg

Lane 4:

NIH/3T3 whole cell lysate treated with Etoposide (100 μM , 3 hrs) at 80 µg

Predicted band size: 22 kDa

false

Exposure time: 3min