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AB154236

Anti-Bif-1 antibody

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(1 Publication)

Rabbit Polyclonal Bif-1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SH3GLB1 aa 300-400.

View Alternative Names

KIAA0491, CGI-61, SH3GLB1, Endophilin-B1, Bax-interacting factor 1, SH3 domain-containing GRB2-like protein B1, Bif-1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Bif-1 antibody (AB154236)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Bif-1 antibody (AB154236)

Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling Bif-1 with ab154236 at 1/200 dilution. Lower panel merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bif-1 antibody (AB154236)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bif-1 antibody (AB154236)

Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling Bif-1 with ab154236 at 1/500 dilution.

Western blot - Anti-Bif-1 antibody (AB154236)
  • WB

Unknown

Western blot - Anti-Bif-1 antibody (AB154236)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Bif-1 antibody (ab154236) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 40 kDa,64 kDa

false

Western blot - Anti-Bif-1 antibody (AB154236)
  • WB

Unknown

Western blot - Anti-Bif-1 antibody (AB154236)

10% SDS PAGE

All lanes:

Western blot - Anti-Bif-1 antibody (ab154236) at 1/1000 dilution

Lane 1:

NIH 3T3 whole cell lysate at 30 µg

Lane 2:

JC whole cell lysate at 30 µg

Lane 3:

BCL1 whole cell lysate at 30 µg

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human SH3GLB1 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

Q9Y371

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bif-1 also known as BAX-interacting factor-1 is a protein involved in the regulation of apoptosis and autophagy. It has an approximate mass of 23 kDa and is expressed in various tissues including the brain and immune cells. The protein is an important player in mitochondrial dynamics participating in membrane curvature required for mitochondrial fission.
Biological function summary

This protein participates in mitochondrial fragmentation and membrane remodeling. Bif-1 is a component of the endophilin B1 complex which is involved in the regulation of apoptosis. It interacts directly with BAX a pro-apoptotic protein and enhances BAX translocation to mitochondria thereby facilitating the release of cytochrome c and subsequent cell death processes. It also influences autophagic processes by binding to Beclin1.

Pathways

Bif-1 functions as an important link between the apoptosis and autophagy pathways. It facilitates the intrinsic apoptotic signaling pathway where BAX and Beclin1 play significant roles. In these pathways the protein controls cellular homeostasis and stress responses linking to other key regulators such as Bak and p53 which are involved in tumor suppression and DNA damage responses.

Bif-1 has connections to neurodegenerative diseases and cancer. Its dysregulation is associated with impaired autophagy and apoptosis contributing to the pathogenesis of Alzheimer's disease. In cancers altered expression of Bif-1 affects tumor growth and resistance to cell death interacting with proteins such as p53 and BAX which are pivotal in controlling cellular proliferation and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be required for normal outer mitochondrial membrane dynamics (PubMed : 15452144). Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed : 11604418). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed : 16227588). Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed : 17891140). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed : 21068542). Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20643123). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability (By similarity).
See full target information SH3GLB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:960 PubMed28424461

2017

A lack of coordination between sister-chromatids segregation and cytokinesis in the oocytes of B6.Y (XY) sex-reversed female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Qiao Zhu,Seang Lin Tan,Teruko Taketo
View all publications
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Product promise

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