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AB180135

Anti-Bif-1 antibody [EPR11857]

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(1 Publication)

Rabbit Recombinant Monoclonal Bif-1 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

KIAA0491, CGI-61, SH3GLB1, Endophilin-B1, Bax-interacting factor 1, SH3 domain-containing GRB2-like protein B1, Bif-1

1 Images
Western blot - Anti-Bif-1 antibody [EPR11857] (AB180135)
  • WB

Supplier Data

Western blot - Anti-Bif-1 antibody [EPR11857] (AB180135)

All lanes:

Western blot - Anti-Bif-1 antibody [EPR11857] (ab180135) at 1/1000 dilution

Lane 1:

Saos-2 cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

C2C12 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 40 kDa

false

  • Carrier free

    Anti-Bif-1 antibody [EPR11857] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11857

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>The immunogen used for this product shares 81% homology with Endophilin-B2&#46; Cross-reactivity with this protein has not been confirmed experimentally.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bif-1 also known as BAX-interacting factor-1 is a protein involved in the regulation of apoptosis and autophagy. It has an approximate mass of 23 kDa and is expressed in various tissues including the brain and immune cells. The protein is an important player in mitochondrial dynamics participating in membrane curvature required for mitochondrial fission.
Biological function summary

This protein participates in mitochondrial fragmentation and membrane remodeling. Bif-1 is a component of the endophilin B1 complex which is involved in the regulation of apoptosis. It interacts directly with BAX a pro-apoptotic protein and enhances BAX translocation to mitochondria thereby facilitating the release of cytochrome c and subsequent cell death processes. It also influences autophagic processes by binding to Beclin1.

Pathways

Bif-1 functions as an important link between the apoptosis and autophagy pathways. It facilitates the intrinsic apoptotic signaling pathway where BAX and Beclin1 play significant roles. In these pathways the protein controls cellular homeostasis and stress responses linking to other key regulators such as Bak and p53 which are involved in tumor suppression and DNA damage responses.

Bif-1 has connections to neurodegenerative diseases and cancer. Its dysregulation is associated with impaired autophagy and apoptosis contributing to the pathogenesis of Alzheimer's disease. In cancers altered expression of Bif-1 affects tumor growth and resistance to cell death interacting with proteins such as p53 and BAX which are pivotal in controlling cellular proliferation and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be required for normal outer mitochondrial membrane dynamics (PubMed : 15452144). Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed : 11604418). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed : 16227588). Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed : 17891140). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed : 21068542). Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20643123). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability (By similarity).
See full target information SH3GLB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 42:13 PubMed36627634

2023

Parvimonas micra activates the Ras/ERK/c-Fos pathway by upregulating miR-218-5p to promote colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiao Chang,Ziran Huang,Fengyi Hou,Yuejiao Liu,Likun Wang,Zhen Wang,Yifan Sun,Zhiyuan Pan,Yafang Tan,Lei Ding,Hong Gao,Ruifu Yang,Yujing Bi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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