Anti-Big tau antibody [EPR26831-57]

• WB

Lab

Western blot - Anti-Big tau antibody [EPR26831-57] (AB326361)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver, kidney

The identity of the bands lower than 115 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Big tau antibody [EPR26831-57] (ab326361) at 1/1000 dilution

Lane 1:

Mouse spinal cord tissue lysate at 20 µg

Lane 2:

Mouse cerebellum tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Mouse kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 115 kDa,36 kDa

false

Exposure time: 37s

• I-ELISA

Lab

Indirect ELISA - Anti-Big tau antibody [EPR26831-57] (AB326361)

Indirect ELISA analysis of ab326361 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human Big tau protein;Human tau 2N4R protein.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with Human tau 2N4R protein by ELISA.

• WB

Lab

Western blot - Anti-Big tau antibody [EPR26831-57] (AB326361)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human tau 0N3R, 1N3R, 2N3R, 0N4R, 1N4R or 2N4R by western blot.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) 1 : 100000 (15Kda);Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(115KDa) .

All lanes:

Western blot - Anti-Big tau antibody [EPR26831-57] (ab326361) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® fresh whole cell lysate at 20 µg

Lane 2:

293T cells transfected with human Big tau expression vector containing a myc-His-tag® fresh whole cell lysate at 20 µg

Lane 3:

293T cells transfected with human 0N3R expression vector containing a myc-His-tag® fresh whole cell lysate at 20 µg

Lane 4:

293T cells transfected with human 1N3R expression vector containing a myc-His-tag® fresh whole cell lysate at 20 µg

Lane 5:

293T cells transfected with human 2N3R expression vector containing a myc-His-tag® fresh whole cell lysate at 20 µg

Lane 6:

293T cells transfected with human 0N4R expression vector containing a myc-His-tag® fresh whole cell lysate at 20 µg

Lane 7:

293T cells transfected with human 1N4R expression vector containing a myc-His-tag® fresh whole cell lysate at 20 µg

Lane 8:

293T cells transfected with human 2N4R expression vector containing a myc-His-tag® fresh whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 115 kDa,15 kDa

false

Exposure time: 1s