AB58562

Anti-Biglycan antibody

Name: Anti-Biglycan antibody (ab58562) | Abcam
Brand: Abcam Limited
SKU: AB58562
Rating: 3 (5 reviews)

(5 Reviews)

(23 Publications)

Goat Polyclonal Biglycan antibody. Suitable for ICC and reacts with Mouse, Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human BGN aa 350 to C-terminus.

View Alternative Names

SLRR1A, BGN, Biglycan, Bone/cartilage proteoglycan I, PG-S1

2 Images

Supplier Data

Immunocytochemistry - Anti-Biglycan antibody (AB58562)

Immunocytochemistry analysis of paraformaldehyde fixed, 0.15% Triton X 100 permeabilized HepG2 cells staining biglycan with ab58562 at 10μg/ml, followed by Alexa Fluor 488 secondary antibody at 2μg/ml (green). Nuclei counterstained with DAPI (blue).

Negative control : Unimmunized goat IgG (10μg/ml) followed by Alexa Fluor 488 secondary antibody (2μg/ml).

Supplier Data

Immunocytochemistry - Anti-Biglycan antibody (AB58562)

Immunocytochemistry analysis of paraformaldehyde fixed, 0.15% Triton X 100 permeabilized NIH3T3 cells staining biglycan with ab58562 at 10μg/ml, followed by Alexa Fluor 488 secondary antibody at 2μg/ml (green). Nuclei counterstained with DAPI (blue).

Negative control : Unimmunized goat IgG (10μg/ml) followed by Alexa Fluor 488 secondary antibody (2μg/ml).

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC

applications

Immunogen

Synthetic Peptide within Human BGN aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P21810

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Biglycan also known as BGN is a small leucine-rich proteoglycan with a molecular weight of roughly 38 kDa. Researchers frequently observe Biglycan in tissues such as bone cartilage and vasculature. The protein interacts with several other molecules in the extracellular matrix (ECM) including collagen and elastin to maintain the structural integrity of tissues. Through these interactions Biglycan plays an important role in supporting cell attachment and matrix organization.

Biological function summary

The protein Biglycan influences cellular processes such as growth and differentiation. It is part of a complex network within the ECM that modulates cell signaling. Biglycan interacts with growth factors like transforming growth factor-beta (TGF-beta) and binds to Toll-like receptors (TLRs) affecting immune responses. These capabilities reveal Biglycan’s involvement in tissue development and repair.

Pathways

Extracellular matrix modulation shows a significant role for Biglycan. It is involved in the TGF-beta signaling pathway and the inflammatory pathway mediated by TLRs. Through these pathways Biglycan interacts with proteins such as decorin and fibromodulin contributing to the regulation of collagen fibrillogenesis and inflammatory responses. Its role in these pathways highlights Biglycan’s influence in cellular communication and matrix regulation.

Biglycan has associations with osteoarthritis and muscular dystrophy. In osteoarthritis researchers observe altered expression levels of Biglycan which affects cartilage integrity. In muscular dystrophy the protein’s connection to dystrophin an important structural protein is noted. These associations illustrate Biglycan's impact on disease progression and its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in collagen fiber assembly.

See full target information BGN

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Liver international : official journal of the International Association for the Study of the Liver 43:500-512 PubMed36371672

2022

Biglycan promotes hepatic fibrosis through activating heat shock protein 47.

Applications

Unspecified application

Species

Unspecified reactive species

Mengli Yu,Xinjue He,Xin Song,Jianguo Gao,Jiaqi Pan,Tianyu Zhou,Qinqiu Wang,Wei Zhu,Huijian Ma,Hang Zeng,Chengfu Xu,Chaohui Yu

Acta biochimica et biophysica Sinica 54:243-251 PubMed35130618

2022

Aberrant expression of the extracellular matrix component Biglycan regulated by Hedgehog signalling promotes colorectal cancer cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Shaopeng Zeng,Feifei Zhou,Yiqing Wang,Zhenyu Zhai,Linlin Xu,Hailong Wang,Xinping Chen,Shiwen Luo,Minzhang Cheng

Journal of cellular and molecular medicine 25:4195-4203 PubMed33745198

2021

Bmi1 regulate tooth and mandible development by inhibiting p16 signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Yin,Nan Zhou,Hui Zhang,Xiuliang Dai,Xianhui Lv,Ning Chen,Dengshun Miao,Qingang Hu

Aging 11:7257-7273 PubMed31509519

2019

Genomic deletion of TLR2 induces aggravated white matter damage and deteriorated neurobehavioral functions in mouse models of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zhou,Xiaoyu Sun,Yuting Hu,Jiaxing Song,Shuyu Dong,Delian Kong,Yuqiao Wang,Xiaodong Hua,Jingjing Han,Yan Zhou,Guoliang Jin,Xinxin Yang,Hongjuan Shi,Zuohui Zhang,Fang Hua

In vitro cellular & developmental biology. Animal 55:211-219 PubMed30756235

2019

Effect of attenuation of fibroblast growth factor receptor 2b signaling on odontoblast differentiation and dentin formation.

Applications

Unspecified application

Species

Unspecified reactive species

Miyuki Yokoi,Koh-Ichi Kuremoto,Shinsuke Okada,Miwa Sasaki,Kazuhiro Tsuga

Journal of proteomics 191:212-227 PubMed29408692

2018

Proteomic analysis of the sarcolemma-enriched fraction from dystrophic mdx-4cv skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Murphy,Margit Zweyer,Michael Henry,Paula Meleady,Rustam R Mundegar,Dieter Swandulla,Kay Ohlendieck

Journal of muscle research and cell motility 38:251-268 PubMed28803268

2017

Proteomic profiling of the dystrophin complex and membrane fraction from dystrophic mdx muscle reveals decreases in the cytolinker desmoglein and increases in the extracellular matrix stabilizers biglycan and fibronectin.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Murphy,Heinrich Brinkmeier,Mirjam Krautwald,Michael Henry,Paula Meleady,Kay Ohlendieck

European cells & materials 32:228-240 PubMed27763655

2016

Periodontal ligament cells as alternative source for cell-based therapy of tendon injuries: in vivo study of full-size Achilles tendon defect in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

C-F Hsieh,P Alberton,E Loffredo-Verde,E Volkmer,M Pietschmann,P E Müller,M Schieker,D Docheva

PloS one 10:e0128163 PubMed26197235

2015

Foam Cell Formation In Vivo Converts Macrophages to a Pro-Fibrotic Phenotype.

Applications

Unspecified application

Species

Mouse

Anita C Thomas,Wouter J Eijgelaar,Mat J A P Daemen,Andrew C Newby

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 63:8-21 PubMed25362141

2014

Study of the development of the mouse thoracic aorta three-dimensional macromolecular structure using two-photon microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Leah M Zadrozny,Edward B Neufeld,Bertrand M Lucotte,Patricia S Connelly,Zu-Xi Yu,Lam Dao,Li-Yueh Hsu,Robert S Balaban

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Biglycan antibody

Immunocytochemistry - Anti-Biglycan antibody (AB58562)

Immunocytochemistry - Anti-Biglycan antibody (AB58562)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

Product promise