Rabbit Recombinant Monoclonal BIII antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-BIII antibody [EPR25384-127] (AB288155), expandable thumbnail](https://content.abcam.com/products/images/biii-antibody-epr25384-127-ab288155--western-blot-img173951.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BIII antibody [EPR25384-127] (AB288155)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIII antibody [EPR25384-127] (AB288155), expandable thumbnail](https://content.abcam.com/products/images/biii-antibody-epr25384-127-ab288155--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165992.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIII antibody [EPR25384-127] (AB288155)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIII antibody [EPR25384-127] (AB288155), expandable thumbnail](https://content.abcam.com/products/images/biii-antibody-epr25384-127-ab288155--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166832.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIII antibody [EPR25384-127] (AB288155)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | IHC-Fr | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Unsuitable with Rat and Human. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Unsuitable with Rat and Human. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Unsuitable with Rat and Human. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. This alpha-1B subunit gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group. They are involved in pain signaling (PubMed:25296916). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons. Mediates Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals (By similarity). Isoform Alpha-1B-1. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. This alpha-1B subunit gives rise to N-type calcium currents.
Alternative names
CACH5, CACNL1A5, CACNA1B, Voltage-dependent N-type calcium channel subunit alpha-1B, Brain calcium channel III, Voltage-gated calcium channel subunit alpha Cav2.2, BIII
Recommended products
Rabbit Recombinant Monoclonal BIII antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR25384-127
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-BIII antibody [EPR25384-127] (ab288155), expandable thumbnail](https://content.abcam.com/products/images/biii-antibody-epr25384-127-ab288155--western-blot-img173951.jpg "Western blot - Anti-BIII antibody [EPR25384-127] (ab288155)")
Western blot - Anti-BIII antibody [EPR25384-127] (ab288155)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Bis-Tris gel was used for this blot.
Samples are non-boiled as boiling may cause protein aggregates.
The lower bands (75-150kda) may represent different isoforms or proteolytic fragments according to one new study on KO mice (PMID:34353899).
Negative control: liver
Exposure time: Lanes 1-6: 37 seconds Lanes 7-8: 81 seconds
All lanes: Western blot - Anti-BIII antibody [EPR25384-127] (ab288155) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse hippocampus tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 40 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat hippocampus tissue lysate at 20 µg
Lane 6: Rat liver tissue lysate at 40 µg
Lane 7: Human cerebellum tissue lysate at 20 µg
Lane 8: Human liver tissue lysate at 40 µg
SecondaryLanes 1 - 8: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution
Lanes 1 - 8: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 262 kDa
Observed band size: 262 kDa, 75-150 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIII antibody [EPR25384-127] (ab288155), expandable thumbnail](https://content.abcam.com/products/images/biii-antibody-epr25384-127-ab288155--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165992.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIII antibody [EPR25384-127] (ab288155)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIII antibody [EPR25384-127] (ab288155)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling BIII with ab288155 at 1/100 (4.84 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining in mouse cerebrum.The section was incubated with ab288155 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIII antibody [EPR25384-127] (ab288155), expandable thumbnail](https://content.abcam.com/products/images/biii-antibody-epr25384-127-ab288155--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166832.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIII antibody [EPR25384-127] (ab288155)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIII antibody [EPR25384-127] (ab288155)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling BIII with ab288155 at 1/100 (4.84 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse liver. The section was incubated with ab288155 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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- Visit the general protocols
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