Anti-BIII antibody [EPR25384-127] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIII antibody [EPR25384-127] - BSA and Azide free (AB288162)

This data was developed using ab288155, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling BIII with ab288155 at 1/100 (4.84 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse liver. The section was incubated with ab288155 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIII antibody [EPR25384-127] - BSA and Azide free (AB288162)

This data was developed using ab288155, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling BIII with ab288155 at 1/100 (4.84 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) . Cytoplasmic staining in mouse cerebrum.The section was incubated with ab288155 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Lab

Western blot - Anti-BIII antibody [EPR25384-127] - BSA and Azide free (AB288162)

This data was developed using ab288155, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Bis-Tris gel was used for this blot.

Samples are non-boiled as boiling may cause protein aggregates.

The lower bands (75-150kda) may represent different isoforms or proteolytic fragments according to one new study on KO mice (PMID : 34353899).

Negative control : liver

Exposure time : Lane 1-6 : 37 seconds Lane 7-8 : 81 seconds

All lanes:

Western blot - Anti-BIII antibody [EPR25384-127] (<a href='/en-us/products/primary-antibodies/biii-antibody-epr25384-127-ab288155'>ab288155</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse hippocampus tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 40 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat hippocampus tissue lysate at 20 µg

Lane 6:

Rat liver tissue lysate at 40 µg

Lane 7:

Human cerebellum tissue lysate at 20 µg

Lane 8:

Human liver tissue lysate at 40 µg

Secondary

Lanes 1 - 8:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

Lanes 1 - 8:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 262 kDa

Observed band size: 262 kDa,75-150 kDa

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