Anti-Bik antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Bik antibody (AB52182)

ICC/IF image of ab52182 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52182, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bik antibody (AB52182)

ab52182 at 1/50 dilution staining Human breast carcinoma tissue, with and without blocking peptide; paraffin embedded.

• ICC/IF

AbReview22856****

Immunocytochemistry/ Immunofluorescence - Anti-Bik antibody (AB52182)

ab52182 staining Bik in human Jurkat cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X and then blocked using 3% serum for 3 hours at 23°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 647 (pink) used at a 1/1000 dilution.

Image courtesy of an anonymous Abreview.

• WB

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Western blot - Anti-Bik antibody (AB52182)

All lanes:

Western blot - Anti-Bik antibody (ab52182) at 1/500 dilution

Lane 1:

extracts from A549 cells, treatedwith DMSO (0.1%, 10mins)

Lane 2:

extracts from A549 cells, treatedwith DMSO (0.1%, 10mins) with blocking peptide

Predicted band size: 18 kDa

Observed band size: 30 kDa

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