Rabbit Polyclonal Bim antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human BCL2L11 aa 1-50.
Images
Publications
- Journal of Cancer 12:7277-72862021CPNE3 regulates the cell proliferation and apoptosis in human Glioblastoma via the activation of PI3K/AKT signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDainan Zhang,Xiaoyin Wang,Xi Wang,Zemin Wang,Shunchang Ma,Chuanbao Zhang,Shaomin Li,Wang JiaPubMed 35003348
- Theranostics 11:996-10152021The 14-3-3σ protein promotes HCC anoikis resistance by inhibiting EGFR degradation and thereby activating the EGFR-dependent ERK1/2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Song,Yachong Liu,Furong Liu,Lu Zhang,Ganxun Li,Chaoyi Yuan,Chengpeng Yu,Xun Lu,Qiumeng Liu,Xiaoping Chen,Huifang Liang,Zeyang Ding,Bixiang ZhangPubMed 33391517
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human BCL2L11 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O43521
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.
Alternative names
Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BIM, BCL2L11
Recommended products
Rabbit Polyclonal Bim antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human BCL2L11 aa 1-50.
Alternative names
Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BIM, BCL2L11
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human BCL2L11 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O43521
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
Bim also known as Bcl-2-interacting mediator of cell death is an important pro-apoptotic protein within the Bcl-2 family. It has a molecular weight of approximately 23 kDa. Bim is expressed in various tissues including the immune system nervous system and lymphoid tissues. It exists in multiple isoforms such as BimEL BimL and BimS due to alternative splicing. Bim's interaction with cellular membranes allows it to regulate apoptotic processes through mitochondrial pathways effectively.
- Biological function summary
Bim regulates apoptosis by binding to pro-survival proteins like Bcl-2 and Bcl-xL releasing pro-apoptotic factors from mitochondria and activating caspases. Bim acts as part of the apoptosome complex and influences cell death regulation significantly. By promoting cytochrome c release from mitochondria Bim initiates a cascade of events leading to cell apoptosis. This regulation is vital in maintaining the balance between cell survival and death necessary for normal development and tissue homeostasis.
- Pathways
Bim plays a critical role in the intrinsic apoptotic pathway. This pathway involves mitochondrial outer membrane permeabilization where Bim interacts with several Bcl-2 family proteins such as Bax and Bak to induce apoptosis. The modulation of Bim expression and activity is influenced by growth factor signaling pathways such as the PI3K-Akt pathway which affects Bim phosphorylation leading to its inactivation and subsequent degradation. Therefore Bim acts as an important node linking survival signals and apoptotic machinery.
- Associated diseases and disorders
Bim dysregulation has been implicated in conditions like cancer and autoimmune diseases. In certain cancers reduced Bim expression can result in unchecked cell proliferation and resistance to apoptosis. Conversely in autoimmune disorders overactivity of Bim may lead to excessive immune cell apoptosis contributing to disease pathogenesis. Bim's interactions with proteins like Bcl-2 and Bcl-xL are significant in cancer therapy as targeting these interactions can help overcome resistance to apoptosis in cancer cells improving treatment outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-Bim antibody (ab7888)
All lanes: Western blot - Anti-Bim antibody (ab7888) at 0.5 µg/mL
Lane 1: Human thymus tissue lysate at 15 µg
Lane 2: Human colon tissue lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 22 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bim antibody (ab7888)
ab7888 at 20μg/ml staining Bim in human cancer cells by IHC-P
Western blot - Anti-Bim antibody (ab7888)
All lanes: Western blot - Anti-Bim antibody (ab7888) at 0.5 µg/mL
All lanes: Rat Myeloma Cell lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 22 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody (ab7888)
Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed human K562 cells labeling Bim with ab7888 at 20 μg/mL. Goat anti-rabbit IgG secondary antibody at 1/500 dilution was used as the secondry antibody.
Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody (ab7888)
ICC/IF image of ab7888 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7888, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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