Anti-Bim antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bim antibody (AB7888)

ab7888 at 20μg/ml staining Bim in human cancer cells by IHC-P

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody (AB7888)

Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed human K562 cells labeling Bim with ab7888 at 20 μg/mL. Goat anti-rabbit IgG secondary antibody at 1/500 dilution was used as the secondry antibody.

• WB

Supplier Data

Western blot - Anti-Bim antibody (AB7888)

All lanes:

Western blot - Anti-Bim antibody (ab7888) at 0.5 µg/mL

All lanes:

Rat Myeloma Cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 22 kDa

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• WB

Supplier Data

Western blot - Anti-Bim antibody (AB7888)

All lanes:

Western blot - Anti-Bim antibody (ab7888) at 0.5 µg/mL

Lane 1:

Human thymus tissue lysate at 15 µg

Lane 2:

Human colon tissue lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 22 kDa

false

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody (AB7888)

ICC/IF image of ab7888 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7888, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.