JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB17935

Anti-Bim (phospho S65) antibody

Be the first to review this product! Submit a review

|

(7 Publications)

Rabbit Polyclonal Bim phospho S65 antibody. Suitable for WB and reacts with Synthetic peptide - Rat samples. Cited in 7 publications.

View Alternative Names

Bim, Bod, Bcl2l11, Bcl-2-like protein 11, Bcl2-L-11, Bcl-2-related ovarian death protein, Bcl2-interacting mediator of cell death

1 Images
Western blot - Anti-Bim (phospho S65) antibody (AB17935)
  • WB

Unknown

Western blot - Anti-Bim (phospho S65) antibody (AB17935)

Lysates prepared from Hek293T cells transfected with rat WT Bim alone (1, 4), cotransfected with rat WT Bim and activated MEKK1 (2, 5) or with
mutant S65A Bim and MEKK1 (3, 6), were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with 3% Milk-TBST buffer for one hour at room temperature, and incubated with Bim Pan antibody (1, 2, 3) or ab17935 (4, 5, 6) for two hours at room temperature in 3% Milk-TBST buffer. After washing, membranes were incubated with a goat F(ab')2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.

The data show that the signal is detected only in lysates co-expressing rat WT Bim and active MEKK1. This signal is abolished in cells expressing mutant rat Bim S65A and MEKK1, verifying that the signal is site-phosphospecific.

Lysates prepared from Hek293T cells transfected with rat WT Bim alone (1, 4), cotransfected with rat WT Bim and activated MEKK1 (2

All lanes:

Western blot - Anti-Bim (phospho S65) antibody (ab17935)

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB7888

Anti-Bim antibody

Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody (AB7888)

  • 0
  • 0
Primary Antibodies

AB131477

Anti-Bim (phospho S69) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Bim (phospho S69) antibody (AB131477)

  • 0
  • 0
Primary Antibodies

AB32158

Anti-Bim antibody [Y36]

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-Bim antibody [Y36] (AB32158)

  • 4
  • 6
Primary Antibodies

AB170589

Anti-Bim antibody [Y36] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Bim antibody [Y36] - BSA and Azide free (AB170589)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
Primary Antibodies

AB70122

Anti-ARNT2 antibody

Western blot - Anti-ARNT2 antibody (AB70122)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

  • 5
  • 13
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Synthetic peptide - Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a Bim-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence).
Storage buffer
pH: 7.3 Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bim also known as Bcl-2-interacting mediator of cell death is an important pro-apoptotic protein within the Bcl-2 family. It has a molecular weight of approximately 23 kDa. Bim is expressed in various tissues including the immune system nervous system and lymphoid tissues. It exists in multiple isoforms such as BimEL BimL and BimS due to alternative splicing. Bim's interaction with cellular membranes allows it to regulate apoptotic processes through mitochondrial pathways effectively.
Biological function summary

Bim regulates apoptosis by binding to pro-survival proteins like Bcl-2 and Bcl-xL releasing pro-apoptotic factors from mitochondria and activating caspases. Bim acts as part of the apoptosome complex and influences cell death regulation significantly. By promoting cytochrome c release from mitochondria Bim initiates a cascade of events leading to cell apoptosis. This regulation is vital in maintaining the balance between cell survival and death necessary for normal development and tissue homeostasis.

Pathways

Bim plays a critical role in the intrinsic apoptotic pathway. This pathway involves mitochondrial outer membrane permeabilization where Bim interacts with several Bcl-2 family proteins such as Bax and Bak to induce apoptosis. The modulation of Bim expression and activity is influenced by growth factor signaling pathways such as the PI3K-Akt pathway which affects Bim phosphorylation leading to its inactivation and subsequent degradation. Therefore Bim acts as an important node linking survival signals and apoptotic machinery.

Bim dysregulation has been implicated in conditions like cancer and autoimmune diseases. In certain cancers reduced Bim expression can result in unchecked cell proliferation and resistance to apoptosis. Conversely in autoimmune disorders overactivity of Bim may lead to excessive immune cell apoptosis contributing to disease pathogenesis. Bim's interactions with proteins like Bcl-2 and Bcl-xL are significant in cancer therapy as targeting these interactions can help overcome resistance to apoptosis in cancer cells improving treatment outcomes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Induces apoptosis and anoikis.
See full target information Bcl2l11 phospho S65
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1491546 PubMed39584135

2024

Huyang Yangkun formula regulates the mitochondria pathway of ovarian granulosa cell apoptosis through FTO/m6A-P53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Lingdi Wang,Jian Liu,Guangning Nie,Hongyan Yang

Evidence-based complementary and alternative medicine : eCAM 2021:6614302 PubMed34035823

2021

Danggui Buxue Tang Rescues Folliculogenesis and Ovarian Cell Apoptosis in Rats with Premature Ovarian Insufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Lingdi Wang,Jian Liu,Guangning Nie,Yang Li,Hongyan Yang

General physiology and biophysics 39:145-155 PubMed32329442

2020

Inhibition of HES-1 might play a protective role in endothelial cells under cholesterol stimulation via PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Shi,Ya Su,Chuanli Liu,Jinglong Tao,Hao Fu,Chengzhi Lu

Oncology reports 42:1183-1193 PubMed31233204

2019

Synergistic antitumor effect of BRMS1 and sorafenib via inhibition of the PI3K/AKT/mTOR/ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyun Jin,Yonggang Guo,Yang Liu,Zhenguo Wang,Xiaosa Yang,Shengkai Sun

PloS one 10:e0132998 PubMed26171786

2015

Involvement of the JNK/FOXO3a/Bim Pathway in Neuronal Apoptosis after Hypoxic-Ischemic Brain Damage in Neonatal Rats.

Applications

IF

Species

Rat

Deyuan Li,Xihong Li,Jinlin Wu,Jinhui Li,Li Zhang,Tao Xiong,Jun Tang,Yi Qu,Dezhi Mu

Proceedings of the National Academy of Sciences of the United States of America 110:E4894-903 PubMed24282297

2013

Tuberous sclerosis 1 (Tsc1)-dependent metabolic checkpoint controls development of dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Wang,Gonghua Huang,Hu Zeng,Kai Yang,Richard F Lamb,Hongbo Chi

Proceedings of the National Academy of Sciences of the United States of America 109:E343-52 PubMed22308391

2012

Transforming growth factor beta-activated kinase 1 (TAK1)-dependent checkpoint in the survival of dendritic cells promotes immune homeostasis and function.

Applications

WB

Species

Mouse

Yanyan Wang,Gonghua Huang,Peter Vogel,Geoffrey Neale,Boris Reizis,Hongbo Chi
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com