Rabbit Polyclonal BIN1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BIN1 aa 500 to C-terminus.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human BIN1 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00499
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2500.00000 | Notes - |
Associated Products
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Target data
Function
Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling (PubMed:24755653). Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production (PubMed:27179792). In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity). May be involved in the regulation of MYC activity and the control cell proliferation (PubMed:8782822). Has actin bundling activity and stabilizes actin filaments against depolymerization in vitro (PubMed:28893863).
Alternative names
Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, AMPHL, BIN1
Recommended products
Rabbit Polyclonal BIN1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BIN1 aa 500 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human BIN1 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00499
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody (ab264303)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling BIN1 with ab264303 at 1/1,000 dilution. Detection: DAB.
Western blot - Anti-BIN1 antibody (ab264303)
4-8% SDS-PAGE
All lanes: Western blot - Anti-BIN1 antibody (ab264303) at 1 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
SecondaryAll lanes: Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 65 kDa
Exposure time: 3min
Immunoprecipitation - Anti-BIN1 antibody (ab264303)
BIN1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264303 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab264303 at 1 μg/ml.
Lane 1: ab264303 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 mins.
All lanes: Immunoprecipitation - Anti-BIN1 antibody (ab264303)
Predicted band size: 65 kDa
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