Rabbit Recombinant Monoclonal BIN1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 6 publications.
Images
![Western blot - Anti-BIN1 antibody [EPR13463-25] (AB185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--western-blot-img1477.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BIN1 antibody [EPR13463-25] (AB185950)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (AB185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img20494.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (AB185950)")
![Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (AB185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--immunocytochemistry-immunofluorescence-img108177.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (AB185950)")
![Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (AB185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--flow-cytometry-intracellular-img57827.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (AB185950)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (AB185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118022.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (AB185950)")
Publications
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- International journal of molecular sciences 24:2022Polycystin-1 Is a Crucial Regulator of BIN1 Expression and T-Tubule Remodeling Associated with the Development of Dilated Cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMagda C Díaz-Vesga,Raúl Flores-Vergara,Jaime A Riquelme,Marcelo Llancaqueo,Gina Sánchez,Cecilia Vergara,Luis Michea,Paulina Donoso,Andrew F G Quest,Ivonne Olmedo,Zully PedrozoPubMed 36614108
- Experimental & molecular medicine 54:493-5022022Tomatidine-stimulated maturation of human embryonic stem cell-derived cardiomyocytes for modeling mitochondrial dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe Seul Kim,Jung Won Yoon,Dasol Kim,Seunghak Choi,Hyoung Kyu Kim,Jae Boum Youm,Jin Han,Soon Chul Heo,Sung-Ae Hyun,Jung-Wook Seo,Deok-Ho Kim,Jae Ho KimPubMed 35379934
- The Journal of biological chemistry 294:4477-44872019Reduction of the expression of the late-onset Alzheimer's disease (AD) risk-factor does not affect amyloid pathology in an AD mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert J Andrew et. al.PubMed 30692199
- Journal of cellular biochemistry 120:4644-46532018microRNA-211 promotes proliferation, migration, and invasion ability of oral squamous cell carcinoma cells via targeting the bridging integrator 1 protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiabao Zheng,Jiali Wang,Yunlong Jia,Tianxu Liu,Yuqing Duan,Xing Liang,Lihua LiuPubMed 30260023
- Molecular neurodegeneration 12:822017Multiscale network modeling of oligodendrocytes reveals molecular components of myelin dysregulation in Alzheimer's disease.
- Oncogene 36:6235-62432017BIN1 reverses PD-L1-mediated immune escape by inactivating the c-MYC and EGFR/MAPK signaling pathways in non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Wang,Y Jia,S Zhao,X Zhang,X Wang,X Han,Y Wang,M Ma,J Shi,L LiuPubMed 28714960
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested |
| Rat | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/90 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling (PubMed:24755653). Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production (PubMed:27179792). In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity). May be involved in the regulation of MYC activity and the control cell proliferation (PubMed:8782822). Has actin bundling activity and stabilizes actin filaments against depolymerization in vitro (PubMed:28893863).
Alternative names
AMPHL, BIN1, Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1
Recommended products
Rabbit Recombinant Monoclonal BIN1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13463-25
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--western-blot-img1477.jpg "Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)")
Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: BIN1 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: U-87MG whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - ab185950 observed at 45-65 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab185950 was shown to specifically react with BIN1 when BIN1 knockout samples were used. Wild-type and BIN1 knockout samples were subjected to SDS-PAGE. ab185950 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
Predicted band size: 65 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img20494.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--immunocytochemistry-immunofluorescence-img108177.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)")
Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)
Immunofluorescent analysis of HeLa cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: -20℃ Acetone. Counter stain: Dapi (blue).
![Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (ab185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--flow-cytometry-intracellular-img57827.jpg "Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (ab185950)")
Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
Intracellular flow cytometric analysis ofHela cells labeling BIN1 using ab185950 at 1/90 dilution (red). Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG (green).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118022.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--western-blot-img103678.jpg "Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)")
Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
Blocking buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950) at 1/1000 dilution
Lane 1: U87-MG cell lysate at 10 µg
Lane 2: Human fetal kidney lysate at 10 µg
Lane 3: Hela cell lysate at 10 µg
Lane 4: Human fetal brain lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 65 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--immunocytochemistry-immunofluorescence-img71226.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)")
Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)
Immunofluorescent analysis of U87-MG cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi (blue)
![Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--western-blot-img103678.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)")
![Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950), expandable thumbnail](https://content.abcam.com/products/images/bin1-antibody-epr13463-25-ab185950--immunocytochemistry-immunofluorescence-img71226.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)")
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