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AB185950

Anti-BIN1 antibody [EPR13463-25]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

5

(4 Reviews)

|

(6 Publications)

Rabbit Recombinant Monoclonal BIN1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 6 publications.

View Alternative Names

AMPHL, BIN1, Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (AB185950)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (AB185950)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab : Ready to use HRP Polymer for Rabbit IgG. Counter stain : Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (AB185950)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (AB185950)

Intracellular flow cytometric analysis ofHela cells labeling BIN1 using ab185950 at 1/90 dilution (red). Secondary ab : Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative : 2% paraformaldehyde. Isotype control : Rabbit monoclonal IgG (green).

Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (AB185950)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (AB185950)

Immunofluorescent analysis of U87-MG cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab : Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative : 4% paraformaldehyde. Counter stain : Dapi (blue)

Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (AB185950)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (AB185950)

Immunofluorescent analysis of HeLa cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab : Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative : -20℃ Acetone. Counter stain : Dapi (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (AB185950)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (AB185950)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab : Ready to use HRP Polymer for Rabbit IgG. Counter stain : Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-BIN1 antibody [EPR13463-25] (AB185950)
  • WB

Lab

Western blot - Anti-BIN1 antibody [EPR13463-25] (AB185950)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : BIN1 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : U-87MG whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab185950 observed at 45-65 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab185950 was shown to specifically react with BIN1 when BIN1 knockout samples were used. Wild-type and BIN1 knockout samples were subjected to SDS-PAGE. ab185950 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)

Predicted band size: 65 kDa

false

Western blot - Anti-BIN1 antibody [EPR13463-25] (AB185950)
  • WB

Supplier Data

Western blot - Anti-BIN1 antibody [EPR13463-25] (AB185950)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950) at 1/1000 dilution

Lane 1:

U87-MG cell lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

Hela cell lysate at 10 µg

Lane 4:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 65 kDa

false

  • Carrier free

    Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13463-25

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/90", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling (PubMed : 24755653). Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production (PubMed : 27179792). In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity). May be involved in the regulation of MYC activity and the control cell proliferation (PubMed : 8782822). Has actin bundling activity and stabilizes actin filaments against depolymerization in vitro (PubMed : 28893863).
See full target information BIN1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36614108

2022

Polycystin-1 Is a Crucial Regulator of BIN1 Expression and T-Tubule Remodeling Associated with the Development of Dilated Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Magda C Díaz-Vesga,Raúl Flores-Vergara,Jaime A Riquelme,Marcelo Llancaqueo,Gina Sánchez,Cecilia Vergara,Luis Michea,Paulina Donoso,Andrew F G Quest,Ivonne Olmedo,Zully Pedrozo

Experimental & molecular medicine 54:493-502 PubMed35379934

2022

Tomatidine-stimulated maturation of human embryonic stem cell-derived cardiomyocytes for modeling mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Seul Kim,Jung Won Yoon,Dasol Kim,Seunghak Choi,Hyoung Kyu Kim,Jae Boum Youm,Jin Han,Soon Chul Heo,Sung-Ae Hyun,Jung-Wook Seo,Deok-Ho Kim,Jae Ho Kim

The Journal of biological chemistry 294:4477-4487 PubMed30692199

2019

Reduction of the expression of the late-onset Alzheimer's disease (AD) risk-factor does not affect amyloid pathology in an AD mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Robert J Andrew,Pierre De Rossi,Phuong Nguyen,Haley R Kowalski,Aleksandra J Recupero,Thomas Guerbette,Sofia V Krause,Richard C Rice,Lisa Laury-Kleintop,Steven L Wagner,Gopal Thinakaran

Journal of cellular biochemistry 120:4644-4653 PubMed30260023

2018

microRNA-211 promotes proliferation, migration, and invasion ability of oral squamous cell carcinoma cells via targeting the bridging integrator 1 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Jiabao Zheng,Jiali Wang,Yunlong Jia,Tianxu Liu,Yuqing Duan,Xing Liang,Lihua Liu

Molecular neurodegeneration 12:82 PubMed29110684

2017

Multiscale network modeling of oligodendrocytes reveals molecular components of myelin dysregulation in Alzheimer's disease.

Applications

IHC-P, WB

Species

Mouse, Mouse

Andrew T McKenzie,Sarah Moyon,Minghui Wang,Igor Katsyv,Won-Min Song,Xianxiao Zhou,Eric B Dammer,Duc M Duong,Joshua Aaker,Yongzhong Zhao,Noam Beckmann,Pei Wang,Jun Zhu,James J Lah,Nicholas T Seyfried,Allan I Levey,Pavel Katsel,Vahram Haroutunian,Eric E Schadt,Brian Popko,Patrizia Casaccia,Bin Zhang

Oncogene 36:6235-6243 PubMed28714960

2017

BIN1 reverses PD-L1-mediated immune escape by inactivating the c-MYC and EGFR/MAPK signaling pathways in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

J Wang,Y Jia,S Zhao,X Zhang,X Wang,X Han,Y Wang,M Ma,J Shi,L Liu
View all publications
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Product promise

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