Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (AB226006)

Immunofluorescent analysis of HeLa cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab : Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative : -20°C Acetone. Counter stain : Dapi (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185950).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (AB226006)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab : Ready to use HRP Polymer for Rabbit IgG. Counter stain : Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185950).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (AB226006)

Immunofluorescent analysis of U87-MG cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab : Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative : 4% paraformaldehyde. Counter stain : Dapi (blue)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185950).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (AB226006)

Intracellular flow cytometric analysis of Hela cells labeling BIN1 using ab185950 at 1/90 dilution (red). Secondary ab : Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative : 2% paraformaldehyde. Isotype control : Rabbit monoclonal IgG (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185950).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (AB226006)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab : Ready to use HRP Polymer for Rabbit IgG. Counter stain : Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185950).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (AB226006)

This WB data was generated using the same anti-BIN1 antibody clone [EPR13463-25] in a different buffer formulation (cat# ab185950).

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : BIN1 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : U-87MG whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab185950 observed at 45-65 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab185950 was shown to specifically react with BIN1 when BIN1 knockout samples were used. Wild-type and BIN1 knockout samples were subjected to SDS-PAGE. ab185950 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-BIN1 antibody [EPR13463-25] (<a href='/en-us/products/primary-antibodies/bin1-antibody-epr13463-25-ab185950'>ab185950</a>)

Predicted band size: 65 kDa

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