AB106261

Biotin Anti-6X His tag® antibody [33D10.D2]

Name: Anti-6X His tag® Biotin antibody [33D10.D2] (ab106261) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB106261

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(1 Publication)

Mouse Monoclonal 6X His tag® antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Tag samples. Cited in 1 publication.

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

2 Images

ELISA - Biotin Anti-6X His tag® antibody [33D10.D2] (AB106261)

ELISA

Supplier Data

ELISA - Biotin Anti-6X His tag® antibody [33D10.D2] (AB106261)

ELISA analysis of ab106261 Mouse monoclonal [33D10.D2] to 6X His tag® (Biotin).
Antigen : HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins.
Coating amount : 0.15 µg per welll.
Primary antibody : ab106261 at 100 µg/mL.
Dilution series : 2-fold.
Mid-point concentration : 200 ng/mL.
Secondary antibody : Peroxidase conjugated mouse secondary antibody at 1/10000.
Substrate : TMB ELISA Substrate.

Supplier Data

Western blot - Biotin Anti-6X His tag® antibody [33D10.D2] (AB106261)

Block : 5% non-fat powdered milk for 1 hour at room temperature.

All lanes:

Western blot - Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261) at 1/5000 dilution

Lane 1:

Purified histidine-tagged recombinant protein at 0.1 µg

Lane 2:

E. coli cell lysate containing histidine-tagged expression construct at 0.2 µg

Lane 3:

Purified GST-tagged recombinant protein at 0.1 µg

Lane 4:

Purified FLAG-tagged recombinant protein at 0.1 µg

Secondary

All lanes:

Peroxidase mouse secondary antibody for 30 minutes at room temperature at 1/20000 dilution

Predicted band size: 1 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

33D10.D2

Isotype

IgG1

Conjugation

Biotin

Excitation/Emission

Carrier free

Applications

ELISA, WB

applications

Specificity

This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/200000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

10-20 BAC molecules per Mouse Monoclonal Antibody

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Anti-6X HIS Epitope Tag was purified from concentrated tissue culture supernate by Protein A chromatography and is directed against the 6X His motif and is useful in determining its presence in various assays.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC obesity 3:35 PubMed27462403

2016

Adipocyte nuclei captured from VAT and SAT.

Applications

Unspecified application

Species

Unspecified reactive species

Suresh Ambati,Ping Yu,Elizabeth C McKinney,Muthugapatti K Kandasamy,Diane Hartzell,Clifton A Baile,Richard B Meagher

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-6X His tag® antibody [33D10.D2]

ELISA - Biotin Anti-6X His tag® antibody [33D10.D2] (AB106261)

Western blot - Biotin Anti-6X His tag® antibody [33D10.D2] (AB106261)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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