AB27025

Biotin Anti-6X His tag® antibody

Name: Biotin Anti-6X His tag® antibody (ab27025) | Abcam
Brand: Abcam Limited
SKU: AB27025
Rating: 5 (3 reviews)

(3 Reviews)

(8 Publications)

Anti-6X His tag® antibody - Biotin conjugated (ab27025) is a rabbit polyclonal antibody detecting 6X His tag® in Western Blot, Flow Cytometry, ELISA.

- Trusted since 2005

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

1 Images

ELISA - Biotin Anti-6X His tag® antibody (AB27025)

ELISA

Supplier Data

ELISA - Biotin Anti-6X His tag® antibody (AB27025)

Antigen concentration : 1000 ng/well. Secondary : Avidin-HRP 100 ng/mL. Developed with TMB (10 minutes). Microplate reader set at 450nm.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Applications

ELISA, WB, Flow Cyt

applications

Specificity

ab27025 reacts to recombinant proteins containing the 6X His tag®.

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Reactivity data

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Product details

What is this antibody validated in?
Biotin Anti-6X His tag® antibody (ab27025) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), ELISA. Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

HIS-TAG® is a trademark of EMD Biosciences, Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was purified using the peptide immobilized on a solid support.

Storage buffer

pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical microbiology 59: PubMed33127841

2021

Highly Sensitive and Specific Multiplex Antibody Assays To Quantify Immunoglobulins M, A, and G against SARS-CoV-2 Antigens.

Applications

Unspecified application

Species

Unspecified reactive species

Carlota Dobaño,Marta Vidal,Rebeca Santano,Alfons Jiménez,Jordi Chi,Diana Barrios,Gemma Ruiz-Olalla,Natalia Rodrigo Melero,Carlo Carolis,Daniel Parras,Pau Serra,Paula Martínez de Aguirre,Francisco Carmona-Torre,Gabriel Reina,Pere Santamaria,Alfredo Mayor,Alberto L García-Basteiro,Luis Izquierdo,Ruth Aguilar,Gemma Moncunill

Life sciences 225:55-63 PubMed30946839

2019

Activation of KRas-ERK1/2 signaling drives the initiation and progression of glioma by suppressing the acetylation of histone H4 at lysine 16.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzhen Wei,Fang Wang,Ben Sang,Zhen Xu,Dongxu Yang

Oncotarget 10:2252-2269 PubMed31040917

2019

PD-L1 checkpoint blockade delivered by retroviral replicating vector confers anti-tumor efficacy in murine tumor models.

Applications

Unspecified application

Species

Unspecified reactive species

Leah A Mitchell,Kader Yagiz,Andrew Hofacre,Sophie Viaud,Anthony W Munday,Fernando Lopez Espinoza,Daniel Mendoza,Maria E Rodriguez-Aguirre,Simon Bergqvist,Ali Haghighi,Marin V Miner,William P Accomando,Cynthia Burrascano,Dawn Gammon,Harry E Gruber,Douglas J Jolly,Amy H Lin

Nature communications 8:804 PubMed28986560

2017

The telomere binding protein Pot1 maintains haematopoietic stem cell activity with age.

Applications

Unspecified application

Species

Unspecified reactive species

Kentaro Hosokawa,Ben D MacArthur,Yoshiko Matsumoto Ikushima,Hirofumi Toyama,Yoshikazu Masuhiro,Shigemasa Hanazawa,Toshio Suda,Fumio Arai

The EMBO journal 34:1801-15 PubMed25979828

2015

TUT7 controls the fate of precursor microRNAs by using three different uridylation mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Boseon Kim,Minju Ha,Luuk Loeff,Hyeshik Chang,Dhirendra K Simanshu,Sisi Li,Mohamed Fareh,Dinshaw J Patel,Chirlmin Joo,V Narry Kim

mAbs 6:392-402 PubMed24492309

2014

HER2 monoclonal antibodies that do not interfere with receptor heterodimerization-mediated signaling induce effective internalization and represent valuable components for rational antibody-drug conjugate design.

Applications

Unspecified application

Species

Unspecified reactive species

Bart E C G de Goeij,Matthias Peipp,Simone de Haij,Edward N van den Brink,Christian Kellner,Thilo Riedl,Rob de Jong,Tom Vink,Kristin Strumane,Wim K Bleeker,Paul W H I Parren

PloS one 7:e45393 PubMed23028981

2012

Heterodimeric barnase-barstar vaccine molecules: influence of one versus two targeting units specific for antigen presenting cells.

Applications

Species

Unspecified reactive species

Heidi Cecilie Larsen Spång,Ranveig Braathen,Bjarne Bogen

Immunity 32:227-39 PubMed20171125

2010

In vivo requirement for Atg5 in antigen presentation by dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Heung Kyu Lee,Lisa M Mattei,Benjamin E Steinberg,Philipp Alberts,Yun Hee Lee,Alexander Chervonsky,Noboru Mizushima,Sergio Grinstein,Akiko Iwasaki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-6X His tag® antibody

ELISA - Biotin Anti-6X His tag® antibody (AB27025)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise