Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade

• WB

Lab

Western blot - Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB316173)

Western blot : Anti-His-tag antibody [EPR20547] (ab316173) staining at 1/1000 dilution, shown in black. In Western blot, ab316173 was shown to bind specifically to His-tag . First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 2 minutes 30 seconds exposure time. Secondary antibodies used were Peroxidase Streptavidin at 0.1 µg/mL.

All lanes:

Western blot - Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab316173) at 1/1000 dilution

Lane 1:

Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) <a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-spike-glycoprotein-s1-active-ab273068'>ab273068</a> cell lysate at 0.2 µg

Lane 2:

Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) <a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a> cell lysate at 0.2 µg

Secondary

All lanes:

Peroxidase Streptavidin at 0.1 µg/mL

Observed band size: 50 kDa,130 kDa

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