Rabbit Recombinant Monoclonal 6X His tag® antibody - conjugated to Biotin. Suitable for WB and reacts with Tag samples.
Images
![Western blot - Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB316173), expandable thumbnail](https://content.abcam.com/products/images/biotin-6x-his-tag-antibody-epr20547-chip-grade-ab316173--western-blot-img427772.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB316173)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Tag | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
2 products for Alternative Version
Target data
Alternative names
6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag
Recommended products
Rabbit Recombinant Monoclonal 6X His tag® antibody - conjugated to Biotin. Suitable for WB and reacts with Tag samples.
Alternative names
6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Biotin
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR20547
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- Activity summary
The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.
- Biological function summary
Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.
- Pathways
The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.
- Associated diseases and disorders
The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab316173), expandable thumbnail](https://content.abcam.com/products/images/biotin-6x-his-tag-antibody-epr20547-chip-grade-ab316173--western-blot-img427772.jpg "Western blot - Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab316173)")
Western blot - Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab316173)
Western blot: Anti-His-tag antibody [EPR20547] (ab316173) staining at 1/1000 dilution, shown in black. In Western blot, ab316173 was shown to bind specifically to His-tag . First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 2 minutes 30 seconds exposure time. Secondary antibodies used were Peroxidase Streptavidin at 0.1 µg/mL.
All lanes: Western blot - Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab316173) at 1/1000 dilution
Lane 1: Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) ab273068 cell lysate at 0.2 µg
Lane 2: Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) ab273530 cell lysate at 0.2 µg
SecondaryAll lanes: Peroxidase Streptavidin at 0.1 µg/mL
Performed under reducing conditions.
Observed band size: 50 kDa, 130 kDa
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